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  • Botany & Plant Biology 2007 - Abstract Search
    37 Unable to connect to database 13 02 37 SQL Statement is null or not a SELECT 13 02 37 Abstract Detail Integrating Plant Systematics Funk Vicki A 1 A key to the tribes of the Compositae One of every 10 flowering plant species is in the family Compositae With ca 24 000 30 000 species in 1700 genera and a distribution that is global except for Antarctica it is the most diverse of all plant families Although clearly monophyletic there is a great deal of diversity among the members Some are well known weeds but most species have restricted distributions and members of this family are often important components of at risk habitats The sub familial classification and ideas about major patterns of evolution and diversification within the family remained largely unchanged from Bentham through Cronquist Recently obtained data both morphological and molecular have allowed us to examine the distribution and evolution of the family and to bring the classification of the family in line with that information There are now ca 10 subfamilies and ca 35 tribes and this makes teaching the family more difficult A preliminary key to the tribes will be presented and it is hoped

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=882 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    Introductory Cell Biology Classes Examining the Antioxidant Enzymatic System and its Responses to Stress The aim of this study is to develop a laboratory exercise that is designed to expose students to a full scale scientific investigation while acquiring science processing skills The project continues over 6 weeks and exposes students to fundamental techniques in cell biology and bioinformatics For the experimental part of their exercise students measure the responses of antioxidant enzymes super oxide dismutase catalase ascorbate peroxidase guaiacol peroxidase and glutathione reductase to abiotic stress For our initial testing of this project we used high light treatment Bioinformatics exercises include comparing DNA and amino acid sequences of each antioxidant enzyme from different species They also use Deep View Swiss PDV Viewer to study the three dimensional structures of the antioxidant enzymes By using this program students are able to analyze several proteins at a time including superimposing structural alignments comparing active sites observing amino acid mutations etc Students present their findings during a classroom mini symposium This learning process is also supported by lecture content The process aims to create and strengthen a student learning community The project can be expanded by running native gels for enzyme activity

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1471 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    vulgaris Establishment of symbiosis between legume plants and nitrogen fixing bacteria is a complex process orchestrated by an exchange of signal molecules developmental and gene expression changes from both organisms During the nodulation process several genes that are essential for the early stages of this symbiotic interaction such as those involved in signal perception and transduction have been identified in legumes Among these genes receptor like kinases RLKs have been found 1 2 which encode for a unique motif with three leucine rich repeats LRR in the extracellular domain a transmembrane helix and an intracellular serine threonine kinase domain Previously in our laboratory a cDNA encoding a LRR RLK was isolated from a cDNA library of P vulgaris roots PvRLK The extracellular domain of PvRLK was cloned and expressed in Escherichia coli as a His tagged protein Herein the immunolocalization of PvRLK with anti PvRLK polyclonal antibodies is showed Data will be presented suggesting that this protein is involved in the vascularization of the nodule Additionally a 3D model of the extracellular domain of the presumed receptor including the LRR domain was done in silico to establish a possible structure function relationship Furthermore biochemical studies are in progress to identify

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=448 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    in Phaseolus vulgaris Establishment of symbiosis between legume plants and nitrogen fixing bacteria is a complex process orchestrated by an exchange of signal molecules developmental and gene expression changes from both organisms During the nodulation process several genes that are essential for the early stages of this symbiotic interaction such as those involved in signal perception and transduction have been identified in legumes Among these genes receptor like kinases RLKs have been found 1 2 which encode for a unique motif with three leucine rich repeats LRR in the extracellular domain a transmembrane helix and an intracellular serine threonine kinase domain Previously in our laboratory a cDNA encoding a LRR RLK was isolated from a cDNA library of P vulgaris roots PvRLK The extracellular domain of PvRLK was cloned and expressed in Escherichia coli as a His tagged protein Herein the immunolocalization of PvRLK with anti PvRLK polyclonal antibodies is showed Data will be presented suggesting that this protein is involved in the vascularization of the nodule Additionally a 3D model of the extracellular domain of the presumed receptor including the LRR domain was done in silico to establish a possible structure function relationship Furthermore biochemical studies are in progress

