archive-org.com » ORG » B » BOTANYCONFERENCE.ORG

Total: 597

Choose link from "Titles, links and description words view":

Or switch to "Titles and links view".
  • Botany & Plant Biology 2007 - Abstract Search
    Erythronium americanum Erythronium americanum yellow trout lily or dog toothed violet is a spring ephemeral plant inhabiting temperate deciduous forests of the eastern United States Within the species two color forms exist one exhibiting brown purplish anthers and one with yellow anthers Little is known about the genetics of the two color forms including their genetic structure and potential for hybridization One reason for the scarcity of information is the complexity behind the genetic make up of the species Like many other plants E americanum is a polyploid which makes it difficult to use codominant molecular markers such as microsatellites With microsatellite analysis data is interpreted from an electropherogram that represents the different sizes of microsatellite DNA fragments Analysis of electropherograms from diploid organisms is distinct with each allele represented by sets of peaks However when studying organisms with higher ploidy levels elctropherograms are more difficult to elucidate due to variable interpretation of the multiple peaks This research uses a novel approach to microsatellite analysis of polyploidy plants by using the DNA amplified from single grains of pollen By sampling from the gametes it enables easier interpretation of electropherograms by presenting the tetraploids as diploids for analysis The data produced

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=2150 (2016-02-01)
    Open archived version from archive

  • Botany & Plant Biology 2007 - Abstract Search
    J 1 Geri Chiara 2 Laird Janet 3 Yun Byung W 4 Loake Gary 4 Sadanandom Ari 3 Milner Joel J 3 A Novel Defence Suppressor Protein Encoded by Cauliflower Mosaic Virus Inhibits SA Dependent Defence Responses in Arabidopsis and Alters the Localization of NPR1 CaMV stimulates SA dependent responses in pre invasion tissues but these are down regulated in tissues undergoing invasion Here we show that protein P6 the main virus encoded pathogenicity determinant plays a key role in suppressing SA mediated defence In transgenic plants expressing P6 PR 1 BGL2 AOX1A were profoundly unresponsive to SA treatment implying that P6 blocks signaling downstream of SA We also found that following inoculation virulent and avirulent Pseudomonas syringae accumulated to levels in P6 transgenics that were 20 fold higher than in controls and HR was delayed P6 transgenics contained reduced levels of SA but responsiveness to infection by avirulent P syringae was unaffected Surprisingly levels of NPR1 transcripts were elevated by approximately 6 fold in P6 transgenics compared to controls To test whether P6 might be affecting the NPR1 protein we crossed the 35S P6 lines with a line expressing an NPR1 GFP fusion protein As expected in the absence of the P6 transgene GFP was localized in the cytoplasm in untreated leaves but migrated to the nucleus following infiltration with SA However in plants also containing the P6 transgene GFP was always confined to the nucleus even in the absence of SA We have used western blots and immune pulldowns to further investigate the localization of NPR1 GFP in wild type and P6 transgenic backgrounds We find that in the presence of P6 the apparent size of the fusion protein and its nuclear cytoplasmic localization are altered The discovery that a pathogen encoded virulence factor suppresses defence by affecting

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=281 (2016-02-01)
    Open archived version from archive

  • Botany & Plant Biology 2007 - Abstract Search
    Shimada Tomoo 2 Takahashi Hideyuki 2 Ueda Haruko 2 Fukao Yoichiro 3 Kondo Maki 4 Mikio Nishimura 4 Hara Nishimura Ikuko 2 A novel factor MAIGO2 is essential for transport of seed storage proteins from the ER in Arabidopsis thaliana Seed storage proteins are synthesized on the endoplasmic reticulum ER as precursors and then are transported to protein storage vacuoles where they are processed into mature forms We isolated an Arabidopsis mutant maigo2 that accumulated precursors of two major storage proteins 2S albumin and 12S globulin in dry seeds 1 mag2 seed cells contained many novel structures with an electron dense core composed of the precursors of 2S albumin 12S globulins were localized in the matrix region of the structures together with ER chaperones BiP and PDI which were more abundant in mag2 seeds MAG2 protein had a RINT 1 TIP20 domain in the C terminal region Some MAG2 molecules were peripherally associated with the ER membrane MAG2 had an ability to bind to two ER localized t SNAREs At Sec20 and At Ufe1 Our findings suggest that MAG2 functions in the transport of storage protein precursors between the ER and the Golgi complex in plants 1 Li et al

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=810 (2016-02-01)
    Open archived version from archive

  • Botany & Plant Biology 2007 - Abstract Search
    SELECT 13 11 12 Abstract Detail Evolution of Development and Physiology Han Yuepeng 1 Korban Schuyler 2 A novel family of miniature inverted repeat transposable elements is associated with genes in apple The apple Malus domestica Borkh belongs to the family Rosaceae and subfamily Maloideae and has a genome size of 750 Mb Here we report on a novel family of transposable elements designated Spring found in the apple genome Four Spring elements Spring 1 to Spring 4 share all classic features of miniature inverted repeat transposable elements MITEs including small size 148 bp lack of coding potential A T richness insertion bias toward noncoding regions terminal inverted repeats TIRs target site duplications TSDs and potential for forming secondary structures Previous mobility of Spring 4 is demonstrated by sequence alignment of genes encoding 1 aminocyclopropane 1 carboxylic acid ACC synthase from both apple and a related member of the Maloideae subfamily pear It is observed that Spring elements are flanked by either 7 or 8 bp direct repeats In addition these Spring elements in the apple genome significantly differ in size 2 or 3 bp from those MITEs previously reported in various plant speices In fact the TIRs of these

