archive-org.com » ORG » I » IMGS.ORG

Total: 854

Choose link from "Titles, links and description words view":

Or switch to "Titles and links view".
  • 17th International Mouse Genome Conference (2003)
    Nolan P MRC Mammalian Genetics Unit Co Authors Bacon Y Breeds S Clay J Ooi A Kerr S Institutions MRC Mammalian Genetics Unit We are conducting a screen in mice for ENU induced mutations affecting circadian rhythms and entrainment to light The screen consists of an assessment of mouse wheel running activity in a 12 12 hr light dark cycle for 7 10 days followed by assessment in constant darkness DD for 20 days Responses to light are assessed using two protocols a a 15 minute light pulse given at circadian time 16 CT16 on the tenth day in constant darkness and b an additional 12 hrs of light upon transition from light dark conditions to constant darkness We have identified one particular dominant mutant line in our screen PLAY 8 where both circadian and light responsive parameters are affected PLAY 8 mutants can exhibit a range of phenotypes including a shortening of the circadian period by one hour free running in light dark conditions arrhythmicity in constant darkness and reduced or absent responses to light using both protocols A genome scan of backcross progeny detected one region of linkage on distal mouse Chr 8 and further fine mapping allowed

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file52.shtml (2016-02-17)
    Open archived version from archive


  • 17th International Mouse Genome Conference (2003)
    List Awards Photographs POSTER 5 IDENTIFICATION OF THE BASIC LEUCINE ZIPPER AND W2 DOMAINS 2 BZW2 AS A PUTATIVE CIRCADIAN QUANTATIVE TRAIT GENE Pletcher M T The Genomics Institute of the Novartis Research Foundation Co Authors Hogenesch J Kay S Wiltshire T Institutions The Genomics Institute of the Novartis Research Foundation Recombinant inbred RI mice have been used for the mapping of genes affecting multiple and widely varying phenotypes without requiring new genotyping data for each mapping exercise This property was exploited in the investigation of quantitative trait loci QTL affecting circadian behavior by applying the ratio of light dark activity rates Toth et al Behavioral Genetics 1999 in 13 CxB RI strains to a 1428 marker high resolution physical map of the strain distribution patterns of these mice A high phenotype genotype correlation was found for a 11 MB region on mouse chromosome 12 Using the gene expression database GeneAtlas http expression gnf org circadian 3 genes were identified within this region as being expressed in the suprachiasmatic nucleus SCN in a cyclical manner consistent with a role in circadian locomotor activity One of these genes Bzw2 contained a strain specific amino acid difference in its coding region Preliminary

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file53.shtml (2016-02-17)
    Open archived version from archive

  • 17th International Mouse Genome Conference (2003)
    MUTANTS WITH NEUROLOGICAL GENES Sezutsu H Population and Quantitative Genomics Team RIKEN Genomic Sciences Center Co Authors 2 Wada Y 1 Takahasi KR 1 Sakuraba Y 1 Kaneko S 1 Fujimoto N 1 Tsuchihashi K 1 Matsumoto R 2 Wakana S 2 Noda T 2 Shiroishi T 1 Gondo Y Institutions 1 Population and Quantitative Genomics Team RIKEN Genomic Sciences Center 2 Mouse Functional Genomics Research Group RIKEN Genomic Sciences Center We have developed a system for gene driven ENU mutagenesis to produce recessive mutants efficiently In our mouse mutagenesis project all the G1 sperms have been cryopreserved in liquid nitrogen to retain any G1 male strains for late onset phenotype screening The genomic DNAs from all the G1 males are also extracted from the testes DNAs were already extracted from more than 6 000 individuals From each G1 s DNA any target genes are amplified by PCR and ENU induced mutations are then identified by direct sequencing or TGCE Temperature Gradient Capillary Electrophoresis The point mutations are then examined in vivo by retrieving the sperm from the liquid nitrogen or natural mating and producing the viable G3 mice To elucidate a molecular basis of behavior emotion and neurodegenerative disease

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file54.shtml (2016-02-17)
    Open archived version from archive

  • 17th International Mouse Genome Conference (2003)
    and Metabolic Disease Phenotyping Methods Imaging The Genetics and Genomics of Infectious Disease Verne Chapman Memorial Lecture Table of Contents Sponsor Exhibitor List Awards Photographs POSTER 7 ENHANCED LEARNING AND MEMORY IN MICE LACKING NA CA 2 EXCHANGER 2 Shin H S Center for Learning and Memory and Division of Life Science Korea Institute of Science and Technology Co Authors 1 Jeon D 1 Yang Y 1 Jeong M 1 Rhim H 2 Philipson K Institutions 1 Center for Learning and Memory and Division of Life Science Korea Institute of Science and Technology 2 Cardiovascular Research Laboratories MRL 3 645 UCLA School of Medicine The plasma membrane Na Ca 2 exchanger NCX plays a role in regulation of intracellular Ca 2 concentration via the forward mode Ca 2 efflux or the reverse mode Ca 2 influx To define the physiological function of the exchanger in vivo we generated mice deficient for NCX2 the major isoform in the brain Mutant hippocampal neurons exhibited a significantly delayed clearance of elevated Ca 2 following depolarization The frequency threshold for LTP and LTD in the hippocampal CA1 region was shifted to a lowered frequency in the mutant mice thereby favoring LTP Behaviorally the mutant

