archive-org.com » ORG » B » BIOLOGY-ONLINE.ORG

Total: 791

Choose link from "Titles, links and description words view":

Or switch to "Titles and links view".
  • Biology-Online • View topic - Recommendations for Bacteria Detection?
    am in obtaining some idea of bacteria density or parts per gram I m not sure if it matters but the water may be contaminated with fertilizer soaps etc Is there some kind of quick test that can help me out with that Or is the best way to get some sort of field microscope and put a sample on a glass slide Thank you very much for your expertise Lodestone Reply with quote Cat Cat Posts 635 Joined Thu Feb 14 2008 7 40 pm Sat Sep 20 2014 4 29 pm I doubt very much that you can do it in the field I think you need to culture and do colony counts the old fashioned way Reply with quote josem josem Posts 36 Joined Sun Jun 08 2014 7 58 pm Tue Oct 07 2014 7 58 pm I think you can use a hemocytometer to count the total bacteria present in the water But it is difficult to count the motile bacteria in hemocytometer I suggest to use a disinfectant to kill the bacteria before counting I think you can also use a stain for seeing the bacteria in the microscope Reply with quote JackBean JackBean Posts 5694 Joined Mon Sep 14 2009 7 12 pm Wed Oct 08 2014 9 34 am Well the standard way is to filter the water and keep the filter on some growth medium to see how many colonies will form However not every growth medium will suit every bacteria and some bacteria are unculturable in general More precise way is to do 16S RNA sequencing but I m not sure if that tells also the amount of bacteria or only the species Reply with quote SpellForce SpellForce Posts 4 Joined Thu Oct 02 2014 7 59 pm Wed Oct

    Original URL path: http://www.biology-online.org/biology-forum/about40135.html (2016-02-17)
    Open archived version from archive


  • Biology-Online • View topic - immobilized metal affinity chromatog. for bacterial lysate
    affinity chromatog for bacterial lysate Mon Apr 06 2015 7 51 am Hi all i have planned to perform IMAC to study intracellular phosphoproteins in bacterial lysate and have decided to purchase the column could i get any advice on the purchase of IMAC column from this forum i expect that there are some specific criteria of the column for bacterial lysate your kind help is highly appreciated thank you

    Original URL path: http://www.biology-online.org/biology-forum/about41765.html (2016-02-17)
    Open archived version from archive

  • Biology-Online • View topic - GCMS analysis of bacterial cells
    treatment with antibacterial compound i really appreciate if someone can help me on how to prepare the sample prior to GCMS analysis Please correct my procedure below if i am wrong ya 10q a preparation of bacterial culture b centrifugation to get bacterial cells pellet c standardization of bacterial density in 1 000 000 CFU mL between non treated control and treated sample d derivatization using relevant solvent in my

    Original URL path: http://www.biology-online.org/biology-forum/about41763.html (2016-02-17)
    Open archived version from archive

