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  • 17th International Mouse Genome Conference (2003)
    A COMPARATIVE PROTEOME TRANSCRIPTOME ANALYSIS OF GENES EXPRESSED IN LIVER AND KIDNEY OF THE MOUSE Mijalski T Drobyshev A Horsch M Schädler S Institute of Experimental Genetics GSF National Research Centre for Environment and Health Munich Germany Co Authors 2 Halder T 2 Harder A 3 Lottspeich L 1 Hrabe de Angelis M 1 JBeckers B Institutions 1 Institute of Experimental Genetics GSF National Research Centre for Environment and Health Munich Germany 2 TopLab GmbH Martinsried Germany 3 Max Planck Institute for Biochemistry Martinsried Germany So far little is known about the relationship between the transcriptional and post transcriptional regulation of gene expression in complex organisms Comparative transcriptome and proteome analyses in the mouse as mammalian model organism have not been performed We use DNA chip based RNA expression profiling and 2D gelelectrophoresis in collaboration with T H and A H to explore optimal conditions and the general feasibility of such comprehensive gene expression analyses To establish and optimise comparative gene expression techniques we made an assessment of RNA and protein profiles of adult liver and kidney in the mouse These tissues can be obtained as recoverable biological resource under reproducible conditions from inbred mouse strains We provide one of

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file92.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Authors 2 McWhir J 1 Horvat S Institutions 1 University of Ljubljana Biotechnical faculty Department of Animal Science Groblje 3 1230 Domzale Slovenia 2 Roslin Institute Division of Gene expression and Development EH25 9PS Roslin Midlothian Scotland UK Apoptosis and differentiation are tightly intertwined processes occurring at organ formation and remodelling during embryonic development Raidd a dual domain adaptor protein has been shown to mediate the recruitment of Caspase 2 to tumour necrosis factor receptor 1 TNFR 1 signalling complex through RIP kinase So far Raidd transcripts of 1 4 kb and 1 8 kb have been detected by Northern analysis in whole embryos at some stages in the foetal liver and in most adult tissues but a detailed analysis of the tissue specific pattern of expression has not yet been reported Raidd overexpression studies suggest that apart from the established role in the cascade of apoptotic pathway Raidd may have additional function during development To elucidate the role of Raidd during mouse embryogenesis we examined Raidd expression pattern in midgestation mouse embryos where the processes of organogenesis are most dynamic In the study we utilized our previously established Raidd mouse line carrying a reporter transgene Beta galactosidase β gal

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file93.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    for Experimental Cancer Research ISREC Co Authors Guichard S Beermann F Institutions Swiss Institute for Experimental Cancer Research ISREC Pigment cells are an attractive system to understand the molecular mechanism of cell lineage commitment They are not essential for viability and they display visible phenotypes pigmentation In mammals pigment cells originate from two different lineages melanocytes of skin hair follicle and uveal tract of the eye originate from the neural crest whereas cells of the retinal pigmented epithelium RPE originate from the optic cup of the developing forebrain Three pigment cell specific enzymes forming the tyrosinase related protein family are responsible for the melanin production tyrosinase TYRP I and DCT Within the mouse tyrosinase regulatory region a S MAR containing 3 6 kb enhancer fragment was identified at 15 kb from the transcriptional start site which was shown to behave as a locus control region LCR insulating the transgene from position effects Previous experiments performed in our lab using lacZ reporter in transgenics have pointed out the following enhancer features the enhancer is necessary to drive detectable expression in melanocytes whereas the tyrosinase promoter alone is sufficient in RPE cells the enhancer does not increase transgene expression in RPE We

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file94.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Marra M 3 Abe K and 4 Roe B Institutions 1 National Institute on Aging NIH Baltimore U S A 2 Canada s Michael Smith Genome Science Center Vancouver BC Canada 3 Riken Tsukuba Institute Ibaraki 305 0074 Japan 4 Advanced Center for Genome Technology University of Oklahoma Oklahoma U S A Many embryonic lethal mutations and genes associated with stemness and growth control fall in the t complex on mouse chromosome 17 The challenge of analyzing this region and identifying genes bearing lethal mutations is increased because of the duplications and four large segments that undergo inversion To facilitate the analysis in conjunction with mouse genome sequencing efforts a BAC STS physical map was assembled in large portions of the region and 43 BACs were sequenced Sixteen of the estimated 30 Mb region has been assembled in 3 contigs and integrates BAC clones recovered by screening with STSs and supported by fingerprint data The information confirms much of the genome sequence assembled from other sources and adds coverage of segments not yet recovered Contig 1 8 0 Mb extending from MGD cM 4 0 to 8 3 with 188 BACs formatted with 166 STSs includes markers Brachyury at the

