archive-org.com » ORG » I » IMGS.ORG

Total: 854

Choose link from "Titles, links and description words view":

Or switch to "Titles and links view".
  • The 16th International Mouse Genome Conference (2002)
    Attendees Sponsors Table of Contents Photographs Awards POSTER 92 INDUCIBLE GENE TRAPPING USING DRUG SELECTABLE MARKERS AND THE CRE LOXP SYSTEM TO IDENTIFY DEVELOPMENTALLY REGULATED GENES YT Chen Baylor College of Medicine 1 Liu P 1 Vaishishnav S 2 Bradley A 1 Baylor College of Medicine 2 The Welcome Trust Sanger Institute Gene trapping in mouse ES cells is an important functional genomics approach It allows identification of novel genes and the generation of corresponding mutant mice at the same time To develop a system which allows in vitro prescreening of gene traps that respond to signaling molecules of interest we designed a selection scheme using drug selectable markers and the Cre loxP technology to perform in vitro prescreening in a 96 well format In our experiment 920 gene trap clones were assayed and 258 of them were classified as gene traps induced by in vitro differentiation Sixty five of the in vitro differentiation inducible gene traps were also responsive to retinoic acid RA treatment An analysis of 13 RA inducible gene trap clones in vivo reveals that nearly 85 11 out of 13 of the RA inducible gene traps trapped developmentally regulated genes This result is consistent with the

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file92.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    EBFAZ RELATED KRÜPPEL LIKE ZINC FINGER GENE S Warming National Cancer Institute Liu P Suzuki T Akagi K Lindtner S Pavlakis G Jenkins N Copeland N National Cancer Institute Evi3 is a common site of retroviral integration in B cell lymphomas of AKXD mice BLAST searches of Evi3 genomic sequences against the mouse and human databases showed that most viral integrations at Evi3 are located immediately upstream of the first translated exon exon 2 of a gene encoding a novel zinc finger protein with 30 krüppel like zinc finger repeats Viral integrations at Evi3 upregulate the expression of this gene via promoter sequences present in the viral long terminal repeat EVI3 protein is highly related to the early B cell associated zinc finger protein EBFAZ and all 30 zinc fingers found in EVI3 are conserved in EBFAZ Ebfaz and Evi3 are coexpressed in many cell types and EVI3 like EBFAZ is located in the nucleus EBFAZ binds to and negatively regulates early B cell factor EBF a basic helix loop helix transcription factor required for B cell lineage commitment EBFAZ also binds to SMAD1 and SMAD4 in response to BMP2 signaling which in turn activates the homeobox regulator of Xenopus

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file93.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    Contents Photographs Awards POSTER 94 UPDATE IN THE USE OF SPEED BACKCROSSES FOR MAPPING HARWELL ENU MUTATIONS P H Glenister Medical Research Council Fray M Woodward A Brown S MRC Many novel mutations have been recovered from the Harwell mutagenesis programme and a number of these are potential models for human genetic disease The initial phase in studying an interesting phenotype that may be a putative human model involves linking the mutation to the genetic map of the mouse When a map position is found comparison of the human and mouse homologous regions may indicate a prospective candidate gene Before the mutant gene can be cloned fine genetic mapping is required All these procedures call for a large number of backcross progeny Producing these numbers by conventional breeding is time consuming or may be impossible due to deleterious effects of a particular mutation Using in vitro fertilisation we have produced enough animals to provide an initial map position from a single IVF session Apart from the saving in time there is a valuable saving in animal house space as embryo recipient females are housed in groups until just before parturition A further advantage is that the backcross animals are born

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file94.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    Noda T 2 Shiroishi T 1 Gondo Y 1 Population and Quantitative Genomics Team and 2 Mouse Functional Genomics Research Group RIKEN Genomic Sciences Center Many inheritable mutants have been isolated by dominant screening in RIKEN ENU mutagenesis http www gsc riken go jp Mouse In the mutagenesis project it is crucial to identify the site of mutations in the genome for the mutants To this purpose we are currently taking three major approaches Positional cloning ENU mutagenized C57BL 6 males are mated to DBA 2 females to produce G1 for the phenotype driven approach Phenodeviant G1 are subjected to inheritance test in G2 progeny that also provide linkage for the rough mapping The sites of mutations will be identified by fine mapping and positional cloning or candidate gene approach as described below Candidate gene approach It is now plausible to list up candidate genes around the rough mapped region due to the substantial public database of mouse and or human genome We are now designing PCR primers for direct sequencing of candidate genes of some mutants We have found three possible causable point mutations in Pax 6 gene The primers that are prepared for the candidate gene approach are

