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  • Botany & Plant Biology 2007 - Abstract Search
    to database 13 03 18 Unable to connect to database 13 03 18 SQL Statement is null or not a SELECT 13 03 18 Abstract Detail Genetics Section Zhuang Yan 1 Adams Keith 2 Allelic Variation of Gene Expression in Populus F1 Hybrids Plant hybridization can induce phenotypic novelty and speciation as well as gene expression changes Populus hybrids provide a good system to study interspecific hybridization and its genetic and molecular consequences In this project we determined the allelic variation of gene expression in Populus trichocarpa Populus deltoides F1 hybrids using a single base primer extension assay Among 28 genes analyzed so far 11 showed strong bias at the expression level for one of the two alleles and the expression patterns differed between leaves and stems for five genes These results suggest differential regulation of the two parental alleles in the Populus hybrids To determine if the allelic expression biases were caused by hybridization we compared the ratios of species specific transcripts between hybrids and the parental species Cis regulation was inferred for some genes This research has illuminated aspects of regulation of gene expression upon interspecific hybridization Log in to add this item to your schedule 1 University

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1000 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    Environmental Physiology Lara Núñez Aurora 1 Anaya Ana Luisa 2 Cruz Ortega Rocio 2 Allelochemical stress and abscisic acid in Lycopersicon esculentum Mill Allelochemical stress can be defined as an important mechanism of plant interference mediated by the addition of plant produced secondary products allelochemicals to the soil rhizosphere Our studies have focused in knowing the physiological targets of the phytotoxic compounds released by a noxious and endemic weed Sicyos deppei G Don Cucurbitaceae using as the model of the receptor plant Lycopersicon esculentum Mill Solanaceae since in Mexican crop fields is common to find both plants In this study we wanted to know whether ABA is involved in tomato response to the allelochemicals of S deppei We exposed tomato seeds to S deppei aqueous leachate 0 5 for 3 9 12 24 and 48 h In all times we found that levels of ABA were high in treated compared with control seeds Five to 10 μM ABA applied exogenously had similar effects on germination rate and radicle growth as S deppei treatment To test if the high levels of ABA were related with radicle protusion we analyzed the activity and gene expression of some mycropilar endosperm cell wall degrading enzymes Activity of α galactosidase endo β mananase and β glucanase was decreased corresponding to a down regulation in their expression exo poligalacturonase activity decreased but did not show changes in expression Experiments with diniconazole an inhibitor of ABA catabolism showed that a delay in the degradation of ABA might be involved in allelochemical stress To test if ABA is regulating cell cycle we analyzed gene expression of cycD3 1 and cycB2 1 and CDKB2 1 finding both cyclins down regulated only at 48 h On the contrary CDKB2 1 was down regulated since 3h treatment Currently we are analyzing

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1438 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    Tae 1 Donoghue Michael J 1 Allopolyploid speciation in Persicaria Polygonaceae Persicaria L Mill consists of ca 100 species of weedy herbs found in moist or disturbed areas Phylogenetic analyses were conducted using nuclear ITS and chloroplast DNA partial mat K psb A trn H IGS and trn L F sequences from 60 accessions representing 45 species with an emphasis on section Persicaria Our results revealed a number of conflicts in tree topology between nrITS and cpDNA phylogeny suggesting possible instances of hybrid speciation However we might be underestimating hybridization events due to the concerted evolution in nrITS or overestimating due to the cytoplasmic introgression or lineage sorting To address these possibilities we used the second intron of the nuclear single copy gene LEAFY PL2INT and our sequence analyses provided more direct evidence of hybrid speciation involving allopolyploidy We found distinct copies of PL2INT sequences in polyploid species generally consistent in number with ploidy level The different copies of PL2INT from polyploid species clustered with sequences from the two different diploid species that were hypothesized as parental lineages Furthermore our PL2INT sequence analyses revealed more hybrid speciation events than hypothesized based on incongruence between nrITS and chloroplast gene trees and

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1936 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    Jeremy E 1 Maureira Butler Ivan J 2 Owens Thomas G 3 Doyle Jeff J 3 Allopolyploidy generates novel photosynthesis related phenotypes in wild relatives of soybean Glycine subgenus Glycine Polyploidy is a dominant force in plant evolution and in some cases polyploids are more successful than their diploid progenitors Photosynthesis plays a fundamental role in plant fitness and is a prime example of how polyploids can differ dramatically from their diploid progenitors We are investigating the effects of allopolyploidy on photosynthesis in synthetic and natural allotetraploids of Glycine subgenus Glycine wild relatives of the cultivated soybean G max Using chlorophyll fluorescence and gas exchange methods we show that allopolyploidy has induced a number of novel phenotypes relating to photosynthetic capacity non photochemical quenching capacity NPQ max and the response of quantum yield of photosystem II Φ PSII to chilling stress These data suggest that 1 independent allopolyploid events involving very similar parents can produce a wide range of transgressive phenotypes 2 dramatic changes in photosynthesis can occur immediately after allopolyploidization in synthetic allopolyploids and 3 these changes persist over evolutionary time in natural allopolyploids Because subgenus Glycine includes several related but distinct allopolyploid species we can begin to dissect

