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  • Botany & Mycology 2009 - Abstract Search
    or not a SELECT 12 47 05 Abstract Detail Symbioses Plant Animal and Microbe Interactions Joneson Suzanne 1 Lutzoni Francois 1 A Fungal and Algal Perspective on Genes Upregulated in Early Lichen Development Our understanding of symbiotic interactions between members of the kingdoms Chloroplastida and Fungi comes primarily from studies involving vascular plants and their fungal symbionts To determine how broadly genetic mechanisms of symbiosis between members of the Chloroplastida and Fungi are conserved a wider sampling within both kingdoms needs study Although the fungal perspective from interactions with vascular plants has been inspected from a diverse number of fungi we do not know what to expect from fungi who are symbiotic with green algae Based on the signaling pathways that land plants use in response to fungal pathogens and mutualists and based on the limited information we have regarding the presence or absence of these same signaling pathways in green algae it is possible that green algae may respond to fungal symbionts in novel ways We present here fungal and algal genes involved in the development of lichen symbiosis between the single celled green alga Asterochloris sp and the filamentous fungus Cladonia grayi discovered using SSH and verified with

    Original URL path: http://2009.botanyconference.org/engine/search/index.php?func=detail&aid=267 (2016-02-01)
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  • Botany & Mycology 2009 - Abstract Search
    database 12 47 08 SQL Statement is null or not a SELECT 12 47 08 Abstract Detail Genetics Section Weiblen George D 1 Craft KJ 2 Wenger Jonathan P 3 Mehmedic Z 4 ElSohly M 4 A genetic linkage map for Cannabis based on microsatellites and AFLP markers We screened an inbred population of Cannabis sativa using microsatellite and AFLP markers for the purpose of mapping quantitative trait loci QTL in this economically important species Dioecious hemp and marijuana cultivars were subject to five generations of full sib inbreeding and crossed to produce an F1 mapping population A single F1 was selfed using silver nitrate to induce fertile male flowers in a genetically female plant F2 plants genotyped for microsatellite and AFLP markers were used to construct a linkage map with JoinMap software Linkage groups segregating with drug content and other economically important traits were identified Screening of the F2 population using single nucleotide polymorphisms SNPs from an expressed sequence tag EST library of hemp and marijuana line is proposed to better resolve major QTL Log in to add this item to your schedule 1 University of Minnesota Department of Plant Biology 250 Biological Sciences 1445 Gortner Avenue St Paul

    Original URL path: http://2009.botanyconference.org/engine/search/index.php?func=detail&aid=918 (2016-02-01)
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  • Botany & Mycology 2009 - Abstract Search
    1 Wojciechowski Martin F 3 A Genus Level Phylogeny of the Inverted Repeat Lacking Clade of Legumes The Inverted Repeat lacking clade IRLC containing 52 genera and over 4 400 species is the largest and most widespread clade of papilionoid legumes Leguminosae The loss of one copy of the inverted repeat in their plastid genomes is a synapomorphy for the IRLC in addition to a number of morphological and anatomical features The IRLC includes a number of important agricultural crops such as the garden pea Pisum alfalfa Medicago fava bean Vicia clover Trifolium genetic genomic model species e g Medicago truncatula and model systems for the study of nodulation nitrogen fixation by rhizobia Phylogenetic relationships within this group remain poorly understood due to its size limited sampling and widespread temperate distribution Certain groups within this clade have been previously recognized but their relationships are not well resolved or supported Previous studies indicate the Asian genera Callerya Wisteria and Glycyrrhiza form the earliest branches in the clade These same studies suggest there are three major subclades one composed of Pisum Medicago Trifolium Vicioid clade and relatives one Astragalean clade which contains the genus Astragalus 2 500 spp and its closest relatives and a third Hedysaroid clade which contains the genus Hedysarum and related genera Sequences of the nuclear rDNA ITS region and the plastid matK gene were obtained from representatives of IRLC genera not sampled previously and combined with a large number of sequences already in GenBank approx 1000 plus appropriate outgroups in Loteae and Robinieae Sequences of matK were analyzed using standard methods i e parsimony for the purpose of resolving relationships across the entire IRLC and its larger subclades Sequences from nuclear rDNA ITS were analyzed with a focus on resolving relationships specifically within the larger subclades Results from

    Original URL path: http://2009.botanyconference.org/engine/search/index.php?func=detail&aid=788 (2016-02-01)
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  • Botany & Mycology 2009 - Abstract Search
    is null or not a SELECT 12 47 23 Abstract Detail MSA Ecology Pathology Klich Maren 1 A greenhouse method for raising cotton susceptible to Aspergillus flavus infection Aflatoxin a highly carcinogenic secondary metabolite produced by Aspergillus flavus is a chronic problem in seeds of cotton raised in the hot dry low desert areas of the southwestern USA Genetically modified A flavus resistant cotton cultivars are being developed for control of aflatoxin A greenhouse system to test the efficacy of these cultivars for control of A flavus infection of uninjured bolls is needed To achieve this a series of experiments have been conducted on non transformed cotton plants to determine the conditions most conducive to A flavus infection of uninjured plants Conditions were developed to mimic western field grown cotton and the plants were subjected to environmental factors known to increase A flavus infection These included using soil filled white PVC pipes 136 cm tall x 15 cm diam as planters which allow tap root development a misting system to imitate dew water stress nighttime temperatures above 25 C and presentation of inoculum to the floral subfloral nectaries with an artist s brush to mimic wind blown spores This method

