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  • EMMA - the European Mouse Mutant Archive
    on the order link for the appropriate strain you will be guided to a request form which you can quickly fill in and send to EMMA through the website The request form will ask you for contact information and whether you wish to receive frozen sperm or embryos or live animals After receipt of your request EMMA will contact you within one week What can I obtain and what is the delivery time You can obtain sperm or embryos as frozen samples with a delivery time of four weeks If you order live mice after rederivation EMMA will provide up to three breeding pairs per mouse strain within four to six months of the request However some strains in high demand are maintained as live colonies and can be shipped within one month Some strains require special expertise to be reliably rederived from frozen samples Therefore EMMA only distributes rederived mice in these cases Whether a strain is held as frozen material or live mice on shelf is displayed on the respective strain information page of the EMMA website under Availability As the default heterozygous material is being archived and distributed Can I register interest for strains that are still under development EMMA has newly submitted strains in various stages of preservation development and colony establishment These strains are not yet available for distribution and can not be ordered yet You should however indicate your interest in obtaining them The EMMA Strain Interest Registration Form is accessible via the register interest link on the EMMA strain list and on the strain description page This registration of interest is valuable in helping EMMA to anticipate demand and to optimize availability of strains Customers will receive notice as soon as the strain becomes available and be able to place their strain request in time What does EMMA charge Standard fees Shipments of frozen embryos sperm cost 1100 while live mice are distributed for a cost of 2400 The 2400 covers the provision of a breeding nucleus of up to three pairs of SPF mice The charges levied by EMMA are used solely to meet the animal husbandry and stock replacement costs Customers are still asked to meet the actual costs for shipping the material from the EMMA facility to the recipient s institution As the default heterozygous material is being archived and distributed EMMA does not generally offer breeding services to e g generate cohorts of homozygous mutants However such a service and fees may be negotiated with the respective EMMA distribution centre In general service requests will not be processed until a fully executed Material Transfer Agreement MTA has been received where applicable and either a billing address or a purchase order number has been provided by the customer In case of cancellations of service requests EMMA reserves the right to charge customers for incurred costs The individual EMMA repositories will each bill customers separately How many embryos will I receive EMMA will ship two straws containing approximately 25 embryos straw

    Original URL path: http://strains.emmanet.org/faqs.php (2016-02-17)
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  • EMMA - the European Mouse Mutant Archive
    use validated protocols for mouse embryo and spermatozoa freezing techniques in vitro fertilisation procedures and embryo transfers The protocols provided for download on this website are practiced in EMMA training courses at the MRC MGU Harwell and the CNRS CDTA Orléans and are routinely used at Harwell and Orléans The EMMA short training courses are intended to give animal technologists practical hands on experience of the murine embryo and spermatozoa freezing techniques simple and robust in vitro fertilization procedures and frozen embryo resuscitations The protocol on the production of germ free mice is provided by the Gulbenkian Institute which is offering the EMMA axenic germ free service In addition to the validated protocols listed here EMMA distribution centres will provide to customers ordering frozen embryos the centre specific validated thawing protocols Introductory presentation on cryopreservation download PDF copy 1 3 MB Abstracts and presentations about cryopreservation techniques from the EMMA cryopreservation workshop held in May 2012 in Madrid Workshop website at CSIC Mouse sperm cryopreservation and IVF recovery MBCD GSH protocol Feb 2013 download PDF copy of protocol Mouse IVF recovery using freshly harvested sperm MBCD GSH protocol Feb 2013 download PDF copy of protocol Rescue protocol for frozen sperm

