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  • The 16th International Mouse Genome Conference (2002)
    SELECTIVE ALLOGENEIC STERILITY MUTANTS BY ENU MUTAGENESIS X Du The Scripps Research Institute Whitten C Mann N Beutler B The Scripps Research Institute Maternal acceptance of the trophoblast a semi allograft by virtue of its content of paternal genes is one of the central mysteries of immunology While most tissue allografts e g transplanted organs are rejected by the adaptive immune system the placenta provokes essentially no immune response Disruption of the system for maternal tolerance should produce a state of selective allogeneic sterility and ENU is being used to create female mice with germline mutations that produce this phenotype F1 and F3 C57BL 6J females with germline mutations are enrolled in a screen to detect mutations that disrupt maternal tolerance In detail female mice are first outcrossed with C3H HeN males Within 90 days of exposure to an allogeneic male 99 of normal mice have undergone parturition Any mutant mouse that has been exposed to a C3H HeN male for more than 90 days without achieving parturition is enrolled in a syngeneic cross Syngeneically sterile females are discarded while syngeneically fertile females are again crossed to C3H HeN males to confirm allogeneic sterility Female mice that show selective allogeneic

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file120.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    S Simon M Mallon AM Nolan P Green E Hancock JM Medical Research Council Mammalian Genetics Unit The MRC at Harwell is engaged in an extensive mutagenesis programme that aims to generate mouse stocks with a high coverage of the mouse genome for post genomic analysis For this resource to be made accessible to the wider community we have developed software solutions to maximise the researcher s ability to find strains of interest The latest of these implemented within the Mutabase system developed at Harwell allows phenotypic data to be captured at the point of generation and incorporates a powerful search engine The search engine uses web based technologies to make available a high level phenotype ontology developed in collaboration with the Jackson Laboratory combined with a specific keyword search to facilitate the identification of a mouse of interest The software also includes an ordering system to assist in the smooth transition from identification to appropriation of the mouse line To allow researchers to be notified immediately when the programme has generated mice with a phenotype of interest a module underlying the mouse husbandry system automatically triggers an email to be sent when a new mouse is added We also

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file121.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    K Peters L Svenson K O Brien T The Jackson Laboratory Gene based approaches for exploring function and phenotype in the laboratory mouse are powerful and efficient but are not sufficient due to the complexity and redundancy of biological processes in mammalian systems Phenotype driven approaches to understanding biological complexity are complementary to gene driven approaches but suffer from a reliance on the availability of appropriate mutations leaving a significant gap between the universe of possible phenotypes and those that are available for study The Jackson Laboratory TJL has established two large scale mutagenesis centers to generate new mouse models for human disease and to close the phenotype gap The TJL Neuroscience Mutagenesis Facility seeks to produce new mouse models of human neurological disease The TJL Mouse Heart Lung Blood and Sleep Disorders Center links quantitative trait loci QTL and single gene mutations to gene function and disease and seeks to dissect the genetic variation underlying complex cardiovascular lung hematopoietic and sleep dysfunction Both mutagenesis centers at The Jackson Laboratory generate new mutations using ethyl nitro sourea ENU in both genome wide and specific genomic region screens using normal C57BL 6J mice and sensitized mutant mice This classic strategy is

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file122.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    1 J Y Le Gall 1 CNRS UMR6061 2 INSERM CreS Centre de Recherche d Immunologie et d Hématologie Strasbourg 3 INSERM U567 CNRS UMR8104 laboratoire membre de l IFR116 4 Faculté de Médecine de Rennes 5 Washington University School of Medicine St Louis Hereditary hemochromatosis HH a common autosomal recessive disorder due to a mutation in HFE1 is characterized by excessive absorption of dietary iron Little is known however of the apparently complex pathophysiology of HFE involvement in the process of non heme iron uptake and transport to the blood Here in order to tackle the issue in vivo we decided to target HFE expression exclusively to the relevant tissue intestinal epithelium This was achieved by putting HFE under transcriptional control of the rat fatty acid binding protein Fabpi promoter Quite unexpectedly two out of three Fabpi HFE transgenic lines had significantly elevated serum transferrin saturation levels in comparison to those of normal littermates By a careful layer by layer analysis of transgene expression along the crypt villus axis we showed that the ectopic expression of transgenic HFE in the differentiated villi enterocytes was responsible for ferric hyper absorption a phenomenon exacerbated in the absence of endogenous HFE expression