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file95.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    1 Kimura MT 1 Conroy JM 1 Nowak N 1 Igarashi J 2 Yoshiki A 3 Kusakabe M Institutions 1 Roswell Park Cancer Institute 2 RIKEN BioResource Center 3 ANB Tsukuba Institute We have presented the heavy ion beam HIB approach of mutagenesis in previous IMGC meetings There are unique advantages for HIB mutagenesis Firstly it uses a relatively low dose localized irradiation targeted specifically to the testis Secondly the mutation rate as measured by the increased frequency of functional mutations e g using HPRT is lower than ENU mutagenesis The frequency with which mutant phenotypes are produced is however similar Thirdly the mutations that are induced are mainly small deletions which result from clustered double strand DNA damage Goodhead 1994 Rydberg 1996 This is an important aspect of HIB since it is subsequently relatively easy to detect deletions using molecular genome scanning approaches We collaborated with the Microarray Genome Resource Core at RPCI and have recently developed mouse high resolution BAC CGH array Ligation mediated PCR was employed to generate representations of currently 2 500 mouse BACs for printing with high reproducibility and provided essentially identical ratios as those generated using plasmid DNA from the same BACs Details concerning

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file96.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    DETECTING Γ H2AX FOCI IN CB 17 MOUSE PERIPHERAL LYMPHOCYTES ORGAN TISSUES AND SCID LYMPHOMA CELLS AFTER 137 CS Γ RAY IRRADIATION Nakajima H Osaka University Co Authors Tsuboi R Nomura T Institutions Osaka University To measure the radiation damage γ H2AX foci in the irradiated CB 17 peripheral lymphocytes organ tissues and SCID lymphoma cell line established from CB 17 scid scid mice were detected as fluorescent signals since phosphorylation of H2AX by PI3 kinase at serine 139 γ H2AX occurs following DNA double strand but not single strand break induction and is probably the earliest manifestation of the lesion CB 17 mice and SCID lymphoma cells were exposed to 0 0 1 0 2 0 5 1 2 4 Gy 1 Gy min of γ rays The γ H2AX foci were detected as fluorescent signals in both cells at 3 minutes after irradiation and the signal intensity reached maximum within 30 minutes after exposure and then faded quickly Dose dependent signal amplification was also observed These results suggest that quantitation of DNA double strand breaks DSBs by the γ H2AX foci is an useful biodosimeter for detecting high and low dose radiation damage in mice It is also

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file97.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    J N 3 Beechey C V 1 Choi J D Institutions 1 Department of Medical molecular Genetics GKT School of Medicine London SE1 9RT UK 2 Division of Human Genetics The Children s Hospital of Philadelphia PA 19104 USA 3 Mammalian Genetics Unit Harwell Didcot Oxon OX11 ORD UK Imprinted genes are differentially expressed from the maternally and paternally inherited alleles Accordingly uniparental inheritance of an imprinted chromosome or region leads to the mis expression of imprinted genes Mouse stocks carrying Robertsonian and reciprocal translocations for chromosomes 7 11 18 have generated embryos with uniparental disomy or partial chromosome uniparental duplications RNAs from these mice have been used to query DNA microarrays A computer based screen has reduced false positives by map location to provide a systematic approach to novel imprinted gene identification 1 Most of the known imprinted genes are readily detected demonstrating proof of concept of this approach 2 a number of novel imprinted genes have been identified 3 two novel imprinted gene clusters have been detected These regions contain a classically imprinted fingerprint with alternative transcripts CpG islands and direct repeats Detailed sequence and epigenetic analyses have been useful in defining hallmark features of imprinted genes We

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file98.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    List Awards Photographs POSTER 51 COMPARATIVE AND FUNCTIONAL CHARACTERIZATION OF THE PHR1 LOCUS Peterson K The Jackson Laboratory Co Authors Burgess R Johnson M Roix J Welsh I O Brien T Institutions The Jackson Laboratory Chromosomal deficiencies provide a platform for uncovering developmentally important phenotypes We have used a set of overlapping deletions surrounding the piebald locus on distal mouse chromosome 14 to perform complementation analysis to define a minimal critical region associated with respiratory distress and lethality at birth We have cloned and characterized the candidate gene Phr1 that is located within the 480 kb respiratory distress interval Phr1 is the ortholog of the human protein associated with myc P AM and the homolog of Drosophila highwire h iw and C elegans regulator of presynaptic morphology r pm 1 The Phr1 gene contains 85 exons and encodes a 4708 aa protein that shares 96 identity with human Pam Phr1 is dynamically expressed throughout the embryonic and postnatal nervous system The phrenic nerve of the piebald deletion respiratory distress mutants fails to completely innervate the diaphragm and nerve terminal morphology is disrupted similar to synaptic defects seen in fly and worm mutants These findings suggest an evolutionarily conserved role for

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file99.shtml (2016-02-17)
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