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  • Biology-Online • View topic - ammonium and sulphates
    ammoniumsulphate is in the laboratory E coli multiplies in MgSO4 If I mix ammonium NH3 with E coli and MgSO4 Magnesium sulphate and maybe water do the E coli survive Reply with quote chemnorm chemnorm Posts 10 Joined Tue Feb 02 2016 6 18 am Website Wed Feb 03 2016 4 37 am Your experiments are designed to be novel and I have a complete understanding of it But I

    Original URL path: http://www.biology-online.org/biology-forum/about43036.html (2016-02-17)
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  • Biology-Online • View topic - My Multiplex PCR reaction works, but not my single rxn
    and I get a result I can t figure out a multiplex with four primers amplifies two products form DNA from a mixed infection as expected but one of the singleplex reactions i e using two primers designed for a single species doesn t work I can t figure out why that would be the case since I m running all the reactions in parallel preparing them at the same time using the same reagents using the same PCR machine etc I had one idea that I tested today but it didn t seem to work I thought maybe the one primer pair requires less MgCl2 than the other so that when it is with the other primer pair in a multiplex reaction as the other pair of primers amplifies its product maybe the MgCl2 gets sequestered in reactions with the first primer pair thus lowering the effective MgCl2 concentration for the pair that doesn t work by itself but I tried a MgCl2 gradient with the poorly performing primer pair today and it didn t seem to help Anybody have any ideas about what variables might be affected in a multiplex reaction that would cause a primer pair to amplify under those conditions but not when the reaction is done with only two primers Thanks Rod Reply with quote Javedz2001 Javedz2001 Posts 6 Joined Sun Oct 11 2015 4 32 pm Re My Multiplex PCR reaction works but not my single rxn Sun Oct 11 2015 4 48 pm Multiplex PCR is very dependent on MgCl2 and dNTP concentration Primer concentration also plays a big role For individual PCR try more primer and lower dNTP You should also add 10 DMSO in the reaction Lets us know if worked Good Luck Dr Javed Siddiqi Reply with quote Helics Helics

    Original URL path: http://www.biology-online.org/biology-forum/about42063.html (2016-02-17)
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  • Biology-Online • View topic - Glucose
    Aria94 Posts 1 Joined Wed Jan 20 2016 8 24 pm Glucose Wed Jan 20 2016 8 28 pm Hello everyone I was just confused about how Glucose can reduce Benedict s reagent when it is in it s ring form Many thanks in advance Post a reply Jump to Select a forum General Biology General Discussion Cell Biology Molecular Biology Zoology Discussion Evolution Microbiology Bioinformatics Human Biology Botany Discussion

    Original URL path: http://www.biology-online.org/biology-forum/about43327.html (2016-02-17)
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  • Biology-Online • View topic - Cell volume and reciprocal cell volume calculations? whaaat?
    quote drivenmaker12 drivenmaker12 Posts 1 Joined Thu Jan 07 2016 11 18 am Cell volume and reciprocal cell volume calculations whaaat Thu Jan 07 2016 11 27 am I had a lecture today that included cell volume and reciprocal cell volume calculations and I did not get it at all We went through two problems in class and I was wandering if anyone can shed some light on them at all like what they are and how you would actually work them out I have loads of these calculations to do during my course and I really need to get to grips with them Problem 1 A protein crystallises in a monoclinic unit cell with cell parameters a 100 02Å b 90 57Å c 68 33Å 90 0o 104 48o 90 0o What is the cell volume and what is the reciprocal cell volume Problem 2 A protein crystallises in an orthorhombic unit cell with cell parameters a 45 69Å b 150 22Å c 75 87Å 90 0o What is the cell volume and what is the reciprocal cell volume Post a reply Jump to Select a forum General Biology General Discussion Cell Biology Molecular Biology Zoology Discussion Evolution Microbiology

