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  • Botany & Plant Biology 2007 - Abstract Search
    06 44 SQL Statement is null or not a SELECT 13 06 44 Abstract Detail Non Coding RNAs Kato Tomohiko 1 Asamizu Erika 2 Kaneko Takakazu 2 Tabata Satoshi 2 Hibino Takashi 3 Analysis of micro RNAs in flower development of Eucalyptus Micro RNAs miRNAs are small noncoding RNAs that can play important roles in eukaryotes by mRNA degradation and translation repression To investigate whether miRNAs regulate gene expressions in the flowering process of woody plants we analyzed small RNAs expressed at flowering stage of Eucalyptus First we isolated RNAs from flower buds of Eucalyptus and then separated small RNAs by polyacrylamide gel electrophoresis Twenty two bases of the small RNAs were sequenced by MPSS method and about 120 000 sequence signitures were determined Next the sequences that have homology with rRNA tRNA snRNA scRNA were removed from the 120 000 sequences and then genome sequences including the small RNAs were sereached and about 30 000 sequences were taken Finaly we identified about 500 miRNA candidates by prediction of secondary structures of the 30 000 sequences Some miRNA candisates have sequence conservation in Arabidopsis or Populus but unexpectedly many new miRNA candidates were found in Eucalyptus Several miRNA targets were

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1310 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    2 Olszewski Neil E 2 Analysis of O GlcNAc modification GIGANTEA Protein function can be greatly affected by cytoplasmic nuclear posttranslation modification with O linked N acetylglucosamine O GlcNAc to serine or threonine amino acids Mutant studies with the two arabidopsis O GlcNAc transferases SPY and SEC also indicate that O GlcNAc modification is involved in responses to the hormones gibberellin and cytokinin responses to light circadian regulation meristem gamete and embryo development and viral pathogenesis To determine how modification of individual proteins affects these processes we developed an E coli based co expression system to identify SEC substrates and map the modification sites Plum pox virus PPV capsid protein a protein shown previously to be modified was modified by SEC in E coli with the same specificity as in plants O GlcNAc modification of PPV appears to affect viral pathogenesis We also identified GIGANTEA GI as a SEC substrate and mapped the O GlcNAc modification to a single site threonine 829 GI is a protein that is central to the circadian clock and most GI mutations result in a late flowering phenotype To examine the function of O GlcNAc modification of GI we created transgenic plants that would express wildtype or non modifiable mutant versions of GI under the control of the 35S promoter Although we were able to obtain over 25 independent transformants that were had normal flowering times with the wildtype GI sequence very few plants were obtained that expressed RNA for the non modifiable mutant protein To continue testing O GlcNAc modification of GI we have taken three strategies In case overexpression caused secondary effects new constructs using the GI promoter are under construction Since GI and SPY interact physically and genetically GI might also be modified by SPY To test whether modification by SPY is

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=2113 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    developed several strategies which are able to classify into 1 positive P acquisition from rhizosphere and 2 efficient P recycling in plants OsPI1 Oryza sativa phosphate limitation inducible gene 1 was isolated from rice by microarray analysis as one of the genes responding to P deprivation It has been revealed that OsPI1 codes a non coding RNA and immediately responses when the plant faces to P deprivation OsPI1 is suggested to belong to the TPSI1 Mt4 family which is a P deprivation inducible non coding RNA family And we assumed that OsPI1 has a function of P recycling as well as At4 one of the orthologs isolated from Arabidopsis The aim of this study is to investigate the function of OsPI1 in adaptation on P deprivation Two OsPI1 knockdown lines were constructed by RNAi Growth of knockdown lines under P deprivation decreased in comparison with wild type while P concentration of knockdown lines was still higher This result indicates that OsPI1 expression regulates P recycling system To confirm this idea microarray analysis was conducted to knockdown line and wild type which are grown under P sufficient and deficient conditions Transcriptomic analysis revealed that the increase of mRNA for several genes

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1315 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    to defend themselves by accumulating newly synthesized antifungal compounds called phytoalexins As part of a larger project screening Omani plants for phytoalexin induction we have looked at phytoalexin induction in Nerium oleander of the family apocynaceae Parts of this plant have been used as therapeutic agents against leprosy eye and skin diseases N oleander leaves play a role as anticancer and antibacterial In vitro antimicrobial activity of N oleander roots bark and leaf extracts was studied against bacteria e g Bacillus subtilis Chemical induction of phytoalexins was achieved using CuCl 2 solution and UV induction with wavelength 254 nm Extracted phytoalexins were detected using TLC Cladosporium bioassay followed by large scale preparative TLC Eluted antifungal compounds were purified by TLC and identified by HPLC MS We have identified phytoalexins as well as other constitutive antifungal compounds in Nerium oleander Treated N oleander leaves and solutions accumulated both Pisatin and Genistin which were not detected in control leaves and solutions but not in controls this indicates that these two isoflavonoids are phytoalexins induced antifungal compound in N oleander Another isoflavonoid Pentamethoxyflavone was found as constitutive antifungal compound detected in control solution and in treated leaves The terpenoids Amyrin and Ursolic acid

