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  • Biology-Online • View topic - dilutionMagic - serial dilution calculator
    on a 96 well plate Currently it works on Windows It can do custom dilution steps and non equal sample volumes The project is and will remain open source The beta version is operational but I need user feedback to make it better Please download the program and try it out at www dilutionmagic org You can tell me what you think and what you would like to be improved

    Original URL path: http://www.biology-online.org/biology-forum/about43357.html (2016-02-17)
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  • Biology-Online • View topic - Upcoming Workshop: A Beginner's Guide to NGS Data Analysis
    purpose of this workshop is to get a deeper understanding in Next Generation Sequencing NGS with a special focus on bioinformatics issues Additionally all workshop participants should be enabled to perform important tasks of NGS data analysis tasks themselves The first workshop module is an introduction to data analysis using Linux assuring that all participants are able to follow the practical parts The second module dicusses advantages and disadvantages of current sequencing technologies and their implications on data analysis The most important NGS file formats fastq sam bam bigWig etc are introduced and one proceeds with first hands on analyses QC mapping visualization You will learn how to read and interprete QC plots clip adapter sequences and or trim bad quality read ends get bioinformatics backgrounds about the read mapping and understand its problems dynamic programming alignment visualization NGS mapping heuristics etc perform your own mapping statistics and visualize your data in different ways IGV UCSC etc The last two modules adress two specific applications of NGS RNA seq of model organisms and RNA seq of non model organisms Find more information on the workshop website http www ecseq com workshops workshop 2015 01 html Reply with quote biologyhopkins biologyhopkins Posts 4 Joined Tue Apr 14 2015 2 57 pm Tue Apr 21 2015 3 37 pm I m sorry I missed this Have a healthy interest in data visualization and I could have easily made it to Leipzig Shame Reply with quote biologyhopkins biologyhopkins Posts 4 Joined Tue Apr 14 2015 2 57 pm Wed Apr 29 2015 3 23 pm In fact I ve recently come across this data vizualization software which I know my school s lab has been using for analysis Take a look They make the dashboard really simple so you can concentrate on the

    Original URL path: http://www.biology-online.org/biology-forum/about41428.html (2016-02-17)
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  • Biology-Online • View topic - bioinformatics study partner
    research Post a reply Reply with quote karimse07 karimse07 Posts 1 Joined Sun Feb 15 2015 5 04 am bioinformatics study partner Sun Feb 15 2015 5 08 am need a bioinformatics online study partner skype karimse07 Post a reply Jump to Select a forum General Biology General Discussion Cell Biology Molecular Biology Zoology Discussion Evolution Microbiology Bioinformatics Human Biology Botany Discussion Genetics Physiology Ecology Biology Documents Biology Movies PDF

    Original URL path: http://www.biology-online.org/biology-forum/about41506.html (2016-02-17)
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  • Biology-Online • View topic - Questions about DNA dialysis
    exchange ions in my DNA For this I bought Slide A Lyzer mini pots for the dialysis First I am looking for more information about the theoretical background of dialysis Can you give me some literature hints please Secondly I know my target DNA concentration but I know dialysis can lead to dilution and sample loss How much higher shall I choose the starting concentration Also how can I calculate

    Original URL path: http://www.biology-online.org/biology-forum/about41477.html (2016-02-17)
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  • Biology-Online • View topic - Taqman probe design using FISH probe (need help)
    5 Joined Wed Feb 04 2015 7 55 am Location 45 16 Ramsey Road Shirley NY 11967 USA Website Wed Feb 04 2015 8 11 am Hi Although they are both called probes FISH and Taqman probes have different characteristics Probes for FISH need to be at least 1 kb long to hybridize and highlight the target chromosomal DNA metaphase chromosomes or interphase nuclei Hydrolysis probes such as Taqman are very short and used in combination with other two oligonucleotides the size of the amplicon is very small usually 200 bp here the hydrolysis probe has an annealing temperature such that it will remain hybridized at PCR extension temperature but not so high as a fish probe and will be hydrolysed by Taq DNA polymerase at every replication thus releasing the fluorescence quenched in intact probe that will be measured to quantify the starting level of template Therefore you can design primers and probes so that they will fall within the sequence recognized by the FISH probe you can easily find instructions for designing good quality primers for qPCR Good luck Post a reply Jump to Select a forum General Biology General Discussion Cell Biology Molecular Biology Zoology Discussion Evolution

    Original URL path: http://www.biology-online.org/biology-forum/about41474.html (2016-02-17)
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  • Biology-Online • View topic - Mastermix in PCR
    on PCR run going to be Would it do anything else than maybe promoting the reaction further because the concentration is higher I cannot think of something since I am not that familiar with PCR work Please help me out by sharing you knowledge I really appreciate your time guys Reply with quote JackBean JackBean Posts 5694 Joined Mon Sep 14 2009 7 12 pm Tue Oct 02 2012 8 01 am If it was 1 25 ul in 50 ul total I wouldn t be much concerned However I would probably not compare these results to others with standard set up Reply with quote daddyjames daddyjames Posts 4 Joined Sun Oct 14 2012 1 57 pm Sun Oct 14 2012 3 43 pm I agree with what JackBean said however it does not answer your question MgCl2 is a required cofactor for the Taq Polymerase to work The activity of the Taq polymerase is sensitive to the concentration of MgCl2 The higher the concentration of the MgCl2 the more active the enzyme is As the concentration of MgCl2 increases the incorporation of dNTPs increases However it also increases the the error rate the rate at which an incorrect dNTP is incorporated into the resulting PCR product as compared with the original sequence MgCl2 also can stabilize dsDNA structures Manipulation of MgCl2 concentration increasing it is often used to increase amplification of hard to amplify DNA samples However this increases the amplification of non specific PCR products PCR products that are formed from the stabilization of the PCR primers binding to slightly different DNA sequences which would not occur under lesser concentrations of MgCl2 Reply with quote stonjohn4 stonjohn4 Posts 1 Joined Sat Dec 08 2012 4 27 am Sat Dec 08 2012 4 28 am Hi All I am

    Original URL path: http://www.biology-online.org/biology-forum/about26341.html (2016-02-17)
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  • Biology-Online • View topic - Microarray experimental design
    2014 12 21 pm Microarray experimental design Wed Oct 15 2014 12 32 pm Hi I m new in Bioinformatics my background is in Computer Science and I m taking a course to introduce myself in this area One of the exercises of the course is about designing an experiment in microarray technology The experiment should find if three compounds that had been used to treat a disease have potential adverse effects To accomplish the experiment we have access to samples from a range of tissues taken from three mice treated by the three different compounds during 7 days The samples from first and second compounds were taken in seventh day and the samples of the third compound were taken in the tenth day I know that I need to calculate the number of samples to use in the array and number of the biological replicates but I don t even know which questions the experiment should answer I don t want the possible solutions to the problem I just need that someone light me a little bit Thanks for your time Post a reply Jump to Select a forum General Biology General Discussion Cell Biology Molecular Biology Zoology Discussion

    Original URL path: http://www.biology-online.org/biology-forum/about41085.html (2016-02-17)
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  • Biology-Online • View topic - Database of Multicellular and Unicellular organisms
    a list of organisms taken from NCBI for cellularity unicellular or multicellular Does any of you ever found a database with association between an organisms name or taxa and whether it is unicellular or multicellular Desirably I would like to get information both on species level and on higher taxonomic level phyla classes etc but any information on this is welcomed In case it matters I m using Perl to

    Original URL path: http://www.biology-online.org/biology-forum/about39925.html (2016-02-17)
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