  • Biology-Online • View topic - Does this could be a Spreading way of Chagas disease?
    defecates depositing the infective feces on the skin of the host so that wen the host rubs the skin as the feces causes that area to itch T cruzi now gets the chance to enter the host body thru open wounds mouth or eyes However gettng the disease thru currencies seems a bit unlikely UNless it got contaminated with the bugs feces then accidentally ingesting it Rather eating an unsanitary uncooked food directly contaminated with infective bug feces is more probable mode of acquiring spreading that disease if by a chance that traveler came in contact with infective bug feces and unknowingly ingested it chances are that traveler could be harboring the parasite other modes of transmission are by receiving infected blood transfusion or congenitally Reply with quote Denise Denise Posts 2 Joined Wed Mar 21 2007 3 48 pm Chagas Disease questions Wed Mar 21 2007 4 06 pm I recently returned from a trip to Placencia Belize One the third day I woke up with a swollen red area around my right eye Later in the day I noticed a C shaped burn on my leg and an ovel shaped burn on my waste on my back Later the same day I noticed my right knee appeared to be sunburned The next day liquid blisters appeared on them and they begin to itch A week later they are no longer red but look like large bruises and still itch I have noticed a small rash on my wrist and one on my right elbow I visited my doctor and he couldn t diagnose it He referred me to a dermatologist and he didn t know I am going in for bloodwork today Does this sound like Chagas Disease Reply with quote Darby Darby Posts 1274 Joined Thu Mar 02 2006 5 29 pm Location New York USA Wed Mar 21 2007 6 01 pm Denise your symptoms don t match Chagas Disease at all Since trypanosomes do not form cystlike structures their ability to survive any kind of drying environment would be very limited transfer by money would be very unlikely Usually they depend upon contamination of the bite wounds with feces from the biter Reply with quote Denise Denise Posts 2 Joined Wed Mar 21 2007 3 48 pm Wed Mar 21 2007 6 32 pm Thanks to your quick response Do you have any suggestions on what it could be I was prescribed a topical steroid but haven t seen any changes I never ran fever vomited or had a headache I ve looked up Belize skin rashes and found some similiaties but no real solution or answer Reply with quote Darby Darby Posts 1274 Joined Thu Mar 02 2006 5 29 pm Location New York USA Thu Mar 22 2007 11 19 pm No idea with only those symptoms there isn t much that matches Reply with quote CindySmith CindySmith Posts 1 Joined Mon Mar 30 2015 9 41 am Location Hoover AL Website Re

    Original URL path: http://www.biology-online.org/biology-forum/about8305.html (2016-02-17)
    Open archived version from archive

  • Biology-Online • View topic - Topical Antibiotic Use (Bactroban)
    a few questions The impetigo literally ate away at the skin on my chin to the point where any hair I had on my chin had fallen off with the impetigo crusts Is it really safe to use it on such raw new skin Why is is not recommended to use the ointment around the mouth area I m worried to use it on my chin because of this The impetigo also affected my bottom lip I was using antibacterial honey on my rash Would quitting the honey and introducing another antibiotic Bactroban cause any issues Too much antibacterial activity for my chin The ointment says to use a small amount and that using too much could cause fungal environment cause damage to my kidneys etc However the rash is on my entire chin so I d probably have to use a lot of the ointment anyway Anything I can do to prevent the side effects since I have to use so much of it I was prescribed to use it 3 times a day for 7 days I think Do I have to treat this like I would an oral antibiotic like applying it at the same time daily

    Original URL path: http://www.biology-online.org/biology-forum/about41732.html (2016-02-17)
    Open archived version from archive

  • Biology-Online • View topic - Xenobiotic Agents for Biofilm Prevention and Treatment
    and Treatment Tue Mar 03 2015 5 38 pm I came cross an online post on the Infectious Disease and Immunology Research Institute at Loyola University Chicago There are some interesting research results regarding the biofilms prevention and treatment from Karen L Visick PhD and James J Jaber MD PhD Here is the whole post http stritch luc edu depts indii research projects novel xenobiotic agents for biofilm prevention and treatment in chronic rhinosinusitis Biofilms are a serious problem in the clinic due to three key factors 1 the extracellular matrix limits diffusion of molecules such as antibiotics preventing them from readily accessing the target bacteria 2 some bacteria within the biofilm are dormant which diminishes the effectiveness of traditional antibiotics that kill actively metabolizing cells and 3 the ability of the bacteria to readily tightly adhere to surfaces and to each other limits the effectiveness of immune system However current treatment protocols fail to take into account all 3 well known factors listed above that are involved in the inherent resistance to antimicrobials of bacteria within biofilms Therefore our goal is to develop a novel therapeutic agent that incorporates plans for combatting each of these resistance mechanisms in the

    Original URL path: http://www.biology-online.org/biology-forum/about41626.html (2016-02-17)
    Open archived version from archive