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file123.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    Photographs Awards POSTER 125 SiRNA DESIGN EXECUTION AND ANALYSIS A Goodwin Ambion Inc Brown D Ford L Jarvis R Byrom M Pallotta V Khoeunh T Ambion Inc Recent reports indicate that direct transfer of 21 23 base small interfering RNAs siRNAs into mammalian cells can effectively target specific RNAs for gene silencing Here siRNA efficacy mechanism of action distribution induction and duration of action were addressed In vitro transcribed siRNAs to mammalian genes including GAPDH beta actin cyclophilin c myc c jun c fos survivin and the RNA binding protein La had a 20X increased potency over chemically synthesized siRNAs for suppression of target expression siRNAs were localized to the nuclear periphery and in vivo siRNA strand separation was observed Both mRNA and protein levels remained suppressed as long as 10 days post transfection The Silencer TM siRNA Construction Kit provides a cost effective alternative to chemically synthesized siRNAs yielding molecules of higher potency Transfection agents optimized to deliver siRNA to a wide variety of mammalian cells at high efficiency siPORT Amine a polyamine mixture and siPORT Lipid amixture of cationic and neutral lipids will also be described Ambion s Silencer TM siRNA Labeling Kit can append either Cy3 or

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file125.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    TO ISOLATE CARDIOVASCULAR AND HEMATOPOIETIC LETHAL MUTATIONS USING A MOUSE BALANCER CHROMOSOME K Hentges Baylor College of Medicine 2 Furuta Y 1 Kaiser C 2 Wang Y 1 Lai L 1 Hirschi KK 1 Schwartz R 2 Johnson RL 3 Bradley A 1 Justice MJ 1 Baylor College of Medicine 2 University of Texas MD Anderson Cancer Center 3 Sanger Centre Balancer chromosomes are a useful tool for mutation isolation however they have not previously been used in mice We used a Cre loxP engineered Chromosome Chr 11 balancer in a genetic screen designed to isolate recessive mouse mutations induced by N ethyl N nitrosourea ENU We have isolated 40 lines of mice on Chr 11 that are lethal before weaning The time of death has been determined for several mutant lines and ranges from pre implantation to two weeks after birth An examination of mutant phenotypes reveals that many of the mutants are defective in the hematopoetic or cardiovascular system The phenotypes in these mutants include heart formation outside the amnion internal hemorrhages late in gestation and abnormal blood cell counts Markers of cardiac mesoderm vascular endothelium and blood cell development are being analyzed in mutants with phenotypes that

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file126.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    THE LVIS1 LOCUS IN GENE REGULATION AND LEUKEMIA K Weiser Baylor College of Medicine Justice MJ Baylor College of Medicine In an effort to identify genes involved in leukemogenesis AKXD mouse strains with Murine Leukemia Virus MLV in their germline were screened for leukemia and lymphoma The location of somatic insertions was determined by PCR in leukemic animals This screen identified sites of frequent integration leading to leukemia in mice The most frequent insertion site in these mice is lymphoid viral insertion site 1 Lvis1 which was rearranged in 22 of B cell cancers in the initial study Although no genes were located in the Lvis1 region two genes 50 70kb upstream Hex and Eg5 are upregulated on viral insertion in Lvis1 Conversely genes equivalent distances downstream of Lvis1 are not misexpressed Comparing human and mouse sequence at Lvis1 reveals regions of high identity in Lvis1 DNaseI hypersensitivity studies reveal possible sites of hypersensitivity in cell lines which normally express Hex This indicates that Lvis1 may be a site of trans factor binding correlated with expression of Hex We believe that Lvis1 is a genetic control region for Hex and possibly Eg5 and that these genes are part of a

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file127.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    of Medicine Justice MJ Baylor College of Medicine AKXD recombinant inbred RI mice are susceptible to developing a variety of leukemias and lymphomas which are associated with somatically acquired proviruses These proviruses integrate throughout the genome activating cellular proto oncogenes and or disrupting tumor suppressor genes thereby leading to tumorigenesis Multiple common sites of integration have been identified in mice but recently a novel site of integration lymphoid viral insertion site Lvis1 was identified and shown to be the most common site of integration found in tumor samples taken from AKXD RI mice Analysis of gene expression in the region of Lvis1 showed two genes Hex and Eg5 whose expression levels were altered in tumors containing retroviral integrations at Lvis1 Hex is a divergent homeobox gene expressed during embryogenesis and in B cell and myeloid cell lineages in the adult mouse Eg5 is a member of the bim c kinesin like motor protein family and is believed to be involved in mitotic spindle formation and stabilization Our goal is to test the hypothesis that Hex and or Eg5 can act as an oncogene when overexpressed The approach I have taken is to generate transgenic mice which overexpress Eg5 in hematopoietic

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file128.shtml (2016-02-17)
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