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=2029 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    Microtubules are involved in many cellular processes including cell division cell shape intracellular transport signal transduction and plant cell wall formation Microtubules are composed of repeating alpha and beta tubulin subunits encoded by multi gene families with very high overall sequence homology in their protein coding regions but divergent 5 and 3 UTR s There are eight alpha tubulin genes present in the P trichocarpa genome sequence designated PtTUA1 through PtTUA8 The 8 predicted PtTUA proteins show an overall amino acid sequence identity of 88 to 98 but are highly divergent in the last 15 amino acids Twenty beta tubulin genes were identified in silico from the P trichocarpa genome sequence These were designated PtTUB1 through PtTUB20 and their hypothetical proteins range from 89 to 98 sequence identity also with divergent C termini Quantitative real time RT PCR results using 3 UTR specific primers for both PtTUA and PtTUB isotypes showed differential expression of the gene family members across various tissue types PtTUA1 PtTUA5 PtTUB9 and PtTUB15 transcripts are most abundant in developing xylem tissue while PtTUA1 PtTUA6 8 PtTUB7 and PtTUB19 are preferentially expressed in mature pollens The other tissue types showed a more uniform expression pattern of the

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=2733 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    not a SELECT 13 03 43 Abstract Detail Photosynthesis Light Bae Chang Hyu 1 Abe Tomoko 2 Alteration of amounts of plastid genes in a tobacco Nicotiana tobacco L cv BY 4 albino mutant Most of albino mutants have reduced amounts of ptDNA and extensive deletions in their plastid genomes however a few mutants have an increase of plastid genes An albino mutant designated ali was isolated from an M2 population derived from a tobacco Nicotianan tobacco L cv BY 4 plant which had been irradiated with a 14N ion beam at an early stage of embryogenic development Bae et al 2001 Interestingly it is certain that the albino mutant showed increased amounts of plastid genes The amounts of chloroplast genes rbcL psbA 23S rDNA and 16S rDNA whose transcription level was significantly reduced were increased two or three fold in the mutant although the amounts of nuclear gene 25S rDNA were similar in both ali and wild type plants Although cell plan area of albino plant was smaller than those of wild type no significant difference in the numbers of chloroplasts per mesophyll cell was detected Polyploidy level was similar in both ali and wild type plants The results

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1347 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    are essential for plant growth Graminaceous plants release ferric chelating phytosiderophores that bind to Fe and then Fe phytosiderophore complexes across plasma membrane by YELLOW STRIPE 1 YS1 Here we report the characterization of the eighteen rice YSL yellow stripe1 like designated OsYSL genes by examining their expression patterns under the micronutrient deficiency and in various organs To study functional roles of the genes we have identified two independent activation tagged mutants in OsYSL16 from the pool of rice T DNA insertional lines transformed with activation tagging vectors The phenotype of OsYSL16 activation mutants was tested by growing the plants on iron deficient medium Visual differences were reflected by the chlorophyll contents indicating that the OsYSL16 activated mutants are more tolerant to iron deficiency compared to the wild type Iron content of the OsYSL16 activated line in shoots is higher than that of wild type The rate of utilization of limited concentration of iron from the seeds was higher in activation tagging mutants than in wild type Promoter fusions of OsYSL16 to GUS showed that OsYSL16 preferentially expressed in vascular tissues We have also characterized OsYSL15 one of the OsYSL genes that are strongly induced by the iron deficiency Two

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=374 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    cells This polarity is believed to be established and maintained by vesicular delivery and endosomal cycling of proteins in this complex RAB GTPases and their interacting proteins mediate docking of vesicles at specific cellular locations and thereby facilitate targeted protein delivery We have asked if IAA transport IAA efflux proteins and dependent physiological processes are altered in scd1 mutants which have defects in a gene encoding a protein with similarity to RAB binding proteins These mutants were originally isolated for their defect in stomatal cytokinesis but also have profound cell expansion defects in roots and other cell types which is a phenotype frequently linked to auxin response or transport Two alleles were examined with a focus on scd1 1 which is a temperature sensitive mutant allele The roots of scd1 1 are shorter than wild type and have few if any lateral roots when grown at the nonpermissive temperature Gravitropism is also impaired in scd1 1 at the nonpermissive temperature The root phenotypes are also reversible when plants are returned to the permissive temperature Acropetal shoot to root IAA transport a primary source of auxin for root development is also reduced within several hours after transfer to this nonpermissive temperature

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=646 (2016-02-01)
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