  • Biology-Online • View topic - antibiotics vs antibacterial drugs..
    includes antibiotics and synthetic materials Reply with quote Xaolou Xaolou Posts 5 Joined Mon Mar 12 2012 8 04 am Re antibiotics vs antibacterial drugs Wed Mar 28 2012 8 17 am That is true Yet the term were used synonymously in the earlier days so that s why you might mix them up A much more important distinction however is the distinction between antibodies and antibiotics Whereas antibodies are produced by the body to destroy invading bacteria antibiotics are chemically produced structures to do a similar thing But it should be noted that antibodies are nowadays also chemically produced and provided by an antibodies supplier for the purpose of research Humankind more and more tries to imitate the body s mechanisms to fight viral and bacterial infections Reply with quote JorgeLobo JorgeLobo Posts 424 Joined Mon Nov 17 2008 12 12 am Wed Mar 28 2012 11 49 am I suppose we could find some folks including naive students as here considering these to be synonymous but certainy not in earier days any more than now and not among folks of with technical knowledge of the subject The question did not address antibodies Of course there s a much greater distinction betwen antbodies and antibiotics Humankind develops drugs constantly and no more by imitation of the body than before I m not aware of significant chemical synthesis of antibodies though certainly of associated therapeutic ligands Can you say more Reply with quote alQpr alQpr Posts 1 Joined Wed Feb 04 2015 12 09 pm Re antibiotics vs antibacterial drugs Wed Feb 04 2015 12 54 pm To the person who said Why is it so hard ok smartass then why are the answers at ResearchGate all over the map From the top stackexchange answer it appears that antibiotic was

    Original URL path: http://www.biology-online.org/biology-forum/about24257.html (2016-02-17)
    Open archived version from archive

  • Biology-Online • View topic - Science vs. Science Fiction, non-scientist needs help
    bacterial colony growing in it This colony has evolved to form a kind of collective intelligence They consume iron For the purposes of story telling I made them bioluminescent I further made their collective intelligence an adaptation of bioluminescent communication which I know is sometimes used for quorum sensing but perhaps a different adaptation would make collective intelligence more believable I don t know I believe you are not saying anything out of this world lol However I have some reservations regarding the collective intelligence Here is some info on some of the points Bacteria often use iron though often this comes from a host http onlinelibrary wiley com doi 10 1016 0307 4412 83 90043 2 pdf http www dbi ca books PDFs Water Paper PDF Bioluminescent bacteria often use molecules such as Luciferase http en wikipedia org wiki Luciferase https microbewiki kenyon edu index ph n Settings and http cibt bio cornell edu workshops a Biolum pdf Cell to cell interaction http en wikipedia org wiki Cell E2 80 n bacteria However I see two major problems as you can see from the links above cells interact with eachother This often comes through a physical or chemical queue However this should happen within a very small distance so that the cells could detect such queues But this is no major concern I m guessing Imagine that they are all in a very dark cave if there is no light any bioluminescent queue would be easily seen Interestingly the first bacteria where Luciferase was observed did not produce this luminescent response when growing in small numbers but when big colonies occurred this luminescent response was observed http en wikipedia org wiki Autoinducer Discovery How intelligent would they be This is the major concern that I have The sole objective of most unicellular organisms such as bacteria is to grow as fast as possible This allows organisms to outcompete the slower growing ones eventually It is difficult to say that such a small and simple organism such as a bacteria is intelligent But if the kind of intelligence that you are looking for would be simple I believe it would be plausible I m going to let my mind fly now Imagine that throughout evolution these colonial bacteria could develop a system in which they would produce a response i e luminescence under stress This would mean that if the environment changed for worse these bacteria could start sending that response to the neighbouring cells These would detect these cells and enter a state where their defenses were stronger though their growth would be slower I believe all of this makes sense though a luminescent response implies a high energetic cost that problem the cells cannot afford greatly if their environment changes for worse Also I believe though I haven t searched about this that cell to cell interaction is extremely simple in lower organisms Nevertheless lower organisms still develop in colonies so it is entirely possible that they interact

    Original URL path: http://www.biology-online.org/biology-forum/about40406.html (2016-02-17)
    Open archived version from archive



  •