    Original URL path: http://strains.emmanet.org/protocols.php (2016-02-17)
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  • EMMA - the European Mouse Mutant Archive
    CSIC Consejo Superior de Investigaciones Científicas main campus It emerged as an initiative from the EMMA technology development working group and was organized by EMMA and the CSIC At the workshop an extraordinary group of experts in the cryopreservation field brainstormed and discussed in depth the latest technological advances in cryopreservation including sperm and embryo cryopreservation updated in vitro fertilization IVF methods and related techniques as ovary cryopreservation laser assisted and piezo driven intracytoplasmic sperm injection ICSI transportation of frozen material and other technical and logistic challenges relevant to the operation of current mouse embryo sperm archives The workshop was funded by the EC s FP7 Capacities Specific Programme EMMAservice project and co sponsored by the ISTT International Society for Transgenic Technologies Program and abstracts presented at this workshop pdf file 588 KB Workshop website at CSIC with presentations for download Organized by EMMA and CSIC through Martin Fray Mary Lyon Centre MRC Harwell UK Michael Hagn Institute of Experimental Genetics HMGU Munich Germany Lluís Montoliu National Center of Biotechnology CNB CSIC Madrid Spain List of invited speakers Sue Bath Melbourne Australia Martina Crispo Institut Pasteur Montevideo Uruguay Xiang Gao Model Animal Research Center Nanjing University Nanjing P R China Marina Gertsenstein Toronto Centre for Phenogenomics Toronto ON Canada Carlisle Landel Transposagen Biopharmaceuticals Inc Lexington KY USA Kent Lloyd Mouse Biology Program University of California Davis CA USA Peter Mazur The University of Tennessee Knoxville TN USA Keiji Mochida RIKEN Bioresource Center Tsukuba shi Ibaraki Japan Naomi Nakagata Center for Animal Resources Development CARD Kumamoto University Kumamoto Japan Lauryl Nutter Toronto Centre for Phenogenomics Toronto ON Canada Atsuo Ogura RIKEN Bioresource Center Tsukuba shi Ibaraki Japan Marcello Raspa EMMA CNR Monterotondo Rome Italy Stuart Read The Australian National University The Australian Phenomics Facility Canberra Australia Jorge Sztein CMB Cryopreservation and

    Original URL path: http://strains.emmanet.org/workshop.php (2016-02-17)
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  • EMMA - the European Mouse Mutant Archive
    Harwell Mouse Embryo and Spermatozoa Cryopreservation Training Course Course outline and agenda 2011 17 21th October 2011 CNR The Jackson Lab EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm further information download the application form doc file 328 kB 12 15th September 2011 MRC Harwell Mouse Embryo and Spermatozoa Cryopreservation Training Course Course outline and agenda 28 30th March 2011 CNRS TAAM Orléans Course on Rederivation Techniques Formation aux Techniques de Rederivation download course description English French download application form English French 28th February 3rd March 2011 MRC Harwell Mouse Embryo and Spermatozoa Cryopreservation Training Course Course agenda 2010 25 29th October 2010 CNR The Jackson Lab EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm further information download the application form doc file 328 kB 4 6th Oct 2010 CNRS TAAM Orléans Course on Rederivation Techniques Formation aux Techniques de Rederivation download course description English French download application form English French 6 9th September 2010 MRC Harwell Mouse Embryo and Spermatozoa Cryopreservation Training Course Course agenda 22nd 25th February 2010 MRC Harwell Mouse Embryo and Spermatozoa Cryopreservation Training Course Course agenda 25 27th Jan 2010 CNRS TAAM Orléans Course on Rederivation Techniques Formation aux Techniques de Rederivation download course description English French download application form English French 2009 23 26th Nov 2009 CNR The Jackson Lab EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm see the poster 24 27th August 2009 MRC Harwell Mouse Embryo and Spermatozoa Cryopreservation Training Course Course agenda 16 19th February 2009 MRC Harwell Mouse Embryo and Spermatozoa Cryopreservation Training Course Course agenda 2008 27 30th Oct 2008 CNR The Jackson Lab EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm see the poster 11 14th August 2008 MRC Harwell Mouse Embryo and Spermatozoa Cryopreservation Training Course Course agenda 11 14th February 2008 MRC Harwell Mouse Embryo

