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  • 17th International Mouse Genome Conference (2003)
    POINT AND IDENTIFICATION OF PROMOTER USAGE Carninci P 1 Laboratory for Genome Exploration Research Group RIKEN Genomic Sciences Center GSC Yokohama Institute 1 7 22 Suehiro cho Tsurumi ku Yokohama Kanagawa 230 0045 Japan 2 Genome Science Laboratory RIKEN Wako main campus Hirosawa 2 1 Wako Japan Co Authors 1 2 Shiraki T 1 Kondo S 1 Katayama S 1 2 Waki K 1 Kasukawa T 1 Kawaji H 1 2 Nakamura M 1 2 Kodzius R 1 Arakawa T 1 Fukuda S 1 Sasaki D 3 Podhajska A 1 Kawai J and 1 2 Hayashizaki Y Institutions 1 Laboratory for Genome Exploration Research Group RIKEN Genomic Sciences Center GSC Yokohama Institute 1 7 22 Suehiro cho Tsurumi ku Yokohama Kanagawa 230 0045 Japan 2 Genome Science Laboratory RIKEN Wako main campus Hirosawa 2 1 Wako Japan 3 Department of Biotechnology Intercollegiate Faculty of Biotechnology University of Gdansk Medical University of Gdansk ul Kladki 24 80 822 Gdansk POLAND We introduce CAGE Cap Analysis Gene Expression which is based on preparation and sequencing of concatamers of DNA tags deriving from the initial 20 nt from 5 end mRNAs CAGE allows high throughout gene expression and profiling of transcriptional starting site

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file76.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Memorial Lecture Table of Contents Sponsor Exhibitor List Awards Photographs POSTER 29 REGION SPECIFIC ENU MUTAGENESIS ON MOUSE CHROMOSOME 5 Ching Y The Jackson Laboratory Co Authors Wilson L Howell G Hartford S Sears A Schimenti J Institutions The Jackson Laboratory With the completion of the human and mouse genomes functional genomics will become the future of genome research Phenotype driven studies using ENU is one of the systematic tools of genome analysis Our laboratory has been conducting a large scale ENU mutagenesis program designed to isolate ENU induced recessive mutations mapping within or near the Rw inversion The region is associated with a chromosomal inversion spanning 30 cM of the proximal region of mouse chromosome Chr 5 A series of overlapping deletion complexes have been generated across the Rw region can that facilitate systematic functional analyses of the region of interest Based on the principle of allelic noncomplementation ENU induced mutations can be localized to the region of a deletion with a single mating pair G3 mice homozygous for the ENU treated chromosome were screened for morphological anomalies vestibular defects hearing loss sterility and DNA repair defects The screen also incorporated visible markers to allow for identification of recessive

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file77.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Disteche C University of Washington Co Authors Nguyen D K Institutions University of Washington The mammalian sex chromosomes derived from a homologous pair of ancestral chromosomes Divergence between the X and Y chromosomes evolved as recombination between them was suppressed and male advantageous genes accumulated on the Y As a result the Y chromosome lost many genes except for genes involved in testis function and male fertility Concomitantly X inactivation evolved as a mechanism of dosage compensation between the sexes Remnants of this evolution can be found in present day X Y gene pairs Previous studies in human have shown that differentiation of X Y genes occurred in a step wise fashion that defined a series of strata along the X likely due to large Y inversions that inhibited recombination Lahn and Page 1999 These evolutionary strata are highly rearranged in mouse Disteche 1999 We have now examined the divergence of X Y genes in relation to their position on the mouse X We determined that the proportion of silent substitutions Ks between members of X Y gene pairs in mouse was generally higher 1 3 times on average than in human and did not vary in strict correlation to

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file78.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    IN ANIMAL MODELS OF CHRONIC INFLAMMATORY DISEASES AS A TOOL FOR DRUG TARGET DISCOVERY Douni E Biomedical Sciences Research Center Alexander Fleming Co Authors Sekara E Kamber M Mantellou M Kontoyiannis D Kollias G Institutions Biomedical Sciences Research Center Alexander Fleming Random mutagenesis on a genome wide scale is an attractive method to sample without bias the role of virtually all genes in a particular pathological process Mutant mice carrying a deletion of the 3 AU rich elements ARE from the TNF mRNA over express TNF and develop chronic inflammatory poly arthritis and Crohn s like inflammatory bowel disease IBD with 100 incidence at 3 months of age We have been using these TNF Δ ARE mutant mice in a sensitized ENU mutagenesis screen to identify novel genes that modify the disease phenotype We have optimized chemical mutagenesis in the TNF ΔARE mice by careful titration of various ENU doses in their original mixed C57BL 6 x 129SvEv genetic background By using simple and accurate phenotypic screens clinical score for arthritis and macroscopic or histological analysis for IBD we are selecting the individual progeny that no longer suffer from the disease In this approach large numbers of targets can be