    Original URL path: http://www.biology-online.org/biology-forum/about43300.html (2016-02-17)
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  • Biology-Online • View topic - HFQ Protein from bacteria
    an appropriate concentration of imidazole I have also extracted the total RNA from Proteus mirabilis using Trizol procedure I am ready for the Protein RNA binding assay but i need help in a standardized procedure to execute the Protein RNA binding assay Thank you Reply with quote claudepa claudepa Posts 27 Joined Tue Nov 03 2015 12 43 pm Thu Dec 31 2015 4 21 pm Hi I am sure you will have a precise answer from a researcher using this technic I am only curious about sRNA I know many RNAs but not this one I suppose that the principle of the technic after binding is the digestion of unprotected RNA by RNase Then digestion of the protein by a protease and finally analysis of the protected RNA Have a good work Reply with quote kishsel kishsel Posts 2 Joined Wed Dec 30 2015 6 28 am Re HFQ Protein from bacteria Sat Jan 02 2016 12 36 pm sRNA are bacterial small non coding RNAs My objective is in the identification and characterization of Proteus mirabilis HFQ protein binding small non coding RNAs Reply with quote claudepa claudepa Posts 27 Joined Tue Nov 03 2015 12 43 pm

    Original URL path: http://www.biology-online.org/biology-forum/about43279.html (2016-02-17)
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  • Biology-Online • View topic - Brunner Syndrome: could truncated MAO protein cause problem?
    itself could cause problems In other words is it the absence of monoamine oxidase that causes the problems or the presence of truncated monoamine oxidase From what I can tell it is the excess of neurotransmitters that is more likely to cause the problems My main reason for thinking so other than that s what the relevant researchers are saying is that there appear to have been up to three different varieties of mutation in the MAO A gene all of which cause the same basic phenotype It s just for a sense of completeness in writing about the topic that I want to say as scientists we need to consider all possibilities and one possibility is that the truncated protein causes the problem Then more science evidence suggests no but it s good that we looked down that route just to be sure My concern is that there might be something ignorant about saying this For example does the body have a clean up system to get rid of truncated proteins Again doesn t seem likely but it s an example of something I might not have thought about which would therefore make me look like an idiot if I suggest the potential role of the truncated protein Some relevant reading A good overview of Brunner syndrome and other material on the potential link between MAOA and violence can be read in chapter 12 of the below linked book Skip to p 179 most all of the chapter is available free in the google books preview https books google co uk books id Bmy ne f false Novel monoamine oxidase A knock out mice with human like spontaneous mutation https www ncbi nlm nih gov pmc articles PMC3435113 New insights into Brunner syndrome and potential for targeted therapy http

    Original URL path: http://www.biology-online.org/biology-forum/about43198.html (2016-02-17)
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  • Biology-Online • View topic - Why is TSH reported in mIU/l
    Joined Fri Feb 15 2008 5 38 pm Location Philomath Oregon USA Website Re Why is TSH reported in mIU l Wed Nov 18 2015 4 21 pm If you have an easily denaturable protein or peptide which I ll call stuff different preparations will have different ratios of active good stuff to non functional crappy stuff If you define your experiments in terms of mg of the stuff you will end up with no consistency of the activity of the stuff between batches because different batches will have different good to crappy ratios However if you test the stuff to see how well it works and measure out amounts based on activity the you can reproduce your experiments even when you run out of stuff and have to buy another bottle with its new and different ratio of good stuff to crappy stuff I don t know how the function test for TSH is done You could convert the activity to mass if you know the ration of good stuff to crappy stuff but likely you don t have that number Reply with quote gs99 gs99 Posts 38 Joined Thu Dec 31 2009 2 48 pm Re Why is TSH reported in mIU l Wed Nov 25 2015 5 54 pm I don t have lab experience I have lab reports of my blood and am curious why thyroxine hormone is reported as mass volume but Thyroid Stimulating Hormone is stated in mIU volume If this is an international unit why doesn t anybody know what it is Reply with quote jonmoulton jonmoulton Posts 442 Joined Fri Feb 15 2008 5 38 pm Location Philomath Oregon USA Website Re Why is TSH reported in mIU l Thu Dec 10 2015 3 36 pm https en wikipedia org wiki International

    Original URL path: http://www.biology-online.org/biology-forum/about43092.html (2016-02-17)
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  • Biology-Online • View topic - Define microbicidal
    are a defensing microbicidal peptides If biocides render microbes such as viruses harmless how do microbicidals reduce ineffective of microbes Microbicidal is defined as any biocidal compound or substance whose purpose is to reduce the ineffective of microbes such as viruses or bacteria One example is wood tar Biocidal is defined as chemical substance or microorganism render harmless effect on any harmful organism by chemical or biological means Post a

    Original URL path: http://www.biology-online.org/biology-forum/about43179.html (2016-02-17)
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