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  • 18th International Mouse Genome Conference (2004)
    and Neurobiology University of Tennessee Health Sciences Center Memphis United States With the goal of discovering genes that extend the healthy life span in mammals we are phenotyping mice carrying new mutations induced by N ethyl N nitrosourea ENU The phenotyping plan includes evaluation for markers of growth trajectory and stress response in pedigrees aged to 28 months and beyond For the aging core four males and four females of the test class are group housed at weaning to await screening at 18 months for behavioral and neuroanatomical abnormalities and at 19 24 months for factors thought to be predictive of longevity Mice of the genotype that is homozygous for a mutagenized G 0 grandparental target chromosome constitute the test class that should manifest any obvious or subtle abnormal recessive phenotypes caused by a new ENU induced mutation This test class can be identified by visual or molecular markers To date we have aged 202 pedigrees beyond 18 months and screened for body weight blood glucose leptin insulin insulin like growth factor 1 corticosterone and ability to maintain body temperature in the cold We have identified phenodeviants for early low and high body weight high corticosterone high and low blood

    Original URL path: http://www.imgs.org/Archive/abstracts/2004abstracts/abs/file173.shtml (2016-02-17)
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  • 18th International Mouse Genome Conference (2004)
    the NCI to implement a collaborative project of mouse cancer modeling The resulting research program the Mouse Models of Human Cancers Consortium MMHCC has expertise in many aspects of basic translational clinical and epidemiological research and mouse genetics The MMHCC is continually challenged by the NCI to identify the most pressing questions in cancer biology and translational science and to direct cancer modeling skills to those questions The initial program of 19 research groups was recently expanded to 25 the increased size requires the NCI to revisit how to manage the MMHCC to preserve the free exchange of ideas and spirit of cooperation that are hallmarks and great strengths of the program The 250 member MMHCC cooperates with the NCI to evolve an integrative systems approach to human cancer research The NCI Center for Bioinformatics integrates descriptive cancer model information with comparable human disease data The Center maintains the Cancer Models Database and the Cancer Images Database to house descriptive data about all types of in vitro and in vivo cancer models Any researcher may submit data to these databases to ensure that the information store reflects the experience of the community of cancer researchers who explore how well the

    Original URL path: http://www.imgs.org/Archive/abstracts/2004abstracts/abs/file174.shtml (2016-02-17)
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  • 18th International Mouse Genome Conference (2004)
    Center Albany NY United States Genetic and environmental interactions are common when studying complex traits In the literature there have been several suggestions that diet especially a high fat diet may play a role in mouse behaviour Therefore the effects of a high fat diet on behaviour was investigated in two inbred strains of mice C57BL 6J B6 and 129S1 SvImJ 129S1 Fifty mice of each strain were placed on an atherogenic diet Paigen s Diet for 14 weeks and compared in parallel to 50 mice on a standard laboratory chow diet As expected this diet significantly affected average plasma cholesterol values 136 vs 242 mg dl in 129S1 and 105 vs 276 mg dl in B6 The diet also affected several other blood parameters including levels of alanine aminotransferase alkaline Phosphatase glucose amylase and calcium Sometimes these changes were observed in only in one of the two strains For example calcium levels were higher in the 129S1 strain on a high fat diet yet the same was not true for B6 Two measurements of behaviour were also affected by diet but only in B6 mice On the high fat diet B6 mice showed lower open field activity and a

    Original URL path: http://www.imgs.org/Archive/abstracts/2004abstracts/abs/file175.shtml (2016-02-17)
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  • 18th International Mouse Genome Conference (2004)
    INSERTIONAL MUTAGENESIS PROJECT FOR THE MOUSE Poirier C Overbeek PA Adams CP Harrison WR Xiao N Castile CA Bishop CE Baylor College of Medicine Houston United States We developed a 2 step insertional mutagenesis program for the mouse using the FVB N albino inbred strain All transgenes carried a visible semidominant coat color marker tyrosinase flanked by the inverted terminal repeats specific for the Sleeping Beauty transposase In the first step we generated regular transgenic mice with our tyrosinase transgenes and transmission of the transgene was followed with the visible coat color marker In the second step the transgene was transposed from its original insertion site through the cut and paste system mediated by the Sleeping Beauty transposase For transpositional purposes we generated different transgenic lines in the FVB N background which expressed the transposase from 4 different promotors Transposition in the paternal germline was identified by new variations of pigmentation in the progeny In both steps heterozygous and homozygous mice were easily identified by their coat color and were screened for inherited abnormal phenotypes From this phenotype driven mutagenesis program the causative genes were subsequently cloned using the transgene transposon as a probe Several tyrosinase transgene constructs have been