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file79.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    1 Institute of Biomaterials Biomedical Engineering University of Toronto Toronto Canada 3 Programme in Development and Fetal Health Samuel Lunenfeld Research Institute Toronto Canada 4 Centre for Modeling Human Disease Toronto Canada Co Authors 1 3 4 Reid T 4 Hant P 1 3 4 Lan Q 1 3 4 Li C 1 3 4 Ohishi M 4 To C 4 Tsao N 1 3 4 Yu M 4 Kassam N 2 4 Osborne L 2 3 4 Rossant J 1 3 4 Stanford W Institutions 1 Institute of Biomaterials Biomedical Engineering University of Toronto Toronto Canada 2 Department of Medical Genetics University of Toronto Toronto Canada 3 Programme in Development and Fetal Health Samuel Lunenfeld Research Institute Toronto Canada 4 Centre for Modeling Human Disease Toronto Canada Gene trap mutagenesis of mouse embryonic stem ES cells generates random loss of function mutations which can be identified by a sequence tag and can often report the endogenous expression of the mutated gene The Centre for Modeling Human Disease CMHD is performing gene trap based expression and genotypic screens to generate new mouse mutations as a resource for the scientific community The gene trap insertions are screened using multiplexed in vitro differentiation and induction assays Data is stored in a MySQL relational database and a user friendly web based interface allows researchers to make advanced queries of the expression data Sequence tags are being generated by 5 RACE 3 RACE and inverse PCR to complement expression profiles Semi automated sequence processing and annotation algorithms are used to identify trapped genes and to extract additional information including chromosomal location gene ontology GO classifications and protein functional domains The sequence tag database can currently be queried using a BLAST interface and by the time of this meeting researchers will also be able to

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file80.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    ANALYSIS OF THE MAMMALIAN GENOME REVEALS NOVEL THERAPEUTIC ENTRY POINTS FOR HUMAN DISEASE Grosse J Ingenium Pharmaceuticals AG Co Authors Stumm G Laufs J Augustin M Sedlmayer R Wattler S Nehls M C Institutions Ingenium Pharmaceuticals AG Key for the successful development of much needed novel therapeutics is the discovery and fundamental understanding of disease processes and the identification of useful intervention points a fact that became even more apparent with the increased failure rate of therapeutic compounds derived from poorly understood genomics based targets In order to speed up the discovery of biomedically important processes Ingenium developed two complementary genetics based technologies Deductive Genomics and INGENOtyping Deductive Genomics allows querying essentially every mammalian gene in its organismal context for the potential function in fundamental disease processes As an example a mouse line with enhanced insulin sensitivity is presented In contrast to insulin sensitizing compounds inducing obesity this animal model presents with decreased body fat content and is resistant to high fat diet induced insulin resistance This provides a new perspective on insulin sensitization as a therapeutic strategy Following the Deductive Genomics based discovery of novel pathways each pathway member is analyzed for its specificity in an animal model before

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file81.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    UPSTREAM REGULATORY SEQUENCES Hafner M Co Authors Institutions Paralogs generated through gene or genome duplication experience identical selective constraints In the long run one of them will thus be turned into an inactive pseudogene To be maintained active paralogs therefore have to gain differences either through the gain of additional functions by dividing the ancestral functions between the daughter genes or through the development of different expression patterns The core promoters of the murine BM 40 SPARC Osteonectin gene and its mammalian orthologs are characterised by the presence of two homopurine stretches and two transcription start points No similarities to promoters of amphibian or invertebrate BM 40 genes or promoters of paralogous members of the mammalian BM 40 gene family could be identified However promoters of mammalian androgen receptor and IGF II genes display a similar architecture and can be aligned to the mammalian BM 40 promoters Band shift and footprinting analyses indicated multiple SP1 binding sites within the homopurine stretches of the human BM 40 gene as has been shown for mammalian IGF II and androgen receptor gene promoters before Additional similarities within the first untranslated exons of BM 40 and IGF II genes and the fact that IGF

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file82.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Contents Sponsor Exhibitor List Awards Photographs POSTER 35 NEUROPILIN 1 A SURFACE MARKER OF REGULATORY T CELLS Hansen W German Research Centre for Biotechnology Co Authors 1 Bruder D 2 Probst Kepper M 1 Westendorf A 1 Geffers R 3 von Boehmer H 1 Buer J Institutions 1 German Research Centre for Biotechnology 2 Hannover Medical School 3 Harvard Medical School Regulatory CD4 CD25 T cells T R control immune responsiveness to self and alloantigens Isolation and therapeutic manipulation of regulatory T cells requires the use of reliable surface receptors being selectively up regulated in T R cells Here we report a comparative expression profiling of polyclonal CD4 CD25 T R cells from wild type mice and monospecific CD4 T R obtained from hemagglutinin HA specific T cell receptor transgenic mice TCR HA crossed to mice expressing HA under control of the Igκ promotor Ig HA Out of 12500 genes analyzed we identified neuropilin 1 Nrp1 as a specific surface marker for CD4 CD25 T R cells Nrp1 a receptor involved in axon guidance angiogenesis and the activation of T cells is constitutively expressed on CD4 CD25 T R cells independent of their activation status On the contrary Nrp1 expression

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file83.shtml (2016-02-17)
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