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file55.shtml (2016-02-17)
    Open archived version from archive

  • 17th International Mouse Genome Conference (2003)
    CANDIDATE REGION de la Casa Esperon E Temple University Co Authors 1 Briscoe T 2 Pardo Manuel de Villena F 1 Sapienza C Institutions 1 Temple University 2 University of North Carolina When females of the DDK inbred strain are mated to males of other inbred strains C57BL 6 BALB c the vast majority of the resulting embryos die by the time of implantation The reciprocal crosses non DDK females

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file56.shtml (2016-02-17)
    Open archived version from archive

  • 17th International Mouse Genome Conference (2003)
    Lecture Table of Contents Sponsor Exhibitor List Awards Photographs POSTER 9 VELVET A DOMINANT EGFR MUTATION THAT CAUSES WAVY HAIR AND DEFECTIVE EYELID DEVELOPMENT IN MICE Du X The Scripps Research Institute Co Authors 1 Hoebe K 1 Tabeta K 2 Liu H 1 Mann N 1 Mudd S 1 Crozat K 1 Sovath S 2 Gong X 1 Beutler B Institutions 1 The Scripps Research Institute 2 University of California Berkeley In the course of a large scale program of ENU mutagenesis we isolated a dominant mutation called Velvet The mutation was found to be uniformly lethal to homozygotes which do not survive E13 5 Mice heterozygous for the Velvet mutation are born with eyelids open and demonstrate a wavy coat and curly vibrissae The mutation was mapped to the proximal end of chromosome 11 by genome wide linkage analysis On 249 meioses the locus was confined to a 2 7Mb region which included the epidermal growth factor receptor gene Egfr An A G transition was identified in the Egfr coding region of Velvet mice causing the amino acid substitution D831G This substitution alters an essential triad of amino acids D FG G FG which is normally required for

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file57.shtml (2016-02-17)
    Open archived version from archive

  • 17th International Mouse Genome Conference (2003)
    System Biology Bioinformatics Multigenic and Multifactorial Trait Analysis Nutrition and Metabolic Disease Phenotyping Methods Imaging The Genetics and Genomics of Infectious Disease Verne Chapman Memorial Lecture Table of Contents Sponsor Exhibitor List Awards Photographs POSTER 10 KERATIN 14 CRE MICE PROVE MATERNAL OOCYTIC KERATIN 14 EXPRESSION Hafner M German Research Cente for Biotechnology Co Authors 2 Haase I 3 Scharfetter Kochaneck K 4 Pasparakis M 1 Schippers A 2 Krieg T 1 Müller W Institutions 1 German Research Centre for Biotechnology 2 University of Cologne 3 University of Ulm 4 EMBL Monterotondo While displaying tissue specific Cre mediated deletion in the epidermis tongue and thymic epithelium when inherited from the father three independently generated Keratin 14 Cre K14Cre mouse lines display complete deletion of loxP flanked DNA segments in the offspring if the mother carried the transgene Complete deletion is even observed in littermates that lack the K14Cre allele due to transmission of the transgene to the polar bodies during oogenesis Thus maternally expressed Cre protein remains active within the oocyte until after fertilisation The oocytic K14Cre activity was further proven by reporter gene activation in maturing oocytes in preantral and antral follicles Since Keratin 14 K14 mRNA expression could

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file58.shtml (2016-02-17)
    Open archived version from archive

  • 17th International Mouse Genome Conference (2003)
    M National Institutes of Health NHGRI Co Authors 2 Arnheiter H 1 Pavan W J Institutions 1 National Institutes of Health NHGRI 2 National Institutes of Health NINDS Sox10 is a high mobility group transcription factor critical for the development of glia and melanocytes Mutations in Sox10 have been associated with neurocristopathies in Waardenburg syndrome Type IV patients and in the murine mutant Dominant megacolon While Sox10 is clearly necessary for glial and pigmented melanocyte formation it is unknown whether it is also sufficient to promote their differentiation We have therefore constructed and characterized Sox10 transgenic mice in which a functional Sox10 transgene is regulated by the Dopachrome Tautomerase Dct promoter Embryonic Dct is expressed in Sox10 cells melanoblasts and Sox10 cells retinal pigment epithelium RPE and the dorsal neuroepithelium of the brain Dct Sox10 transgenic mice present with small eyes cataracts circling and hyperkinetic behavior tremors severe imbalance and gradual coat color loss Postnatal eyes show age dependent disorganization of the neural retina NR and localized RPE thickening Most significantly in the homozygotes part of the basal ganglion layer of the NR that is positive for the Sox10 transgene expression produces pigment Since Sox10 directly activates Mitf a transcription

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file59.shtml (2016-02-17)
    Open archived version from archive



  •