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  • 17th International Mouse Genome Conference (2003)
    Models of Human Disease Mouse System Biology Bioinformatics Multigenic and Multifactorial Trait Analysis Nutrition and Metabolic Disease Phenotyping Methods Imaging The Genetics and Genomics of Infectious Disease Verne Chapman Memorial Lecture Table of Contents Sponsor Exhibitor List Awards Photographs ORAL PRESENTATION TUESDAY 11 NOVEMBER 15 15 15 30 HRS EUMORPHIA DEVELOPING NEW APPROACHES TO MOUSE PHENOTYPING Brown SDM The Eumorphia Consortium EUMORPHIA is an integrated research programme supported by the EU involving the development of new approaches in phenotyping mutagenesis and informatics leading to improved characterisation of mouse models for the understanding of human physiology and disease The project harnesses the expertise and resource of many European mouse genetics centres The focus is on the development standardisation and dissemination of primary and secondary phenotyping protocols for all body systems in the mouse One of the key aims is to improve the reproducibility of phenotype tests and their comparability across different laboratories and to provide access to new and improved phenotyping platforms An initial goal of EUMORPHIA is to establish a new robust primary screening protocol the European Comprehensive First Line Phenotyping protocol ECFLP and to integrate this platform with appropriate ontologies for data dissemination and analysis We will discuss the

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file36.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    14 Lengeling A 12 Lewin G 12 Morano I 15 Müller W 9 Reitmeir P 13 Schmidt J 12 Willnow T 1 Adamski J 1 Beckers J 7 Behrendt H 6 Busch D 16 Favor J 11 Gekeler F 5 Graw J 4 Heldmaier G 2 Heyder J 3 Höfler H 4 Klingenspor M 11 Klopstock T 16 Meitinger T 7 Ollert M 3 Quintanilla Martinez L 8 Wolf E 5 Wurst W 10 Zimmer A Institutions 1 Institute of Experimental Genetics GSF Research Center for Environment and Health Neuherberg 2 Institute of Inhalation Biology GSF Research Center of Environment and Health Neuherberg 3 Institute of Pathology GSF Research Center of Environment and Health Neuherberg 4 Department of Biology Animal Physiology Philipps Universität Marburg 5 Institute of Developmental Genetics GSF Research Center of Environment and Health Neuherberg 6 Institute of Medical Microbiology Immunology and Hygiene Technical University Munich 7 Division of Enviromental Dermatology and Allergy Technical University of Munich 8 Lehrstuhl für Molekulare Tierzucht und Biotechnologie Ludwig Maximilians University Munich 9 IGM GSF Research Center of Environment and Health Neuherberg 10 Universitätsklinikum Bonn 11 Department of Neurology Ludwig Maximilians University Munich 12 Max Delbrück Centrum für Molekulare Medizin Berlin 13 Department of Comparative Medicine GSF Research Center of Environment and Health Neuherberg 14 Research Group Infection Genetics German Research Centre for Biotechnology 15 Department of Experimental Immunology German Research Centre for 16 Institute of Human Genetics GSF Research Center of Environment and Health Neuherberg With the growing number of new interesting and valuable mouse mutants generated by various strategies e g knock out gene trapping or ENU mutagenesis the need for a comprehensive and standardized phenotype characterization of these mouse models rises A comprehensive analysis via a set of parameters ranging over all fields of disease areas will enable us

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file37.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    METHODS FOR EVALUATING HEART LUNG AND SLEEP FUNCTION IN MICE Svenson K L The Jackson Laboratory Co Authors 2 Galante R Pack A I 3 Walker J K Schwartz D A 1 Barker J E Peters L L Institutions 1 The Jackson Laboratory 2 University of Pennsylvania 3 Duke University Given the recent availability of annotated mouse and human genome sequences there is renewed interest in obtaining comprehensive phenotypic information from animal models Classical physiologic analyses as well as newly developed strategies for measuring phenotypic traits in rodents are being implemented in a broad spectrum of research areas in an effort to describe both normal and aberrant health status Using these data it is plausible to link phenotype to gene function in an effort to better understand the genetics underlying human disease The Center for New Mouse Models of Heart Lung Blood and Sleep Disorders at The Jackson Laboratory is conducting high throughput non invasive phenotyping of 1 40 inbred mouse strains and 2 ENU generated mutant mice through the NHLBI funded Programs for Genomic Applications As part of this effort we are developing novel approaches for conducting high throughput primary non invasive assessment of phenotypes relevant to heart lung

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file38.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    C Baylor College of Medicine Anatomic and clinical pathology evaluations can be extremely valuable in the characterization of mutant mice and in the development of valid mutant mouse models of morbid or unusual conditions or diseases Determination of mutant phenotypes can be complicated by strain associated conditions by infectious agents and by non infectious environmental factors Infectious agents and environmental factors may manifest differently in different mouse strains and under