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1019 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    1 Analysis of Several Traits of Seeds in Micpo endosperm Corn A new type of corn Zea mays L germplasm which only has a little amount of endosperm in fact only have aleurone layer in seeds has been selected since 1994 which named micro endosperm corn abb MEC The ratio of embryo kernel in dry seeds is usually equal to or greater than 30 or even greater than 50 To investigate the value of MEC in science and agricultural production an extensive analysis was performed The results are as follows The single kernel weight of MEC was dispersed from 0 04g to 0 16g The ratio of embryo kernel in weight of dry seeds was about 20 56 to 52 60 The structure of kernel in the microscopy showed that very little endosperm was visible in the maturated kernels of MEC or even invisible endosperm except of aleurone layer The aleurone layers in MEC kernels were thicker than that in the other types of corn such as high oil corn flint corn sweet corn The total content of soluble sugar of kernel in MEC from 18d to 24d after pollination was usually higher than that in super sweet corn respectively The oil content in MEC was about 5 85 to 33 1 in single maturated kernel and 10 13 to 27 62 in corn ears which were higher than that in the other type of corn including the high oil corn The oil content of kernel which the embryo removed in MEC was about 6 21 to 12 92 which were also higher than that in other type of corn To investigate the accumulating of endosperm of seeds in MEC the kernel samples were taken weekly from the 7 days after pollination to date of kernel maturated and were weighted

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=461 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    RNAs Barakat Abdelali 1 Wall P Kerr 2 DiLoreto D Scott 2 dePamphilis Claude W 2 Carlson John 2 Analysis of sRNAome in Populus trichocarpa using pyrosequencing MicroRNAs miRNAs are 20 24 nucleotide small RNAs that negatively control gene expression by cleaving or inhibiting the translation of mRNA of target genes Among miRNA targets are genes involved in plant development and stress defense as well as several genes implicated in different cellular processes microRNAs have been extensively analyzed in rice and Arabidopsis and partially investigated in other plant species So far 109 and 62 miRNA families were identified from Arabidopsis and rice respectively However only 33 miRNAs have been identified from Populus 11 are poplar specific This number is low compared to the number of miRNA families in Arabidopsis and rice indicating that many miRNAs still remain to be discovered The availability of a poplar genome http genome jgi psf org poptrl home html constitutes an opportunity to identify conserved and new poplar specific miRNAs and analyze their diversity in core eudicot and monocot species We analyzed the sRNAome from leaves and vegetative buds of populus using ultrahigh throughput pyrosequencing Two libraries corresponding to the two tissues analyzed were constructed

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=2168 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    07 19 Abstract Detail Protein Modification and Turnover St Pierre Benoit 1 Argueso Cristiana 2 Heim John 3 Matthews Dwight E 3 Delaney Terrence P 4 Analysis of the Arabidopsis thaliana SON1 F Box Protein and Putative Substrates The arabidopsis SON1 gene was discovered as a genetic suppressor mutation that restored disease resistance in systemic acquired resistance SAR deficient nim1 1 plants In that work son1 nim1 1 plants were found to express a novel form of disease resistance and the SON1 gene to encode a putative F box protein Kim and Delaney Plant Cell 14 1469 Subsequent genetic and physiological tests showed that salicylate jasmonate and ethylene signaling pathways were not required for son1 mediated disease resistance Because SON1 is an F box containing protein we predict that it functions as part of an E3 ubiquitin ligase SCF complex to regulate disease resistance To identify candidate SON1 substrate proteins we used GST SON1 fusion proteins to isolate binding proteins from arabidopsis extracts The major protein recovered was purified and rigorously identified using mass spectroscopy analysis The putative substrate protein s interaction with SON1 was confirmed using an in vitro translated epitope tagged version of the candidate protein We are further analyzing this interaction by identifying the respective domains from each protein that are involved in their binding Other tests are underway to assess the biological significance of this interaction and its connection to the disease resistance phenotype of son1 mutant plants In other work SON1 GUS and SON1 mGFP5 transgenic plants have been created to determine the tissue distribution and sub cellular localization of SON1 respectively Together this analysis should provide a fuller understanding of SON1 function and help illuminate its cellular and molecular role s in regulating the novel disease resistance phenotype observed in son1 mutant plants Log

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=396 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    that restored disease resistance in systemic acquired resistance SAR deficient nim1 1 plants In that work son1 nim1 1 plants were found to express a novel form of disease resistance and the SON1 gene to encode a putative F box protein Kim and Delaney Plant Cell 14 1469 Subsequent genetic and physiological tests showed that salicylate jasmonate and ethylene signaling pathways were not required for son1 mediated disease resistance Because SON1 is an F box containing protein we predict that it functions as part of an E3 ubiquitin ligase SCF complex to regulate disease resistance To identify candidate SON1 substrate proteins we used GST SON1 fusion proteins to isolate binding proteins from arabidopsis extracts The major protein recovered was purified and rigorously identified using mass spectroscopy analysis The putative substrate protein s interaction with SON1 was confirmed using an in vitro translated epitope tagged version of the candidate protein We are further analyzing this interaction by identifying the respective domains from each protein that are involved in their binding Other tests are underway to assess the biological significance of this interaction and its connection to the disease resistance phenotype of son1 mutant plants In other work SON1 GUS and SON1

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=703 (2016-02-01)
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