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  • 17th International Mouse Genome Conference (2003)
    of Biochemistry and Biotechnology Technical University of Braunschweig Spielmannstrasse 7 D 38106 Braunschweig Co Authors Prochnow B R Arnold HH Institutions Department of Cell Molecular Biology Institute of Biochemistry and Biotechnology Technical University of Braunschweig Spielmannstrasse 7 D 38106 Braunschweig Krueppel associated box KRAB zinc finger proteins ZFPs are known to function as transcriptional repressors with the KRAB domain being responsible for protein protein interactions and the C2H2 type zinc fingers mediating DNA binding Although the function of only a few KRAB ZFPs is known they appear to play important roles during cell differentiation and development Here a novel KRAB ZFP was identified by gene trapping The full length cDNA of 4043 bp contains an open reading frame of 2055 bp The putative protein has both KRAB A and KRAB B boxes at the N terminus and 10 C2H2 zinc finger motifs at the C terminus LacZ expression of the trapped gene is found in all embryonic stages analyzed from E9 5 to E17 5 with a general salt and pepper staining RT PCR also revealed transcripts in embryos of E11 3 E13 5 in postnatal skin P0 P9 as well as in most adult organs with the exception of

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file116.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    22 Suehiro cho Tsurumi ku Yokohama Kanagawa 230 0045 Japan and Genome Science Laboratory RIKEN Wako main campus Hirosawa 2 1 Wako Japan Co Authors Shiraki T Waki K Kondo S Ishizuka Y Arakawa T Kawai J Hayashizaki Y Carninci P Institutions Laboratory for Genome Exploration Research Group RIKEN Genomic Sciences Center GSC Yokohama Institute 1 7 22 Suehiro cho Tsurumi ku Yokohama Kanagawa 230 0045 Japan and Genome Science Laboratory RIKEN Wako main campus Hirosawa 2 1 Wako Japan Alternative splicing has key role in diversification of the proteome especially of considering that the diversity of mammalian should be achieved with only about 30 000 protein coding genes Although alternative splicing is known we have not yet gained a comprehensive view on this phenomena To address this problem we have developed a new type of libraries ASL alternative splicing libraries by fishing out alternatively spliced exons from two different mouse full length cDNA libraries Subsequent sequencing of these libraries produced a new type of sequence tag ASSETS Alternative Splicing Sequence TagS ASSETS are enriched in alternative splicing exons their upstream and downstream exon sequences and identify splicing junctions In this time we prepared the ASL from melanocyte and melanoma

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file117.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    A J Division of Medical Genetics GKT School of Medicine 8 th Floor Guy s Tower London SE1 9RT UK Co Authors 2 Underkoffler L A 2 Collins J N 1 Choi J D 3 Beechey C V 1 Oakey R J Institutions 1 Division of Medical Genetics GKT School of Medicine 8 th Floor Guy s Tower London SE1 9RT UK 2 Division of Human Genetics The Children s Hospital of Philadelphia USA 3 Mammalian Genetics Unit Harwell Didcot Oxon OX11 ORD UK Imprinted genes are differentially expressed from the maternally and paternally inherited alleles Accordingly uniparental inheritance of an imprinted chromosome or region leads to the mis expression of imprinted genes DNA microarrays have identified a novel imprinted gene from a uniparentally duplicated region of mouse chromosome 7 expressed mainly in brain referred to hereafter as 575 Inter subspecies allele specific assays demonstrate that 575 is imprinted in brain and biallelically expressed in non brain tissues The imprinted transcript size is approximately 3 7kb and the non imprinted version is 4 0kb Northern blot analysis confirmed the presence of both transcripts in the brain but the 3 7kb species was not detected in any of the other tissues

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file118.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    P 1 Parkinson N 1 Willan J 1 Pritchard C 1 Underhill P 1 Hilton H 1 Madge J 1 Polley S 2 Carey AH 2 Jones H 3 Soares B 1 Brown S Institutions 1 Medical Research Council Harwell UK 2 Oxagen Ltd UK 3 University of Iowa USA We have used cDNA microarrays to explore gene expression profile changes accompanying the development of the sensory neuroepithelium the organ of Corti OC in the inner ear of deaf mouse mutations at P1 and P3 which is one of the crucial periods for sensory hair cell differentiation Of 6700 random genes arrayed from a normalised newborn mouse inner ear NMIE cDNA library we found the expression level of 364 genes increased 2 fold or more and 90 genes decreased at least 2 fold during normal OC development from P1 to P3 In comparison the OC of the whirler mice a mutant that shows abnormal elongation and development of stereocilia shows 184 genes increased and 219 decreased between P1 and P3 The developmental expression pattern of several clusters of genes in the mutant OC does not follow that of wild type wt 29 genes whose expression level increased significantly between P1

