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  • Biology-Online • View topic - Knockdown vs Knockout
    heritable change that will yield a model organism line you can use for years go with a knockout If you are targeting an embryonic lethal gene you will probably need to use a knockdown You can either use a reduced dose of the knockdown agent leading to partial loss of target gene expression or use a knockdown agent that can be dosed or activated after the period of embryonic lethality For a dose response study of partial to complete loss of the target gene expression you would need a knockdown Knockouts can cause changes in expression of non target genes as an organism adapts to the loss of the targeted gene These might include physiological and epigenetic changes as well as over generations selection for compensatory mutation Knockdowns cause fairly rapid changes on a shorter time scale than knockouts Reply with quote jonmoulton jonmoulton Posts 442 Joined Fri Feb 15 2008 5 38 pm Location Philomath Oregon USA Website Re Knockdown vs Knockout Tue Jul 21 2015 6 18 pm Here is some very recent information about knockdowns versus knockouts Genetic compensation as adjustments in gene expression to ease the impact of a deleterious mutation are observed by this group

    Original URL path: http://www.biology-online.org/biology-forum/about41992.html (2016-02-17)
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  • Biology-Online • View topic - Raw Materials: Heritable variation among individuals
    NY 11967 USA Website Wed May 13 2015 2 33 am A gene is a series of codons along part of a DNA molecule that together will code for a protein One gene actually can code for more than one protein It depends on how you read the code in the DNA how you mature the mRNA s splicing and how you translate and modify the resulting protein s It

    Original URL path: http://www.biology-online.org/biology-forum/about41669.html (2016-02-17)
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  • Biology-Online • View topic - Souther blot and restriction mapping
    probe was a 2 4 kb XhoI fragment Using the southern data determine the restriction map for genomic DNA Data Enzyme Approximate Band Sizes E 6 0 1 8 X 2 4 B 10 3 6 XE 1 7 0 7 XB 2 3 0 1 BE 2 0 1 6 0 2 Any help with this would be greatly appreciated Thank you Reply with quote JackBean JackBean Posts 5694 Joined Mon Sep 14 2009 7 12 pm Wed Oct 08 2014 9 57 am What I did was to draw several lines each with marking the placeswhere the enzymes must cut and space in between X must be Xho obviously as it gives exactly one band of the same size so X E gives 0 7 1 7 X B gives 2 3 0 1 B E gives 2 0 1 6 0 2 if you compare XE and XB you can get either 1 6 fragment in between with my positioning or 0 6 fragment with the opposite position of fragments in XE i e 1 7 0 7 Now you just have to put it together X 0 7 E 1 6 B 0 1 X you know the X must be on the borders and thus E and B cut in between Now it s just matter of trying where B and E may cut to fit the pattern of single digest E has 1 8 fragment which coincides with 1 6 0 1 0 1 so E cuts 0 1 to the right from the right X site and 5 3 to the left from left X site B has 3 6 fragment which is made together from 1 6 BE 0 7 XE and 1 3 invisible in BX digest but combination with previous

    Original URL path: http://www.biology-online.org/biology-forum/about40976.html (2016-02-17)
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  • Biology-Online • View topic - genotype ratio and phenotype ratio in X linked allege
    18 2015 8 42 pm genotype ratio and phenotype ratio in X linked allege Wed Mar 18 2015 8 57 pm Ok I how would I write out the genotype and phenotype for a X link recessive gene when after the punnett square the two females are not effect and the two males are effected Post a reply Jump to Select a forum General Biology General Discussion Cell Biology Molecular

    Original URL path: http://www.biology-online.org/biology-forum/about41683.html (2016-02-17)
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  • Biology-Online • View topic - genotypic ratio and phenotypic ratio
    ratio and phenotypic ratio Wed Mar 18 2015 8 53 pm Ok what would the genotype ratio and phenotype ratio be in the problem below RT Rt rT rt Rt RRTt RRtt RrTt Rrtt Rt RRTt RRtt RrTt Rrtt Rt RRTt RRtt RrTt Rrtt Rt RRTt RRtt RrTt Rrtt R round r wrinkled T tall t short Post a reply Jump to Select a forum General Biology General Discussion Cell

    Original URL path: http://www.biology-online.org/biology-forum/about41682.html (2016-02-17)
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  • Biology-Online • View topic - CRISPR/Cas technology
    am Does anyone have experience with generating knockout mice with CRISPR Cas technology I have ordered knockout ES cells for my protein and we reached the chimeras stage male and female 70 approximately However I am not getting heterogeneous mice from the chimeras I wanted to utilize CRISPR technology Is it doable in the lab or would we have to outsource to a company to obtain the knockout ES Post

    Original URL path: http://www.biology-online.org/biology-forum/about41681.html (2016-02-17)
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  • Biology-Online • View topic - gene knockout mice
    knockout mice Wed Mar 18 2015 6 22 am Is it possible to get the striatum astrocyte specific Cre recombinase mediated knockout of XX gene knockout mice Any one understand how to make the knockout locate in striatum or any other good ideas to knockout one gene in one special cell from one specia area Post a reply Jump to Select a forum General Biology General Discussion Cell Biology Molecular

    Original URL path: http://www.biology-online.org/biology-forum/about41680.html (2016-02-17)
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  • Biology-Online • View topic - How to stimulate hepatocyte proliferation in vivo
    w19910411 w19910411 Posts 6 Joined Wed Mar 11 2015 8 41 am How to stimulate hepatocyte proliferation in vivo Wed Mar 18 2015 6 17 am I am looking for an alternative to partial hepatectomy and irradiation to induce hepatocyte proliferation in wild type mice Post a reply Jump to Select a forum General Biology General Discussion Cell Biology Molecular Biology Zoology Discussion Evolution Microbiology Bioinformatics Human Biology Botany Discussion

    Original URL path: http://www.biology-online.org/biology-forum/about41679.html (2016-02-17)
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