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  • 17th International Mouse Genome Conference (2003)
    NOVEMBER 12 15 12 30 HRS PHYSIOLOGICAL BASIS OF RESISTANCE TO DIETARY OBESITY SEEN IN THE B6 LP MOUSE CONGENIC FOR A REGION ON CHROMOSOME 2 Zuberi A R Pennington Biomedical Research Center Louisiana State University Co Authors MacGowan J Richards B Krishnan B Institutions Pennington Biomedical Research Center Louisiana State University A novel B6 LP mice strain has been generated that contains a small 4 cM region derived from Chromosome 2 of the LP J mouse introgressed onto the C57BL 6J strain Male mice are resistant to dietary induced obesity in response to a diet high in fat and sucrose HFHS relative to the obesity susceptible B6 mouse Interestingly these mice also differ in the circulating fasting insulin concentrations when fed either a low fat chow or HFHS diets suggesting that a difference in insulin sensitivity may underlie the difference in obesity phenotype We describe our efforts to discern the physiological basis for these phenotypes by determining the metabolic rate thermogenesis physical activity and food consumption on chow and HFHS fed mice and measurements of glucose tolerance and insulin sensitivity Subcongenic mice containing recombinant chromosomes within the congenic region have been identified and their phenotyping has further refined the

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file44.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Camper SA University of Michigan USA Co Authors 1 Brinkmeier ML 2 Carninci P 1 Lyons RH 2 Shiraki T 2 Arakawa T 2 Kawai J 2 Hayashizaki Y Institutions 1 University of Michigan USA 2 RIKEN Genomic Sciences Center Japan Prop1 encodes a paired like homeodomain transcription factor necessary for normal pituitary growth shape and cell specification It is expressed only in embryonic pituitary peaking at e12 5 PROP1 mutations are the most common cause of multiple pituitary hormone deficiency in humans but few transcriptional targets are known We employed three gene discovery approaches to identify Prop1 targets and novel factors influencing pituitary development Differential display and subtractive hybridization methods applied to RNA prepared from dissected embryonic pituitary primordia proved to be a rich source of novel genes Several members of the Wnt signaling pathway were represented and two Tcf7l2 and Aes were confirmed to have functional roles in pituitary growth and shape The value of these libraries is limited by the short cDNAs typically produced We prepared three full length cDNA libraries from pituitary primordia of normal e12 5 e14 5 and mutant e14 5 embryos using the cap trapper method Our libraries were among the best of

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file45.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    MOLECULAR PATHWAYS FOR MAMMARY TUMOR METASTASIS Hunter K Laboratory of Population Genetics NCI NIH Co Authors Park Y G Yang H Zhang J Lukes L Lancaster M Buetow K Institutions Laboratory of Population Genetics NCI NIH Our laboratory has demonstrated that the genetic background of tumor significantly influences the ability of the tumor to disseminate Using conventional QTL mapping crosses and RI strains we demonstrated the presence of at least 5 genomic regions that were significantly or suggestively associated with metastatic efficiency Conventional congenic mapping experiments are currently in progress to achieve high resolution mapping of candidate loci In parallel to supplement and accelerate the candidate gene identification process we have been performing an integrative trans disciplinary strategy utilizing a variety of omic technologies to develop a more comprehensive pathway based understanding of the metastatic process Combining genetic somatic cell genomics bioinformatics haplotype mapping microarray analysis proteomics and genome wide transcriptional QTL mapping we have identified a molecular process that plays an important role in metastatic progression The identification of this pathway as a potentially important element in tumor dissemination has recently been supported by the confirmation that the murine ortholog of the breast cancer metastasis suppressor gene Brms1 is

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file46.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    15 HRS MAKING SENSE OF SEQUENCE INTEGRATING BIOLOGICAL KNOWLEDGE WITH THE MOUSE GENOME Bult CJ The Jackson Laboratory Co Authors Bradt D Reed D Zhu S Qi D Sinclair B Blake J A Ringwald M Eppig JT Richardson J E Kadin J A The Mouse Genome Informatics Group Institutions The Jackson Laboratory The release of the publicly accessible mouse genome sequence for the C57BL 6J strain of the laboratory mouse represents a landmark event in genome biology The ability of researchers to use the mouse genome sequence effectively will depend in large part on how well the genes and other features identified in the sequence are integrated with the biological data sets available for the mouse that are available from the Mouse Genome Informatics MGI database Model organism databases such as MGI have a unique role to play in connecting sequence and biology and in curating these connections for the long term The ways in which sequence data are stored and subsequently accessed from MGI are changing rapidly Results of these significant enhancements to the capacity of the database will better enable the mouse genetics and genomics research communities to find biological meaning in the mouse genome sequence I will

