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  • 17th International Mouse Genome Conference (2003)
    Insitute Co Authors 1 Song F 1 Kimura MT 1 Smith J 2 Matsuyama T 1 Nagase H Institutions 1 Roswell Park Cancer Insitute 2 RIKEN The Institute of Physical and Chemical Research Changes in DNA methylation during development suggest a role in development and tissue specific differentiation Restriction Landmark Genomic Scanning RLGS is a method for the 2 dimensional display of end labelled DNA restriction fragments When using a methylation sensitive restriction enzyme such as NotI as the restriction landmark RLGS primarily displays CpG islands and associated regions and can be used to detect developmental and tissue specific differences in DNA methylation Real RLGS was performed using DNA from several different mouse tissues C57Bl 6J to identify RLGS loci with tissue specific differences in DNA methylation status The genomic DNA sequences corresponding to these RLGS loci have been rapidly determined using new computational software that displays a virtual RLGS image of mouse genome sequence information The DNA sequence of 40 tissue specific RLGS loci were confirmed by PCR amplification of eluted Spot DNA using primers determined from the Virtual sequence The vast majority of the loci identified thus far are located within CpG islands 90 associated with the 5

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file84.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    and Multifactorial Trait Analysis Nutrition and Metabolic Disease Phenotyping Methods Imaging The Genetics and Genomics of Infectious Disease Verne Chapman Memorial Lecture Table of Contents Sponsor Exhibitor List Awards Photographs POSTER 37 CHEMICAL MUTAGENESIS OF MOUSE EMBRYONIC STEM CELLS Himmelbauer H Max Planck Institute of molecular Genetics Department of Vertebrate Genomics Berlin Dahlem Germany Co Authors Greber B Lehrach H Institutions Max Planck Institute of molecular Genetics Department of Vertebrate Genomics Berlin Dahlem Germany Despite the availability of mammalian genome sequences the functions of many genes remain unknown Mutant mice play an important role to study mammalian gene functions It would therefore be desirable to ultimately create a mutant for every gene in the mouse genome Most probably different complementary approaches will be required to achieve this goal Embryonic stem cell technology offers the possibility to manipulate the mouse genome and select for mutations in vitro Mutant animals can then be generated from selected clones We are inducing mutations chemically using ENU followed by the generation of mutant clone libraries in a 96 well format Such clone libraries can be specifically screened for mutations in genes of interest We are applying a protocol that allows the effective detection of mutations

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file85.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    2 Bruley C 6 Kolb H J 1 Wolf E Institutions 1 Institute of Molecular Animal Breeding and Biotechnology University of Munich 2 Institute of Cell Animal and Population Biology University of Edinburgh 3 FBN Dummerstorf 4 Institute of Endocrinology University of Tübingen 5 Lilly Deutschland Bad Homburg 6 Institute of Clinical Chemistry Munich Transgenic and knockout models have been used successfully in order to attribute specific functions to distinct growth factors Here we ask how in a complex organism expression of growth factors in fact is affected during altered growth are there general patterns of growth control which are found in different models or is growth control achieved by unique combinations of different expression levels of different growth factors These questions can ideally be answered by employment of mouse models generated via the phenotype driven approach through long term selection for high or low body weight Lines of mice selected for many generations for high H or low L growth in different laboratories have been collected We have studied systemic and local expression of growth relevant genes in 8 of these mouse lines highly diverging for body and carcass weights but also for nose rump lengths Serum IGF I

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file86.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Gack S 2 Schorpp Kistner M 3 Fürstenberger G 2 Hess J 1 Hahn M 2 Angel P 1 Lichter P Institutions 1 Division of Molecular Genetics 2 Division of Signal Transduction and Growth Control 3 Division Biochemistry of Tissue Specific Regulation Deutsches Krebsforschungs zentrum Im Neuenheimer Feld 280 D 69120 Heidelberg Germany The mouse skin model s of chemical carcinogenesis have been extensively used to investigate skin tumour initiation and promotion and the conversion of benign tumours papillomas to malignant squamous cell carcinomas SCC To provide reliable diagnostic markers and to develop novel therapeutic targets for cancer prevention and treatment an understanding of the molecular basis of tumourigenesis will be essential Particularly identification and functional characterization of cellular genes that are targeted by oncogenic stimuli represents an auspicious experimental approach For the studies of skin cancer modulation 7 12 dimethylbenzanthracene DMBA initiated and 12 O tetradecanoyl phorbol 13 acetate TPA promoted back skin of mice are used to identify tumour associated genes in the steps of initiation promotion and progression The different RNA samples derived from control and short term TPA treated skin papilloma and SCC biopsies were hybridised on two different arrays comprising 20K LION arrayTAG and 15K