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file119.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Authors 1 Sirohzu H 1 4 Purbowasito W 3 Toyoda A 3 4 Kato R 1 Suda C 5 Hori T 6 Tsudzuki M 7 Matsuda Y 8 Mizuno S 3 Hattori M 9 Mukai T 3 10 Sakaki Y 1 2 Sasaki H Institutions 1 National Institute of Genetics 2 Graduated University for Advanced Studies 3 RIKEN 4 Kyushu University 5 Kinki University 6 Hiroshima University 7 Hokkaido University 8 Nihon University 9 Saga Medical School 10 The University of Tokyo Previous studies revealed that IGF2 and MPR IGF2R are imprinted in eutherian mammals but not in birds IGF2 lies in a large imprinted cluster in mice and humans and its imprinting is regulated by long range mechanisms As a step to understand how the imprinted cluster evolved and how it is regulated we determined and compared a 582 kb mouse sequence containing Mash2 Igf2 and H19 and a 490 kb chicken sequence of the orthologouse region We found that most of the genes were conserved between the two species maintaining the same transcriptional polarities and exon intron structures However H19 an imprinted gene adjacent to Igf2 was missing in chicken Chicken CASH4 and INS the orthologuse of imprinted

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file120.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Abe K GSF National Research Centre for Environment and Health Co Authors 1 Wagner S 2 Kalaydijev S 1 3 Jakob T 1 Soewarto D 1 Fuchs H 1 Hrabe de Angelis M Institutions 1 GSF National Research Centre for Environment and Health 2 Institute of Medical Microbiology TU München 3 Division of Environmental Dermatology and Allergy TU München Rheumatoid arthritis RA is a multifactorial disease involving inflammatory conditions that lead to joint destruction Despite tremendous efforts in the field the etiology and molecular pathogenesis of RA remain largely unknown Therefore animal models of arthritis provide important tools for the dissection of the molecular mechanisms leading to RA The dominant mutation Abnormal limb 18 Ali18 was isolated from the Munich ENU mutagenesis screen in the mouse Ali18 homozygous mice display destruction of fingers by severe swelling at adult stages but have normal lifespan and fertility Histological examinations revealed that many tissues including joints were destroyed by inflammatory infiltrate that consists of polymorphnuclear leukocytes and lymphocytes To elucidate how the immune system concerns this phenotype we analyzed various immunological parameters in peripheral blood of Ali18 mice by ELISA and flow cytometry Increased B cell populations and elevated levels of antibodies against

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file121.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Chrobot N 2 Rubinsztein D C 1 Brown S D Institutions 1 MRC Mammalian Genetics Unit and UK Mouse Genome Centre 2 Cambridge Institute for Medical Research Huntington s disease HD is an autosomal dominant progressive neurodegenerative disorder caused by an expanded polyglutamine PolyQ repeat in exon 1 of the HD gene It is characterized by motor cognitive and psychiatric symptoms and death ensues about 15 years after disease onset The number of PolyQ repeats is polymorphic in humans with normal individuals having up to 37 glutamines whereas disease is associated with more than 38 repeats About 70 of the variance of the age at onset can be accounted for by its inverse relationship with PolyQ tract length Although HD is caused by an expanded PolyQ tract little is known about the downstream effects of the mutation and the possible genetic modifiers that may modulate either the severity or the onset of the disease In order to try to identify HD phenotype modifier genes we have initiated a genetic modifier screen using ENU mutagenesis with a mouse model of HD In order to select the most suitable model for the ENU screen we characterized two different widely available transgenic models

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file122.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Moorwood K 2 Brown K 2 Malik K 1 Ward A Institutions 1 University of Bath 2 University of Bristol WT1 is a phylogenetically conserved nuclear protein essential for a variety of developmental processes affecting both transcriptional regulation and RNA metabolism To understand the mechanisms of WT1 regulation in vivo experiments are crucial because the complex and strict developmental expression pattern of WT1 is not accounted for by the transcriptional

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file123.shtml (2016-02-17)
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