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  • Botany & Plant Biology 2007 - Abstract Search
    Accumulation Ca 2 binding proteins encoded in the Arabidopsis genome are predicted to mediate cellular responses to Ca 2 fluctuations In the present work we find two related putative Ca 2 sensors CML23 and CML24 regulate the transition to flowering The cml24 4 allele behaves as a loss of function and causes late flowering specifically under long days indicating the CML24 is required for proper functioning of the photoperiod pathway Conversely cml24 2 with a substitution of a conserved amino acid in an EF hand Ca 2 binding motif flowers early Opposing phenotypes suggest that CML24 can act as a switch in the photoperiod pathway regulating flowering transition Based on quantitative RT PCR we place CML24 function upstream of Constans CO cml24 4 has reduced CO expression cml24 2 has elevated CO expression Mutants harboring loss of function alleles of both CML24 and the closely related paralog CML23 are also defective in the autonomous pathway regulating flowering transition cml23 cml24 double mutants have elevated Flowering Locus C FLC expression and flower late under all photoperiods Both single and double mutants respond normally to gibberellin and vernalization These roles for CML23 and CML24 in regulating flowering transition may link circadian and

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=667 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    Biology Frank Aubrey C 1 von Besser Kiera 2 Wong Julian L 1 Chaibang Adisorn 1 Heyward Keith 1 Preuss Daphne 2 Johnson Mark A 3 Arabidopsis HAP2 GCS1 is sperm specific required for pollen tube guidance and essential for fertilization Double fertilization occurs after two sperm are delivered to the female gametophyte by a pollen tube one sperm fuses with the egg to produce the embryo and the other fuses with the central cell to produce the endosperm The molecules responsible for gamete interaction and initiation of seed production are unknown We identified the Arabidopsis hap2 mutant in a genetic screen for pollen expressed genes that are critical for pollen development and function hap2 mutant sperm are delivered to the female gametophyte by hap2 pollen tubes but they are unable to fertilize either the egg or the central cell Analysis of the hap2 mutant and the HAP2 gene offer a unique opportunity to gain insight into the molecules that mediate fertilization in angiosperms HAP2 is a novel transmembrane protein that is likely conserved among all plants We will discuss experiments designed to test the hypothesis that HAP2 interacts with proteins expressed by the egg and central cell and that

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=654 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    Aubrey C 1 von Besser Kiera 2 Wong Julian L 1 Chaibang Adisorn 1 Heyward Keith 1 Preuss Daphne 2 Johnson Mark A 3 Arabidopsis HAP2 GCS1 is sperm specific required for pollen tube guidance and essential for fertilization Double fertilization occurs after two sperm are delivered to the female gametophyte by a pollen tube one sperm fuses with the egg to produce the embryo and the other fuses with the central cell to produce the endosperm The molecules responsible for gamete interaction and initiation of seed production are unknown We identified the Arabidopsis hap2 mutant in a genetic screen for pollen expressed genes that are critical for pollen development and function hap2 mutant sperm are delivered to the female gametophyte by hap2 pollen tubes but they are unable to fertilize either the egg or the central cell Analysis of the hap2 mutant and the HAP2 gene offer a unique opportunity to gain insight into the molecules that mediate fertilization in angiosperms HAP2 is a novel transmembrane protein that is likely conserved among all plants We will discuss experiments designed to test the hypothesis that HAP2 interacts with proteins expressed by the egg and central cell and that these interactions

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=496 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    Unable to connect to database 13 17 11 SQL Statement is null or not a SELECT 13 17 11 Abstract Detail Salinity Chen Zhen 1 Li Yan 1 Xia Huijun 1 Arabidopsis Inositol Polyphosphate 6 3 Kinase Gene AtIpk2ß Enhances Salt Resistance via Ionic and ABA Pathways Abiotic stresses especially salt severely affect plant productivity Adverse effect of salinity on plant growth is mainly due to ion toxicity and osmotic stress We previously identified an inositol polyphosphate 6 3 kinase gene AtIpk2ß from Arabidopsis However a little is known about its physiological functions in higher plants Here we report that AtIpk2ß enhances salt resistance via ionic and ABA pathways AtIpk2ß was induced by NaCl and ABA Using root bending assay we found its T DNA insertion line exhibited increased sensitivity to NaCl Meanwhile AtIpk2ß overexpression lines were resistant to NaCl Furthermore the mutant lines were also sensitive to some ions We also found that the stomata of mutant lines were insensitive to ABA induced closing However the overexpression lines displayed opposite phenotypes to the mutant RT PCR experiments showed increased expression of some stress responsive genes in the mutant lines and decreased expression in overexprssion lines Further studies need to