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=675 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    stress causes large quantities of starch stored in the taproot to be degraded Starch is degraded to maltose moieties by beta amylase The involvement of beta amylase in the break down of starch in alfalfa is still uncertain because of its characteristic accumulation and then disappearance following defoliation The purpose of this research is to purify alfalfa recombinant beta amylase from E coli test for activity as well as the rate of reaction To accomplish this goal the coding region of the alfalfa beta amylase cDNA was amplified by PCR cloned into a PET expression vector and verified for sequence accuracy Protein expression was performed in E coli lDE3 lysogen strain using IPTG to induce the target DNA containing beta amylase Electrophoresis followed by Western blot analysis using sweet potato anti beta amylase to detect alfalfa recombinant beta amylase was used to determine if induction had occurred Alfalfa recombinant beta amylase was purified by affinity chromatography and its activity was tested with the beta amylase specific substrate p nitrophenyl alpha D maltopentaoside PNPG5 containing alpha glucosidase Michealis Menten equation was used to study the kinetics of alfalfa recombinant beta amylase Western blot analysis showed that sweet potato anti beta amylase

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=2729 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    Section Duarte Cristina 1 Fernandes Claudia 2 Romeiras Maria 3 Alien flora of Cape Verde Islands taxonomical biological and biogeographic traits The 10 islands of Cape Verde archipelago are located on the North eastern Atlantic Ocean about 500 km from Senegal in West Africa Cape Verde vascular flora includes about 750 species native and naturalized most of them originated from other geographical regions 30 of the taxa are of uncertain origin As in other insular ecosystems the exotic flora is considered a main threat to native flora and to natural plant communities In this account a taxonomical review of naturalized nonindigenous species is performed Provisional numbers points that from a total of 93 angiosperm families 75 Dicotyledons 18 Monocotyledons 56 have exotic representatives 50 Dicotyledons 7 Monocotyledons The largest families with more than 10 alien species of angiosperms in the alien flora of Cape Verde are Asteraceae Leguminosae including Fabaceae Mimosaceae and Caesalpiniaceae Solanaceae Euphorbiaceae Malvaceae Lamiaceae and Poaceae The genera contributing with more than 3 exotic taxa are the Solanum Amaranthus Chamaesyce Ipomea and Datura Lantana camara and Furcraea foetida are amongst the worst present day invaders in some Cape Verdean habitats whereas other species like Prosopis sp introduced in the archipelago in the last decades appears as a very problematic species in a near future Some traits of naturalized exotic species like grow form herbs shrub trees climbers lianes longevity annual biennial perennials life form Raunkiaer classification and biogeographical origin are analysed and their relationships with species distribution within the archipelago assessed The results will contribute to a first insight of the invasion phenomena in Cape Verde archipelago and aims to be useful in conservation management plans Log in to add this item to your schedule 1 Instituto Investigação Científica Tropical Centro de Botanica Travessa Conde da Ribeira

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1323 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    Joan 3 A little time goes a long way How plant biologists can use short outreach activities to enhance science teaching and learning in K 12 classrooms Many university educators are interested in promoting science education at the K 12 level but finding the time can be challenging From September 2006 to June 2007 we taught elementary school science and developed science curriculum with K 6 teachers as part of University of Minnesota s GK12 program GK12 is a nationally represented National Science Foundation program that seeks to encourage graduate students engagement with the K 12 community through working with educators and other scientists to enhance K 12 STEM science technology engineering and math education Minnesota s program places environmental science graduate students with elementary school teachers at inner city schools in the Twin Cities metro area Most students involved in the program are interested in continuing K 12 outreach after they enter professional careers but realize that their future time to donate to educational outreach may be small We suggest multiple ways that scientists can use short term discrete outreach activities to enhance K 12 teaching and learning We focus on topics and examples from plant biology that we

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1681 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    200 000 secondary metabolites have been elucidated to date In comparison to primary metabolites secondary metabolites are not essential to the growth and development of a plant but rather function in the communication of the plant with its environment The dynamic interactions between competing organisms are reflected in the diversity of secondary metabolites It is presumed that the genes of secondary metabolite formation have been recruited from primary metabolism there is evidence accumulating to support this view Alkaloids in particular appear to function in the chemical defense of the plant as anti feedants and toxins Specifically in our research we investigate how selected plant systems synthesize alkaloids at the enzyme and gene levels The study of the formation of plant alkaloids has greatly advanced in recent years such that a number of genes are now available from S reticuline derived tetrahydrobenzylisoquinoline alkaloid biosynthetic pathways from Berberis spp berberine Eschscholzia californica sanguinarine and Papaver somniferum morphine These species share a central biosynthetic pathway that leads from L tyrosine to S reticuline and most of the cDNAs encoding the enzymes of the central pathway have been identified The biosynthetic pathways branch after S reticuline to form the classes of alkaloids that are

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=749 (2016-02-01)
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