    Original URL path: http://2009.botanyconference.org/engine/search/index.php?func=detail&aid=191 (2016-02-01)
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  • Botany & Mycology 2009 - Abstract Search
    Simaroubaceae is an invasive tree from China that has spread over much of North America A number of characteristics contribute to its success including tolerance of poor soils rapid growth rates and production of allelopathic compounds Previous studies indicate increased soil nitrogen at sites invaded by A altissima Increased available soil nitrogen through an alleochemical interaction with legumes may subsidize A altissima s rapid growth on poor soils Nitrogen fixing rhizobia are solicited for nodule development by legumes emitting flavonoids into the rhizosphere under low nitrogen conditions We investigated if and how A altissima root exudates impact nodulation and growth in Trifolium pratense Fabaceae The following treatments were applied to T pratense in a full factorial design soil leachate from soil with or without A altissima leachate source fertilization and T pratense fertilization Treatment effects on T pratense total biomass and shoot root ratio were complex however A altissima leachates increased nodule biomass total biomass ratio and doubled the probability of nodulation regardless of other treatments We are currently monitoring changes in transcript abundance of genes encoding chalcone synthase and chalcone isomerase in T pratense in response to A altissima leachates These genes are involved in the production of the

    Original URL path: http://2009.botanyconference.org/engine/search/index.php?func=detail&aid=876 (2016-02-01)
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  • Botany & Mycology 2009 - Abstract Search
    Section Sanchez Adriana 1 Schuster Tanja M 1 Kron Kathleen A 1 A large scale phylogeny of Polygonaceae based on molecular data Few studies have addressed the evolutionary relationships within Polygonaceae from a global perspective The convoluted taxonomic history of Polygonaceae is a major barrier to understanding evolution in this group and only portions of it have been included in systematic treatments Phylogenetic studies have been limited in both taxon sampling and amount of data Our objective is to identify clades within Polygonaceae and to provide a global estimate of phylogenetic relationships in this morphologically diverse and geographically widespread group We include a total of 75 species representing approximately 40 of the 55 named genera in the family We use three chloroplast regions rbcL matK and ndhF and the ribosomal internal transcribed spacer ITS to understand the phylogenetic relationships in Polygonaceae Maximum Parsimony and Maximum Likelihood are used to analyse the data Symmeria is the sister group to remaining Polygonaceae sampled and there is strong support for this placement Afrobrunnichia branches next but has only moderate support Two large clades comprise Polygonaceae which generally correspond to those found in previous molecular analyses Circumscription of most of the currently recognized subgroups

    Original URL path: http://2009.botanyconference.org/engine/search/index.php?func=detail&aid=53 (2016-02-01)
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  • Botany & Mycology 2009 - Abstract Search
    Acid Catechins and Xanthines from Green Tea Green tea Camellia sinensis a popular beverage enjoyed world wide has been reported to contain compounds used in treatment of cancer Yang et al 2008 genital warts Gross 2008 cardiovascular disease Kuriyama 2008 and as an antimicrobial Wu et al 2007 Accurate measurement and detection of these compounds is necessary for assuring good manufacturing practices and in the conduct of clinical trials As part of a collaborative effort between the National Institute of Standards and Technology NIST and the National Institutes of Health NIH Office of Dietary Supplements analytical methods have been developed for a suite of green tea containing standard reference materials SRMs Previous research suggested and our experience confirmed that the metabolic pool of flavan 3 ols in green tea leaf tissue can be altered by both biotic factors and tea leaf processing Understanding the biology and chemistry of these compounds helped guide the methodological choices Plant primary cell walls are composed of biopolymers cellulose hemicellulose pectin proteins and lignin They also contain irregularly deposited monomeric and polymeric flavan 3 ols Method development for measurement and certification of analytes in green tea leaf powder SRM focused on the monomeric flavan 3

    Original URL path: http://2009.botanyconference.org/engine/search/index.php?func=detail&aid=372 (2016-02-01)
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  • Botany & Mycology 2009 - Abstract Search
    Duncan D 1 All that glitters is not gold and all that s green does not photosynthesise The chlorophyll containing orchid Corallorhiza trifida derives little carbon through photosynthesis While measurements of tissue stable isotope signatures and isotope mixing models have suggested that the green orchid Corallorhiza trifida is photosynthetically active and hence only partially myco heterotrophic these assumptions have not been validated by direct analysis of carbon assimilation The photosynthetic capabilities of three orchid species assumed on the basis of the indirect methods or chlorophyll content to have differing trophic strategies Neottia nidus avis fully myco heterotrophic Cephalanthera damasonium partially autotrophic Corallorhiza partially autotrophic as well as saplings of an autotrophic tree Fagus sylvatica were investigated by combining the determination of chlorophyll content and fluorescence parameters with direct measurement of the potential for CO 2 assimilation using 13 C isotope tracers in the field Chlorophyll content and fluorescence values were indicative of ineffective photochemical processes in Neottia and reduced efficiency of photochemical processes in Corallorhiza These differences are reflected in the mean assimilation rates of 13 CO 2 of 594 129 331 72 12 4 2 4 and 7 3 0 9 mg g 1 h 1 for Fagus Cephalanthera

    Original URL path: http://2009.botanyconference.org/engine/search/index.php?func=detail&aid=22 (2016-02-01)
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