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  • Biology-Online • View topic - Where do protein complexes get their quarternary structure?
    will the holoprotein travel through the Golgi for further modifications or will the individual subunits or complexes of a few subunits travel through it and then finally be brought together where Thanks in advance if you have any link references please post Reply with quote venus666 venus666 Posts 8 Joined Wed Jun 24 2015 9 36 am Re Where do protein complexes get their quarternary structu Mon Oct 19 2015 2 31 am protein can have the hydrolysis reaction The hydrolysis reaction will happen through the stimulation of acid alkali or enzyme and finally it become several kinds of amino acids It is advised to find the breaking point of tissues when the protein is under the hydrolysis Resource http antigensandantibody blogspot hk 2015 10 the basic substance of human body html It might be helpful for you Reply with quote claudepa claudepa Posts 27 Joined Tue Nov 03 2015 12 43 pm Re Where do protein complexes get their quarternary structu Tue Nov 03 2015 4 49 pm I do not know the general answer However for transcription factors which are active through their quaternary structure this quaternary structure can only be obtained when activation by signal transmission pathways

    Original URL path: http://www.biology-online.org/biology-forum/about42023.html (2016-02-17)
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  • Biology-Online • View topic - CHOICE OF MAMMALIAN CELL HOST
    potentially pathogenic viruses 4 have genetic stability and 5 can be readily characterized with respect to karyology morphology isoenzymes and gene copy number The existence of a variety of host cell systems the availability of viral or cDNA based vectors and the possibility of either stable or transient expression requires that the prospective user define an expression strategy based on ultimate goals When the researcher s objectives require 1 mg of protein transient expression in COS 7 cells is the relevant route Transient expression in the COS cell and vaccinia systems has been recently reviewed Moss and Earl 1991 Aruffo 1997 and detailed protocols for construction of suitable vectors and protein expression by these systems can be found in those articles In transient expression a burst of production occurs in the host cell and is usually accompanied by death and rapid lysis of the cell This presents the purification scientist with the challenge of fishing out the protein of interest which may be present at 5 g ml from a soup of lysed cellular protein nucleic acids and viral particles The yield of product during purification may be low as a result of the low titer and starting purity however

    Original URL path: http://www.biology-online.org/biology-forum/about43063.html (2016-02-17)
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  • Biology-Online • View topic - Exocytosis vs Pinocytosis
    s the difference between they 2 PS My textbook named it as exopinocytosis I M SERIOUS Reply with quote Enzyme Enzyme Posts 336 Joined Mon Dec 26 2005 9 05 pm Sun Apr 16 2006 3 25 pm Exocytosis is the process by means of cells secrete different kinds of molecules content in a cytoplasmic vesicle from cytoplasm to extracellular intersticial space It occurs in all the eukaryotic cells and it implies the fusion of the vesicular membrane with the cytoplasmic membrane Exocytosis is very specialized in secreting cells and neurons Pynocytosis is the process by means of cells ingest liquid particles or dissolved particles from extracellular intersticial space to cytoplasm This process is very common in cells with nutritive function as cells of the intestinal mucose Alpha cells produce and secrete glucagon so the process is exocytosis At better in your textbook call them exopynocytosis because alpha cells can carry out the two processes independently They secrete glucagon by exocytosis but they can ingest dissolved substances by pynocytosis Do you understand what I mean Reply with quote kk kk Posts 64 Joined Fri Sep 23 2005 7 58 pm Mon Nov 02 2015 7 07 pm Question once a

    Original URL path: http://www.biology-online.org/biology-forum/about5970.html (2016-02-17)
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  • Biology-Online • View topic - PEG principle
    Location New York Website PEG principle Wed Oct 28 2015 9 44 am Hi recently I m planning to some experiments I found out that I do not know the PEG principle in cell fusion and the HAT principle in hybridoma cells culture clearly so can anyone explain it Thanks a lot Post a reply Jump to Select a forum General Biology General Discussion Cell Biology Molecular Biology Zoology Discussion

    Original URL path: http://www.biology-online.org/biology-forum/about43057.html (2016-02-17)
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  • Biology-Online • View topic - valve endothelial cells
    Joined Wed Oct 28 2015 9 28 am Location New York Website valve endothelial cells Wed Oct 28 2015 9 36 am Hi guys I m trying to extract a pig s valve endothelial cells but how to achieve the separation and extraction Thanks in advance Post a reply Jump to Select a forum General Biology General Discussion Cell Biology Molecular Biology Zoology Discussion Evolution Microbiology Bioinformatics Human Biology Botany

    Original URL path: http://www.biology-online.org/biology-forum/about43056.html (2016-02-17)
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  • Biology-Online • View topic - Cell Culture Contamination Air particle quantity/Size?
    building a simple DIY hood using a 20x20 box fan an air filter going to test multiple filters to find the minimum quality of filter and get the job done and an aluminum box I m a bit unsure of how good the BSL2 biosafety cabinets are at filtering contaminants Clearly my cultures don t get contaminated in a BSL2 biosafety cabinet but of course I can t imagine that the cabinet filter s out 100 of contaminents This guy has a air particle detector https www youtube com watch v kH5APw SLUU The best filter he has leaves 400 particles in the air I assume a BSL2Cabinet also leaves about the same So Does it take a single particle to contaminate a Culture If not what are the sort of ranges that would be safe for cell culturing Then what sort of MERV rating should I go for I m leaning towards merv 13 It gets much more expensive from there This site seems to claim that they can filter out all bacteria https filterbuy com pla product 20x20 Al138P8HAQ Cell Culture Contamination Air particle quantity Size ResearchGate Available from https www researchgate net post Cell ntity Size accessed

    Original URL path: http://www.biology-online.org/biology-forum/about43052.html (2016-02-17)
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  • Biology-Online • View topic - ATP and ADP+Pi
    Reply with quote kiekyon kiekyon Posts 410 Joined Thu Feb 16 2006 6 10 am Location Malaysia Mon Mar 13 2006 3 07 am one of the phosphate tail of ATP is transferred to other compound Pi so that leave two phosphate group ADP Reply with quote MrMistery MrMistery Posts 6832 Joined Thu Mar 03 2005 10 18 pm Location Romania small and unimportant country Mon Mar 13 2006 6 26 pm They are not the same thing ATP is one compound while ADP Pi are 2 separate compounds ADP Pi don t equal ATP just as 100 pieces of paper with writing on it don t equal a book In order to make ATP from ADP Pi you need to invest some energy just as you need to invest some energy to glue the pieces of paper together to make a book As a difference between my analogy and the biological situation turning your book into pages will not provide energy but turning ATP into ADP and Pi will Hope things are clear now Reply with quote harveydobson harveydobson Posts 1 Joined Mon Oct 19 2015 6 02 am Location UK Website Mon Oct 19 2015 6 08 am

    Original URL path: http://www.biology-online.org/biology-forum/about5320.html (2016-02-17)
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  • Biology-Online • View topic - cell biology
    Joined Thu Oct 15 2015 2 58 am cell biology Fri Oct 16 2015 12 43 am Actually it not so difficult to harvest ab and coating it onto plate Can you make them as well If available please send us the protocol for reference Post a reply Jump to Select a forum General Biology General Discussion Cell Biology Molecular Biology Zoology Discussion Evolution Microbiology Bioinformatics Human Biology Botany Discussion

    Original URL path: http://www.biology-online.org/biology-forum/about43019.html (2016-02-17)
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