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1913 (2016-02-01)
    Open archived version from archive

  • Botany & Plant Biology 2007 - Abstract Search
    2 Cook Douglas R 3 Ane Jean Michel 4 A novel nuclear protein interacts with the symbiotic DMI3 CCaMK of Medicago truncatula Most higher plants are able to develop symbiotic associations with arbuscular mycorrhizal AM fungi that facilitate the uptake of inorganic nutrients from the soil and protects them against biotic and abiotic stresses Legumes can also enter into a mutualism with nitrogen fixing bacteria named rhizobia Several genes that control such interactions have been cloned in model legumes using forward genetic approaches DMI3 Does Not Make Infections 3 encodes a calcium calmodulin dependent kinase CCaMK that is required for the establishment of both legume nodulation and AM symbiosis We are interested in identifying proteins that interact with DMI3 either as controlling or as targets of its kinase activity We employed a yeast two hybrid approach and identified a novel protein that interacts with DMI3 named IPD3 Interacting Protein of DMI3 IPD3 is predicted to interact with DMI3 through a C terminal coiled coil domain Like DMI3 this protein was shown to be localized to the nucleus of Medicago truncatula root cells Split yellow fluorescent protein YFP assays suggest that DMI3 interacts physically with IPD3 in the nuclear compartment Promoter

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=247 (2016-02-01)
    Open archived version from archive

  • Botany & Plant Biology 2007 - Abstract Search
    Arabidopsis Leaves of flowering plants develop with a distinct adaxial abaxial asymmetry that reflects functional specializations for intercepting light energy and exchanging gases Microsurgical and molecular genetic studies have demonstrated that the proper specification of adaxial abaxial polarity requires communication between the SAM and the incipient leaf and that subsequent blade outgrowth is dependent on the juxtaposition of adaxial and abaxial fates Adaxial abaxial asymmetry in lateral organs depends on mutually repressive interactions between members of several families of transcription factors and on posttranscriptional regulation by small regulatory RNAs reviewed by Kidner 2007 We have recently characterized a new regulator of leaf polarity FLAVODENTATA FLV which reveals a previously unidentified relationship between chloroplast function and adaxial abaxial polarity during leaf development The flv mutant shows light intensity dependent defects in greening and leaf morphogenesis Young leaves on flv plants are serrated white to pale green and occasionally form radially symmetric needle shaped leaves The reduced greening observed in flv suggests defects in chloroplast function whereas needle shaped leaves indicate defects in the proper specification of adaxial leaf fate We found that the FLV encodes a pentatricopeptide repeat PPR protein with a predicted chloroplast signal sequence Members of the PPR family

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=2677 (2016-02-01)
    Open archived version from archive

  • Botany & Plant Biology 2007 - Abstract Search
    and other aspects of mRNA metabolism We have previously isolated a unique plant SR protein SR45 with atypical domain organization Unlike typical SR proteins SR 45 contains two RS domains one on either side of the RNA recognition motif However the biological and molecular functions of this novel SR protein are not known Using an in vitro splicing complementation assay we showed that SR45 functions as an essential splicing factor A T DNA insertion mutant sr45 1 exhibited developmental abnormalities including altered number of petals and stamens and delayed flowering time The late flowering phenotype was observed under both long days and short days and was rescued by vernalization Expression analysis of several flowering time genes using RT PCR and microarrays revealed that FLC a key flowering repressor is up regulated in sr45 1 demonstrating that SR45 influences the autonomous flowering pathway Furthermore the alternative splicing patterns of several other SR genes was altered in sr45 1 in a tissue specific manner Hence the observed phenotypic abnormalities are likely due to altered levels of SR protein isoforms which in turn modulate splicing of other pre mRNAs Changes in the alternative splicing of SR genes and the flowering time in the

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=681 (2016-02-01)
    Open archived version from archive

  • Botany & Plant Biology 2007 - Abstract Search
    41 Abstract Detail Reproductive Development Ali Gul 1 Palusa Saiprasad 2 Golovkin Maxim 3 Prasad Jayendra 4 Manley James 5 Reddy Anireddy S N 2 A novel plant specific splicing factor regulates Flowering in Arabidopsis Serine arginine rich SR proteins play important roles in constitutive and alternative splicing and other aspects of mRNA metabolism We have previously isolated a unique plant SR protein SR45 with atypical domain organization Unlike typical SR proteins SR 45 contains two RS domains one on either side of the RNA recognition motif However the biological and molecular functions of this novel SR protein are not known Using an in vitro splicing complementation assay we showed that SR45 functions as an essential splicing factor A T DNA insertion mutant sr45 1 exhibited developmental abnormalities including altered number of petals and stamens and delayed flowering time The late flowering phenotype was observed under both long days and short days and was rescued by vernalization Expression analysis of several flowering time genes using RT PCR and microarrays revealed that FLC a key flowering repressor is up regulated in sr45 1 demonstrating that SR45 influences the autonomous flowering pathway Furthermore the alternative splicing patterns of several other SR genes was altered in sr45 1 in a tissue specific manner Hence the observed phenotypic abnormalities are likely due to altered levels of SR protein isoforms which in turn modulate splicing of other pre mRNAs Changes in the alternative splicing of SR genes and the flowering time in the mutant were rescued by expressing full length SR45 cDNA further confirming that the observed defects in the mutant are due to the lack of SR45 These results indicate that SR45 is a novel plant specific splicing factor that plays a crucial role in flowering Log in to add this item to your

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=535 (2016-02-01)
    Open archived version from archive