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file95.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    Kemp S 1 Iraqi F 1 International Livestock Research Institute 2 Jomo Kenyatta University of Agriculture and Technology 3 School of Biological Sciences University of Liverpool Trypanosomosis resistance Trypanotolerance is exhibited by different inbred strains of mice after T congolense challenge In a previous study trypanotolerance quantitative trait loci QTL was mapped to chromosomes 17 5 and 1 using two F2 resource populations C57BL 6 x A J and C57BL 6 x BALB c and designated as Tir1 Tir2 and Tir3 respectively Using two F6 advanced intercross line AIL populations Tir1 was mapped to less than 1cM while Tir2 and 3 were mapped to large genomic intervals In order to improve the resolution of Tir2 and 3 and identify possible candidate genes F12 C57BL 6J x A J AIL fixed for the susceptible and resistance alleles at Tir1 locus were generated The two AIL populations homozygous for the resistant and susceptible Tir1 alleles together with the parental controls were challenged with T congolense and followed for survival times over 180 days Mice from the two survival extremes of the selected populations were genotyped with a panel of microsatellite markers across the previously mapped region and the data analysed with the

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file98.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    Consortium Oak Ridge National Laboratory The Tennessee Mouse Genome Consortium supported by a cooperative of NIH institutes is performing a large scale regional ENU screen for mice displaying recessive abnormalities in behavior and the central nervous system Point mutations induced by ENU likely mimic subtle human to human genetic variation including variation that modifies longevity and age related functional decline The design of our screen offers an opportunity to maintain many parallel cohorts of animals homozygous for the same mutagenized chromosome allowing for a screen for heritable differences in how individuals age For each pedigree animals are screened at seven weeks after birth In addition eight naïve animals from each pedigree are set aside for rescreening at 18 months Age onset phenotypes of interest include early morbidity mortality and progressive neuromuscular or neurological abnormalities The first of 150 pedigrees will reach 18 months of age by Fall 2002 with a steady state of additional pedigrees analyzed thereafter Germ cells from each pedigree are cryopreserved early so that late onset mutations can be recovered if fertility is compromised by age We are likewise initiating a pilot study to evaluate aging pedigrees for increased longevity by maintaining 100 pedigrees for an additional

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file99.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    J 1 Oxspring R 1 Selley R 1 Seymour M 2 Johnson R 1 Informatics Group MRC Harwell 2 Mary Lyon Centre MRC Harwell The Mary Lyon Centre MLC is a state of the art central facility for mouse genetic studies being constructed on the MRC Harwell UK campus An important aspect of the LIMS system for the MLC is the ability to allow technicians to record and logically store data relating to the genotype and phenotype of animals in their care Previous electronic data storage systems implemented at MRC Harwell have used free text to allow users to enter this data This approach can make statistical analysis of the data difficult as there is a lack of standardisation of the data set The AnonyMus system will employ a relational database structure to allow the storage of a library of mouse genes alleles and phenotypes It will be possible to associate these records with the mice in the system as genotypes and phenotypes of interest The users will be prompted to record their phenotypic observations throughout the animal s life Suitably qualified individuals will be able to record the genotypes of these animals based upon phenotypic observations the animal s

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file100.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    OF Odz4 A CAUSE OF PLEIOTROPIC EMBRYONIC LETHALITY A Lossie Baylor College of Medicine Justice MJ Baylor College of Medicine One of the most important outcomes of the large ENU mutagenesis screens is the creation of an allelic series where multiple mutations occur in a single gene When the lesions are identified the biological functions of the different protein domains become apparent One of the most mutated loci in the albino region is l7Rn3 with 6 independent mutational events each of which has a distinct phenotype The m6 allele has two different phenotypes it is embryonic lethal when maternally inherited as a hemizygote but viable when paternally inherited or homozygous The m6 lethals have primary mesoderm extraembryonic and neural tube defects Candidate gene and mutation analyses have identified Odz4 as the best fit for the multiple phenotypes of l7Rn3 Odz4 is a complex locus spanning 650 kb of genomic DNA and encoding several alternatively spliced transcripts that range in size from 1 12 kb Two Odz4 cDNAs totaling 9815 bp have been isolated from mouse fibroblast and adult brain libraries Northern analyses show ubiquitous expression of a 4 5 kb transcript while the 2 and 12 kb mRNAs demonstrate tissue

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file102.shtml (2016-02-17)
    Open archived version from archive



  •