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1621 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    connect to database 13 17 16 SQL Statement is null or not a SELECT 13 17 16 Abstract Detail Reproductive Development Kanrar Siddhartha 1 Becerra Benjamin 2 Smith Harley 2 Arabidopsis KNOX BELL proteins regulate flower specification in Arabidopsis In flowering plants post embryonic development is regulated by meristems The shoot apical meristem SAM initiates primordial including leaves internodes and axillary meristems repetitively from its flanks Axillary meristems can develop into secondary shoots or flowers In Arabidopsis two paralogous BEL1 like BELL homeobox genes PENNYWISE PNY and POUNDFOOLISH PNF are expressed in morphogenic region of the SAM These homeobox genes encode DNA binding proteins that are essential for specifying floral primordial and establishing early internode patterning events during inflorescence development Biochemical studies showed that PNY and PNF associate with the knotted1 like homeobox KNOX protein SHOOT MERISTEMLESS STM and regulate inflorescence architecture during reproductive development We are utilizing proteomic genetic and molecular approaches to understand the molecular mechanisms of PNY PNF STM heterodimers and their role in floral specification Results generated from these studies will be presented in the symposium Log in to add this item to your schedule 1 Center for Plant Cell Biology Institute for Integrative Genome Biolog

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=390 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    1 Armstrong Greg 2 Arabidopsis light independent protochlorophyllide oxidoreductase A PORA restores bulk chlorophyll synthesis to a porB porC double mutant In angiosperms the strictly light dependent reduction of protochlorophyllide Pchlide to chlorophyllide is catalyzed by NADPH Pchlide oxidoreductase POR The Arabidopsis thaliana genome encodes three structurally related but differentially regulated POR genes PORA PORB and PORC PORA and PORB are coexpressed strongly early in development during etiolation germination and greening at the seedling cotyledon stage PORB and PORC are coexpressed both during seedling development and throughout the later life of the plant and therefore are responsible for post cotyledon bulk chlorophyll synthesis The Arabidopsis porB 1 porC 1 double mutant displays a xantha phenotype and has both reduced amounts of prolamellar bodies and thylakoid membrane stacking in its plastids Constitutive overexpression of PORA in the double mutant background produces slightly larger prolamellar bodies in etioplasts Furthermore bulk chlorophyll synthesis and thylakoid stacking are restored in the light grown PORA overexpressing porB porC double mutant Chlorophyll production during seedling greening after prior etiolation is also restored to normal levels in the PORA rescued plants An Arabidopsis porB porC double mutant can therefore be functionally rescued by the addition of the

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=933 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    3 Bressan Ray A 3 Arabidopsis locus Resurrection rst Controls Susceptibility to Powdery Mildew Plant growth and development is continuously modified by biotic and abiotic factors in the environment Many biotic stresses can affect the yield of agricultural crops The mechanism of plant response to pathogen attack is complex and there are still unknown factors affecting plant defense response Our objective is to better understand plant defense mechanism to pathogenic fungi attack through a molecular and genetic approach We recently isolated a novel Arabidopsis mutant called resurrection rst which shows reduced stem inflorescence and visual glaucousness due to altered cuticular wax structure and composition Interestingly rst mutants were disease susceptible to the obligate biotrophic powdery mildew We hypothesize infected rst mutants interacting with various pathogens will provide information to better understand plant defense responses to pathogen invasion We infected rst mutant plants with obligate biotrophic powdery mildew Erysiphe cichoracearum and necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola Preliminary results indicate that rst plants were more susceptible to Erysiphe cichoracearum however showed greater resistance to Botrytis cinerea and Alternaria brassicicola than wild type plants The enhanced fungal growth of Erysiphe cichoracearum developed more rapidly on the rst mutant producing conidia

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1958 (2016-02-01)
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  • Botany & Plant Biology 2007 - Abstract Search
    Ansell Stephen 1 Grundmann Michael 1 Schneider Harald 2 Russell Stephen J 1 Vogel Johannes C 1 Arabidopsis lyrata ssp petraea Non model patterns of glacial history in a model plant Arabidopsis lyrata spp petraea Arabidopsis lyrata a close relative to A thaliana is an emerging model organism for evolutionary and ecological genetics of plants Climate oscillations during Quaternary have caused dramatic geographic structuring of variation in this plant due to range fragmentation genetic drift and recolonisation With no fossil remains and no extant populations in putative southern European glacial refugia the lack of a phylogeographic hypothesis is hindering the study of selection and adaptation in natural populations Populations from its entire and disjunct European range were studied for nuclear and cpDNA diversity All populations were highly genetically variable but geographic location and genetic diversity did not correlate Population differentiation between central and northern Europe areas was significant but differentiation is only moderate within regions Chloroplast DNA confirmed the existence of two gene pools and identified population admixture around the southern edges of northern distribution ranges Thus during the last glaciation the small perennial plant survived in two refugia between the Nordic and Alpine glaciers one cryptic one in central

    Original URL path: http://2007.botanyconference.org/engine/search/index.php?func=detail&aid=1583 (2016-02-01)
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