    Original URL path: http://strains.emmanet.org/courses.php (2016-02-17)
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  • EMMA - the European Mouse Mutant Archive
    Cryopreservation general Freezing of mouse embryos Vitrification Freezing of mouse spermatozoa ICSI Freezing of oocytes Freezing of ovaries Gnotobiology Health monitoring Links Internal Site Bibliography Overview Selected reading cryopreservation techniques EMMA has collected publications covering different aspects of cryopreservation techniques The EMMA partners find these articles and reviews useful for scientists who want to apply archiving techniques The lists are not exhaustive Cryopreservation general Freezing of mouse embryos Freezing of

    Original URL path: http://strains.emmanet.org/bibliography/overview.php (2016-02-17)
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  • EMMA - the European Mouse Mutant Archive
    Yamazaki Y McMahan CA Walter CA Yanagimachi R et al Assisted reproductive technologies do not alter mutation frequency or spectrum Proc Natl Acad Sci U S A 2007 104 5085 90 PMID 17360354 Crabbe JC Schneider U Hall JW Mazur P Invited commentary cryopreservation as a tool for the study of selectively bred lines in rodent behavioral genetics Behav Genet 1993 23 307 12 PMID 8240209 Fahning ML Garcia MA Status of cryopreservation of embryos from domestic animals Cryobiology 1992 29 1 18 PMID 1606826 Fray M D 2009 Biological methods for archiving and maintaining mutant laboratory mice Part I conserving mutant strains Methods Mol Biol 561 301 319 PMID 19504080 Fray M D 2009 Biological methods for archiving and maintaining mutant laboratory mice Part II recovery and distribution of conserved mutant strains Methods Mol Biol 561 321 332 PMID 19504081 Fray M D A R Pickard M Harrison and M T Cheeseman 2008 Upgrading mouse health and welfare direct benefits of a large scale rederivation programme Lab Anim 42 127 139 PMID 18435871 Glenister PH Thornton CE Cryoconservation archiving for the future Mamm Genome 2000 11 565 71 PMID 10886024 Glenister PH Whittingham DG Wood MJ Genome cryopreservation a valuable contribution to mammalian genetic research Genet Res 1990 56 253 8 PMID 2272516 Guan M Marschall S Raspa M Pickard AR and Fray MD Overview of new developments in and the future of cryopreservation in the laboratory mouse Mamm Genome 2012 23 572 9 PMID 22936001 Kasai M Cryopreservation of mammalian embryos Mol Biotechnol 1997 7 173 9 PMID 9219232 Landel CP Archiving mouse strains by cryopreservation Lab Anim NY 2005 34 50 7 PMID 15806091 Liebermann J Nawroth F Isachenko V Isachenko E Rahimi G Tucker MJ Potential importance of vitrification in reproductive medicine Biol Reprod 2002 67

    Original URL path: http://strains.emmanet.org/bibliography/general.php (2016-02-17)
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  • EMMA - the European Mouse Mutant Archive
    Bibliography Cryopreservation general Freezing of mouse embryos Vitrification Freezing of mouse spermatozoa ICSI Freezing of oocytes Freezing of ovaries Gnotobiology Health monitoring Links Internal Site Bibliography Freezing of mouse embryos Key references Byers SL Payson SJ Taft RA Performance of ten inbred mouse strains following assisted reproductive technologies ARTs Theriogenology 2006 65 1716 26 PMID 16271754 Renard JP Babinet C High survival of mouse embryos after rapid freezing and thawing

    Original URL path: http://strains.emmanet.org/bibliography/mouse-embryo-freezing.php (2016-02-17)
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  • EMMA - the European Mouse Mutant Archive
    of oocytes Freezing of ovaries Gnotobiology Health monitoring Links Internal Site Bibliography Freezing of mouse embryos by vitrification Key references Kasai M Cryopreservation of mammalian embryos Vitrification Methods Mol Biol 1995 38 211 9 PMID 7647860 Kasai M Komi JH Takakamo A Tsudera H Sakurai T Machida T A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution without appreciable loss of viability J Reprod Fertil 1990

    Original URL path: http://strains.emmanet.org/bibliography/mouse-embryo-vitrification.php (2016-02-17)
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