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  • 17th International Mouse Genome Conference (2003)
    M T The Genomics Institute of the Novartis Research Foundation Co Authors Batalov S McClurg P Barnes S W Wiltshire T Institutions The Genomics Institute of the Novartis Research Foundation Haplotype analysis or determination of ancestral inheritance in chromosome evolution has the potential to increase the speed and efficiency in which phenotypic traits are mapped in both humans and model organisms A high resolution haplotype map will allow for genome wide in silico QTL analysis in mice To this end we have previously assembled a haplotype map of 6 common inbred strains of mice based on sample sequencing of loci relatively evenly spaced across the genome This type of map produced haplotype blocks with a resolution of approximately 2 Megabases To increase the scope and precision of this map we are currently genotyping the 48 designated mouse strains of the JAX Phenome Project with 10 000 SNP based markers Currently we have 5000 markers typed for these DNAs giving a dense set of markers for any strain pair mapping combinations Despite the limitations of the biallelic nature of the SNP markers this preliminary data has provided insights into the breeding lineage of these mice as well as indicating that the

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file12.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    3 van Sloun P 3 Schnütgen F 4 Füchtbauer EM 5 Franz Vauti 5 Arnold HH 1 Hansen J 3 von Melchner H 1 Wurst W Institutions 1 GSF Institute of Developmental Genetics 2 Max Planck Institute Berlin 3 University of Frankfurt 4 University of Arhuis 5 Technical University of Braunschweig Gene trap mutagenesis in mouse embryonic stem ES cells is a complementary approach to the functional annotation of the mouse genome In concert with chemical ENU mutagenesis this approach is accelerating the analysis of gene function in the context of the entireorganism and thus furthers our understanding of human disease We have established a Research Consortium German Gene trap Consortium GGTC to carry out large scale gene trap mutagenesis in ES cells Its goal is to contribute to the saturation mutagenesis of the mouse genome and to generate a mouse model for each gene in cooperation with the International Mouse Mutant Consortium IMMC Towards this goal the GGTC has generated 20 000 mutant ES cell lines by utilizing four different vectors and it has identified the gene trap integration sites in 11 626 clones Of the generated gene trap sequence tags GTSTs 7 453 informative sequences were obtained of

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file13.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Sponsor Exhibitor List Awards Photographs ORAL PRESENTATION MONDAY 10 NOVEMBER 10 15 10 30 HRS FUNCTIONAL ANNOTATION OF MUTANT MOUSE ES CELL LINES BY EXPRESSION PROFILING Symula D J Wadsworth Center Co Authors Curley B Institutions Wadsworth Center A high throughput in vitro phenotype screen would allow powerful forward genetic approaches to be applied to mouse embryonic stem ES cell mutagenesis experiments We have assessed the ability of expression profiling to detect the effects of heterozygous mutations in ES cells and to specifically and accurately categorize mutants Heterozygous mutation in either of two well characterized genes the cell cycle regulator cyclin D kinase 4 Cdk4 or cholesterol transport regulator ATP binding cassette transporter family A member 1 Abca1 resulted in expression profile changes highly specific to each mutant line Few genes associated with pluripotency were differentially expressed between either mutant and its parental cell line Rather the functions of many of the affected genes were consistent with the reported functions of the mutant gene in each ES cell line The heterozygous Cdk4 mutation affected expression of many genes involved in cell growth and proliferation while the heterozygous Abca1 mutation altered genes associated with lipid homeostasis the cytoskeleton and vesicle trafficking

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file14.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    A WHOLE GENOME MOUSE EXPRESSION MICROARRAY USING THE NIA MOUSE GENE INDEX TO EXPAND THE NIA MOUSE DEVELOPMENT AND NIEHS TOXICOGENOMICS MICROARRAY PLATFORMS Carter M G National Institute on Aging Co Authors Sharov A A Dudekula D B VanBuren V Qian Y Ko M S H Institutions National Institute on Aging For global gene expression studies a microarray platform containing all known transcripts expressed in the mouse in all tissues developmental stages and disease states is an important resource which has yet to be fully achieved Previously we developed an in situ synthesized 60 mer oligonucleotide microarray platform Mouse Development Microarray containing 22 000 probes for transcripts isolated by the NIA Mouse cDNA project as cDNA clones primarily from early developmental stages and cultured stem cells Carter et al Genome Res 2003 The NIEHS Toxicogenomics Research Consortium has used the same technology to develop a 21 000 probe microarray platform focused on a curated list of toxicology related transcripts To approach the goal of a mouse whole genome expression microarray we are currently developing an expanded microarray platform based on the NIA Mouse Gene Index a set of almost 30 000 gene transcript models which integrates NIA Mouse cDNA sequences