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file87.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Analysis Nutrition and Metabolic Disease Phenotyping Methods Imaging The Genetics and Genomics of Infectious Disease Verne Chapman Memorial Lecture Table of Contents Sponsor Exhibitor List Awards Photographs POSTER 40 PHYSICAL LINKAGE OF SIMILARLY REGULATED GENES AND CONTEXT DEPENDENT REGULATION ON RIBOSOMAL PROTEIN GENES Imai K GSF National Research Center for Environment and Health Institute of Developmental Genetics Co Authors Wahl M Institutions GSF National Research Center for Environment and Health Institute of Developmental Genetics In recent years a huge amount of information on global gene expression profiles in diverse tissue types has been accumulating We have also carried out transcriptome analyses of chondrogenesis induced by BMP4 and of somitogenesis by using the method of SAGE Serial Analysis of Gene Expression Through analyses on our own data sets and also on those from others studies we realized two interesting possibilities 1 a significant fraction of downstream target genes regulated by a given signaling pathway may physically link in certain chromosomal domains 2 a characteristic pattern of up or down regulation of ribosomal protein genes may represent a status of cells or tissues in a certain biological context The former point suggests the presence of a yet unknown but somehow general mechanism

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file88.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    FINE HISTOLOGICAL GENE EXPRESSION MAP FOR THE FUNCTIONAL GENOME ANALYSIS Kusakabe M 1 ANB Tsukuba Institute 2 Institute for Animal Reproduction Co Authors Inoue J Aotsuka S Inoue T Matsuba K Institutions ANB Tsukuba Institute After the recent genome project was finished many researchers interests were moved to the functional genome analysis Although cDNA micro array and RT PCR methodologies are very sensitive to detect a lower level of gene expression they never show us in situ localization of mRNA Therefore it is very important not only to know which cell expresses a gene but also to know when and where they express a gene For this purpose in situ hybridization method can be useful tool Although the functional molecule is not always localized within the producing cell we can know the histological location of mRNA by in situ hybridization Therefore we have tried to develop the large scale in situ hybridization technique First we designed the temperature controllable metal block that installed a metal bar for agitating the hybridization solution Second we developed the supportive computer program for the probe preparation Then we made a Dig labeled cRNA probe by this method Finally in situ hybridization was carried out

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file89.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Genomics Research Group RIKEN GSC Co Authors 1 Toki H 1 Shimizu A 1 Nagano J 2 Gondo Y 1 Wakana S 1 Noda T 1 Shiroishi T Institutions 1 Mouse Functional Genomics Research Group RIKEN GSC 2 Population and Quantitative Genomics Team RIKEN GSC We have performed morphological screening for dominant phenotypes of adult mice in the ENU mutagenesis program in RIKEN GSC One of the targets is morphological abnormality in limb because limb development has been established as a good model system to study pattern formation such as axis formation and positional specification in vertebrates Detection for the primary abnormalities was achieved by the Modified SHIRPA screening protocol In the screening we added 18 tests to observe morphology to the original protocol We have detected various mutants that showed shortening of limbs joint and footpad anomalies M100005 and M100216 exhibited shortening limbs more severely in the hindlimbs than in the forelimbs In contrast M100413 and M100856 showed severe shortening in the forelimbs In addition to shortening of the humerus M100856 showed reduction in the radius ulna and hind limbs in the sever case M100856 was mapped to chromosome 15 M100451 showed absence of the middle phalanx in the

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file90.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    McKeone R MRC Harwell Co Authors Southwell A Cadman M Arkell R Davies J Bogani D Mallon A Weekes J Denny P Institutions MRC Harwell Parallel phenotype and genotype driven mutagenesis screens are being used in the functional annotation of the Del 13 Svea36H Del36H region of mouse chromosome 13 The phenotype driven screen uses mice with the Del36H deletion as a tool to uncover recessive ENU induced mutations Positional candidate cloning of these mutations requires full annotation of gene content and fine scale recombination mapping However mapping mutations was hampered because there were too few genetic markers within the Del36H region that were informative for strains involved in our crosses C57BL 6J BALB cAnN C3H HeH 101 H despite there being 12 000 mouse genetic markers 1 We therefore identified microsatellites in BAC sequences from the Del36H interval designed PCR assays and tested these for polymorphism by SSCP Seven of these markers and three MIT markers are being used for high throughput genotyping of mutant lines arising from a screen for lethal recessive mutations In order to recover additional alleles for selected genes in the Del36H interval an archive of DNA from the male F1 progeny of ENU mutagenised

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file91.shtml (2016-02-17)
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