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file39.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    09 30 09 45 HRS WOUND REPAIR AN IMPORTANT PATHWAY IN RESISTANCE TO L MAJOR INFECTION Foote S The Walter and Eliza Hall Institute Co Authors Handman E Ellso C Speed T Smyth G Sakthianandeswaren A Curtis J Kumar B Institutions The Walter and Eliza Hall Institute We have mapped 3 QTLsin he mouse which modulate response to infection by L major These loci have been isolated onto isogenic backgrounds both susceptible and resistant These animals have a phenotype which is partway between that of the resistant and susceptible parents A compound congenic animal has been bred with all three loci on both susceptible and resistant backgrounds These animals can be fully resistant or susceptible although the penetrance is not as complete as the parental lines Dogma has it that the T cell helper responses are important in determining outcome in this disease We have measured the T cell responses in our congenic animals and found that there is no difference between the congenics and their backcross parental strain indicating that the Th response is not playing any role in these animals We have performed a microarray analysis on infected and unifected macrophages the host cell on compound congenic animals

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file40.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    2 Ebeling C 1 Howard T 2 Martin K 1 McEuen M 1 Paeper B 1 Proll S 1 Snell A 1 Tang P 1 Tittel P 1 Wasnick M 1 Wiegand N 1 Bouck J 1 Ramsdell F 2 Carlson G 1 Schatzman R Institutions 1 Celltech R D Inc 2 McLaughlin Research Institute We are using ENU mutagenesis in mice to identify novel clinically relevant targets in the areas of lymphocyte biology inflammation and autoimmunity The approach involves a three generation recessive screen and we are focusing on phenotypes which mimic desired clinical responses e g suppressed inflammatory response thus improving our chances of directly identifying relevant therapeutic targets We use a complementary set of simple in vitro assays e g activation of T and B cells T dependent and T independent inflammatory responses as well as more complex in vivo screens which are based on classic pharmacologic models of inflammation and immune response e g graft versus host response air pouch and colitis Screening has been performed both on inbred and mixed genetic backgrounds We have developed an integrated laboratory informatic pipeline which enables rapid identification of a candidate interval and the genes contained within as well

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file41.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    15 HRS HOST DEPENDENT VARIATION IN COLONIZATION BY THE GASTROINTESTINAL FLORA Threadgill D W University of North Carolina Co Authors Alexander A D Hench G Institutions University of North Carolina The mammalian gastrointestinal GI tract provides a complex ecosystem for numerous bacterial species that in turn modulate susceptibility to disease and infection Members of the GI flora have been associated with a wide variety of GI homeostatic processes and pathological conditions like colorectal cancer colitis antibiotic associated diarrhea prevention of opportunistic infections through competitive exclusion stimulating the immune system metabolism of toxic compounds and digestion of dietary substances Since the role played by host genetic variation in influencing colonization dynamics is unknown we have developed qPCR assays for each member of the Altered Schaedler s Flora ASF a standardized bacterial flora that is commonly used to control the host flora during experimental studies The eight members of this flora contain four extremely oxygen sensitive EOS a Bacteroides a Flexistipes and two Lactobacilus species We have re derived several strains of mice to be germ free and used these to produce ASF gnotobiotic mice qPCR assays detected significant differences in ASF colonization between 129 and BALB c mice showing that host

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file42.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    TISSUE REMODELING IN MICE DEFICIENT FOR RELAXIN RECEPTOR Agoulnik A Baylor College of Medicine Co Authors Kamat A Feng S Bogatcheva N Bishop C Institutions Baylor College of Medicine Relaxins are peptide hormones produced primarily in reproductive organs In the mouse there are two relaxin genes Rln1 and Rln3 that encode related peptides with an overlapping pattern of expression Deletion of relaxin 1 in mice causes remodeling of connective tissues in several organs Recently biochemical studies have identified two closely related G protein coupled receptors LGR7 and LGR8 GREAT as putative receptors for relaxins Additionally insulin like factor 3 has been identified as a cognate ligand for Lgr8 but not for Lgr7 We have shown that the Lgr7 receptor is expressed in a variety of murine organs including reproductive organs brain heart aorta etc Using insertional gene targeting approach we targeted the lacZ gene into the mouse Lgr7 locus Lgr7 deficient mice have abnormal mammary development with smaller poorly developed nipples Mutant females are unable to deliver milk to their pups and have significantly prolonged length of parturition compared to littermates Examination of sections of the lung in Lgr7 deficient mice reveals pulmonary fibrosis Examination of collagen content using trichrome

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file43.shtml (2016-02-17)
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