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file47.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    INTEGRATED APPROACH TO MOUSE FUNCTIONAL GENOMIC DATA Hedeler C University of Manchester Co Authors Else K J Paton N W Institutions University of Manchester The study of immune response to infection by pathogens provides useful insights for the development of anti parasitic drugs To be of full benefit an understanding of the immune response to infection is necessary this can be gained by applying several analysis techniques Recently the development of new techniques such as microarrays has lead to large scale analysis being used more frequently The outcomes of these techniques are vast amounts of data requiring extensive use of databases and several analysis tools to manage and examine the data efficiently Recent work has addressed this requirement in general but little work has yet been done to address requirements arising out of studies of the immune response To identify the requirements arising out of the study of immune response a systematic characterisation and classification of tasks which are of particular interest to immunologists is presented Fulfilling these tasks requires further information from different data sources e g Gene Ontology Annotation these too are investigated together with their corresponding tasks A prototype implementation that combines tight integration of the identified

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file48.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    and Genetics Siberian Branch of Russian Academy of Sciences Laboratory of Endocrine Genetics Co Authors Osadchuk A V Institutions Institute of Cytology and Genetics Siberian Branch of Russian Academy of Sciences Laboratory of Endocrine Genetics Social dominant subordinate relationships in animal populations are a crucial factor of microevolution However genetic bases of social dominance are poor investigated We studied interstrain differences in the level of social dominance and testis steroidogenic activity in mouse micropopulations consisting of six males one male from each of 6 strains A He CBA Lac C57Bl 6J DD PT and YT Social rank of each male was estimated by winning defeat score in intermale aggressive encounters Basal and stimulated by human chorionic gonadotropin forskolin cholera toxin dibutyryl cAMP and pregnenolone testicular testosterone production in vitro were estimated at informative time points of social hierarchy formation 1 hour 8 and 17 days after grouping Highly significant interstrain differences in the level of social dominance were demonstrated Males of PT strain were characterized by the highest level of social dominance they occupied dominant rank in 44 of 155 micropopulations Dominance level of other strains did not significantly differ from random level It is very interesting that PT strain

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file49.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Cano JL 2 Gregorova S 2 Forejt J 1 Schalkwyk LC Institutions 1 Institute of Psychiatry King s College London 2 Institute of Molecular Genetics Academy of Sciences of the Czech Republic PWD Ph is an inbred mouse strain derived from wild mice trapped in central Czech Republic These mice are of the Mus musculus musculus subspecies whose ancestors separated from those of Mus musculus domesticus about one million years ago There is a high degree of sequence polymorphism and variation in diverse phenotypes between PWD Ph and common inbred mouse strains Gregorova and Forejt 2000 genomes of which are 80 or more Mus musculus domesticus origin making PWD Ph mice a useful resource for complex trait research As a first step in taking advantage of this resource a preliminary characterisation of the behaviour of PWD Ph mice was performed Groups of 10 PWD Ph and C57BL 6J male mice were tested in the open field novel object exploration task and Morris water maze PWD Ph were marginally more anxious than C57BL 6J mice but subsequently displayed higher levels of exploration and lower anxiety than C57BL 6J mice following introduction of a novel object PWD Ph and C57BL 6J mice

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file50.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    HEAT AND CAPSAICIN SENSITIVITY BETWEEN MOUSE STRAINS KJR AND C57BL 6 Furuse T National Institute of Genetics Co Authors Shiroishi T Koide T Institutions National Institute of Genetics Pain sensation is an essential alert for avoiding environmental danger and feeling tissue damage or illness In spite of the essential role of pain sensation surplus pain is stressful or unpleasant for both animals and human Both of heat evoked pain and capsaicin sensation are transmitted to central nervous system via VR1 TRPV1 receptor a multi functional pain receptor Capsaicin is the main chemical component of hot chili peppers providing its hot taste and causes sensation in physiological pain system The difference of pain sensitivity is genetically controlled To elucidate the genetic system of diversity of pain sensitivity we applied both capsaicin sensitivity test and 52 hot plate test for two strains C57BL 6 a laboratory strain and KJR a wild derived strain In these tests KJR indicated lower sensitivity than C57BL 6 for both capsaicin and heat evoked pain We conducted QTL analyses to characterize genetic loci involved in the different sensitivity to capsaicin and heat evoked pain between C57BL 6 and KJR In the study 470 F2 mice were generated

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file51.shtml (2016-02-17)
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