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file15.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    11 30 11 45 HRS GENE EXPRESSION PATTERNS OF THE MOUSE TRANSCRIPTOME Wiltshire T Genomics Institute of the Novartis Research Foundation Co Authors Walker J Su A Batalov S Hogenesch J Institutions Genomics Institute of the Novartis Research Foundation We have recently completed an analysis of the gene expression patterns of the complete mammalian transcriptome for mouse and human We used high density oligonucleotide arrays to interrogate the mRNA expression of 35 000 human and mouse genes in a diverse set of tissues cell lines organs and pathological samples Human tissue samples and cell lines were obtained from commercial sources and ongoing research collaborations Mouse tissue samples were derived from dissections A total of 80 human and 60 mouse tissues were analyzed for gene expression across all transcripts These data have been curated and compiled with annotation gathered from the public domain and integrated with genomic sequence data They will be publicly available in our searchable GeneAtlas database We have linked these data to an additional search tool GexView Gene Expression View which provides the recall of the expression patterns of all transcripts within a specified genomic region These data have already been used to facilitate the identification of candidate

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file16.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Machka C Institute of Experimental Genetics GSF Research Center for Environment and Health Co Authors 2 Halder T 2 Harder A 1 Hrabe de Angelis M 1 Beckers J Institutions 1 Institute of Experimental Genetics GSF Research Center for Environment and Health 2 TopLab GmbH Martinsried The Delta Notch signal transduction pathway is conserved between many species During the embryonic development of the mouse the Delta Notch pathway is involved in diverse patterning processes lateral specification during neurogenesis and pancreatic development left right organization and involvement in processes like the synchronization organization of the segmentation clock and establishment and maintenance of somite boundaries during somitogenesis are some of them Only a few of these developmental processes can be explained adequately by the classical Delta Notch pathway model describing lateral inhibition In order to identify novel targets of Delta1 on RNA as well as protein level we started to screen transcriptome and proteome using DNA chip technology and 2D gelelectrophoresis combined with mass spectrometry of Delta1 Dll1 deficient and Dll1 wildtype embryos at E10 5 Until now we have identified 26 upregulated and 20 downregulated genes using a DNA chip containing more than 20 000 genes 22 of the identified candidate

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file17.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    of Contents Sponsor Exhibitor List Awards Photographs ORAL PRESENTATION MONDAY 10 NOVEMBER 12 00 12 15 HRS HIPPOCAMPAL GENE EXPRESSION IN HETEROGENEOUS STOCK HS MICE SELECTED FOR LOW MID AND HIGH PERFORMANCE ON COGNITIVE TASKS Paya Cano JL Social Genetic and Developmental Psychiatry Research Centre Institute of Psychiatry King s College London Co Authors Fernandes C Liu L Rijsdijk F Plomin R Schalkwyk LC Institutions Social Genetic and Developmental Psychiatry Research Centre Institute of Psychiatry King s College London Heterogeneous Stock HS mice exhibit marked differences in behavioural phenotypes and it is possible to select individuals on a trait of interest according to their phenotypic variation within a population In this study we examined hippocampal gene expression profiles associated with different levels of performance on cognitive tasks A total of 270 HS mice were tested on a behavioural battery of tests including the light dark box open field plus maze open field novel object Morris water maze puzzle box and tail suspension A phenotypic composite index from the cognitive set of tests was generated for all mice From the resulting individual scores we selected three groups of 27 mice representing the top middle and bottom 10 of the distribution Hippocampal

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file18.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Mouse System Biology Bioinformatics Multigenic and Multifactorial Trait Analysis Nutrition and Metabolic Disease Phenotyping Methods Imaging The Genetics and Genomics of Infectious Disease Verne Chapman Memorial Lecture Table of Contents Sponsor Exhibitor List Awards Photographs ORAL PRESENTATION MONDAY 10 NOVEMBER 14 15 14 30 HRS ANXIETY SUSCEPTIBILITY QTL IN MOUSE MODEL HOW FAR ARE WE FROM DISCOVERING A QT GENE Yalcin B WTCHG University of Oxford Co Authors Fullerton J Miller S Mott R Flint J Institutions WTCHG University of Oxford Using HS mice we have fine mapped an anxiety susceptibility QTL on mouse chromosome 1 to an interval of 0 8 cM We have established a 4 8 Mb high resolution integrated BAC based map underlying this region encompassing 10 genes 8 of which are known B3Galt2 Glrx2 Ssa2 Uchl5 including 4 members of the RGS gene family and 2 unknown genes CDC73 homologue and retinoic acid inducible neural specific protein homologue We sequenced all the genes in each of the 8 HS founders strains We did not find any mutations that alter the genes We have used a statistical approach to assign probabilities to all non coding polymorphisms identified as possible Quantitative Trait Nucleotides Using a combination of

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file19.shtml (2016-02-17)
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