    Original URL path: http://www.imgs.org/Archive/abstracts/2004abstracts/abs/file176.shtml (2016-02-17)
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  • 18th International Mouse Genome Conference (2004)
    EMBRYONIC STEM CELLS Greber B Lehrach H Himmelbauer H Max Planck Institute for Molecular Genetics Berlin Germany Despite the availability of mammalian genome sequences the functions of many genes remain unknown Mutant mice are valuable for studying mammalian gene functions and for modeling human disease phenotypes With regard to the latter a range of diseases involves the missplicing of pre mRNAs Embryonic stem cell technology offers the possibility to manipulate the mouse genome and select for mutations in vitro Mutant animals can then be generated from selected clones We are inducing mutations chemically using ENU followed by the generation of a mutant clone library in a 96 well format The library we have generated consists of about 40 000 clones distributed into 96 times 96 samples Such clone libraries can be specifically screened for mutations in genes of interest We have developed a protocol that allows the targeted detection of splice mutations based on highly pooled cDNA samples The procedure does not require any high cost instrumentation and is time efficient enabling one worker to screen a given gene within about one week As a proof of principle we have isolated splice mutant clones for the mouse Kit gene and

    Original URL path: http://www.imgs.org/Archive/abstracts/2004abstracts/abs/file177.shtml (2016-02-17)
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  • 18th International Mouse Genome Conference (2004)
    H Mandillo S Marazziti D Meziane H Nolan PM Ouagazzal A Parker A Pedersen V Rosenthal N Tocchini Valentini G Wurst W 1 MRC Harwell United Kingdom 2 ICS Strasbourg France 3 EMBL Monterotondo Italy 4 CNR Monterotondo Italy 5 GSF Munich Germany 6 EMBL Monterotondo Italy EUMORPHIA is a European Consortium focused on the development of new approaches in phenotyping mutagenesis and informatics leading to improved characterisation of mouse models for the understanding of human physiology and disease European mouse geneticists with different and varied expertise are working together in order to provide the scientific community with a platform for the systematic and standardised phenotyping of mouse models Eumorphia members from different centres are grouped into workpackages WP each dealing with a set of specific body systems Workpackage 10 responsible for behaviour and cognition phenotyping identified a high throughput battery of 9 primary tests to be included in the first line screen These tests are open field modified SHIRPA grip strength assessment rotarod assessment y maze spontaneous alternation pre pulse inhibition of acoustic startle response tail flick test tail suspension test and swim ability assessment Here we will present and compare data obtained on four inbred strains C57Bl 6J

    Original URL path: http://www.imgs.org/Archive/abstracts/2004abstracts/abs/file178.shtml (2016-02-17)
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  • 18th International Mouse Genome Conference (2004)
    Cook M 2 Jablonski M 1 Smeyne R 1 Elberger A 1 Johnson DK 4 1 UT Health Science Center Memphis TN United States 2 University of Memphis Memphis TN United States 3 St Jude Children s Research Hospital Memphis TN United States 4 Oak Ridge National Laboratory Oak Ridge TN United States The Tennessee Mouse Genome Consortium TMGC consists of researchers from eight research institutions Members from five of these institutions are part of one of the three NIH funded groups to perform ENU mutagenesis and screen for neurological phenotypes The mutagenesis protocol is designed to detect recessive mutants whose mutated gene is localized to a specific region of the genome This regional mutagenesis using visible or molecular markers permits the a priori identification of test class mice that can be tested as isogenetic cohorts This helps greatly with the aging of pedigrees to 18 and 28 months identification of pedigrees that have developmental lethal mutations and providing more power for statistically sensitive traits To date we have identified over 80 mutant lines of which 26 of these have neurological phenotypes that are identified in the screening protocols and 9 are visible balance or ataxic mutants The 26 neurological

    Original URL path: http://www.imgs.org/Archive/abstracts/2004abstracts/abs/file179.shtml (2016-02-17)
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  • 18th International Mouse Genome Conference (2004)
    1 Suzuki T 1 Wada Y 1 Wakana S 1 Minowa O 1 Gondo Y 3 Shiroishi T 1 Noda T 1 1 Mouse Functional Genomics Research Group RIKEN GSC Tsukuba Japan 2 Radiation Biology and Medicine Hiroshima University Hiroshima Japan 3 Population and Quantitative Genomics Team RIKEN GSC Yokohama Japan To identify mouse models of human diseases we are running a blood test screening in a large scale ENU mutagenesis program in RIKEN GSC since 2000 The blood test screening system consists of hematological test and clinical biochemical test For each test blood samples are collected at early onset stage 9 weeks for hematology and 11 weeks for clinical biochemistry and late onset stage 52 weeks for hematology and 54 weeks for clinical biochemistry For dominant screening we have screened so far about 10000 mice for early onset stage and about 2000 for late onset stage in each test In early hematological test about 70 of phenodeviants were tested for heritability and half of phenodeviants were inherited In the case of early clinical biochemical test about 200 phenodeviants were preceded to the inheritance test and one forth of them showed heritability In late onset screening about 100 phenodeviants including

    Original URL path: http://www.imgs.org/Archive/abstracts/2004abstracts/abs/file180.shtml (2016-02-17)
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