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  • 17th International Mouse Genome Conference (2003)
    DEVELOPMENT Zhao S TIGR BAC Ends Team The Institute for Genomic Research TIGR Co Authors Shetty J Shattsman S Institutions TIGR BAC Ends Team The Institute for Genomic Research TIGR Using paired BAC end sequence BES matches we have mapped 200 000 human mouse and rat BACs to the genome of each of the three species Over 99 of the clones were placed to unique positions on each genome forming contigs up to 50Mb covering 90 of the genome with 6X coverage and a few small gaps Over 95 of the genes have their exons and additional 50kb upstream and downstream regions covered in a single BAC or BAC contigs providing an important resource for gene functional studies Genome duplications create difficulties in assembly and are often involved in evolution and diseases With the 1 of the BACs that were mapped to multiple places on the genomes we have found that 5 of the human genome 7 of the rat genome and 1 of the mouse genome were involved in intra chromosomal or inter chromosomal duplications Most of these regions were repeats rich but some also encoded genes We are analyzing these genes and repeats to understand their roles in

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file203.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Co Authors 1 Gregorova S 1 Divina P 2 Buckiová D 1 Landíková M 1 Forejt J Institutions 1 Institute of Molecular Genetics AS CR and Centre of Integrated Genomics 2 Institute of Experimental Medicine AS CR Hybrid sterility is a postzygotic reproductive isolation mechanism which appears in the early stages of speciation of various species In the house mouse Mus musculus musculus and Mus musculus domesticus represent recently separated mouse subspecies particularly suitable for genetic studies of hybrid sterility since the reproductive barrier between them is still incomplete Here we introgressed the X chromosome of the PWD Ph inbred strain derived from M m musculus wild mice into the genetic background of C57BL 6J inbred strain predominantly of M m domesticus origin The resulting B6 X PWD X B6 females were viable and fertile but B6 X PWD Y B6 males were completely sterile The hybrid sterility is associated with reduced testes weight a limited apoptosis of spermatocytes lower sperm count and spermatozoa with abnormal heads The QTL analysis revealed the oligogenic nature of sterility the central and distal parts of the X chromosome were associated with reduced testes weight and the lower sperm count while the central part

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file204.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    TISSUE Aksu S Research Institute for the Biology of Farm Animals Dummerstorf Co Authors 2 Neuschl C 1 Renne U 3 Koczan D 3 Thiesen H J 1 Brockmann GA Institutions 1 Research Institute for the Biology of Farm Animals Dummerstorf 2 Institute of Cell Biology University of Rostock 3 Institute for Immunology University of Rostock Obesity is a major health risk factor for several kinds of serious diseases and metabolic disorders In this study we used DU6i and DU6 mouse lines which have been selected for high body weight DU6 mice have the highest body weight known worldwide DU6i is an inbred derivate of DU6 It is twice as heavy and three times as fat as unselected control mice In addition the selected mice are characterized by highly increased serum concentrations of leptin insulin and IGF 1 QTL mapping analyses in crosses between selected and non selected control lines showed the presence of different QTLs that affect body weight obesity and muscle weight as well as serum concentrations of biological molecules and hormones on several different chromosomes Microarray expression analyses showed that 88 genes are differentially expressed in fat tissues between the selected and non selected mouse lines 14

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file205.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Angel J M The University of Texas MD Anderson Cancer Center Science Park Research Division Co Authors Riggs P K Caballero M DiGiovanni J Institutions The University of Texas MD Anderson Cancer Center Science Park Research Division Cancer susceptibility in the general population is a function of multiple poorly penetrant modifier genes each of which contributes to but is not solely responsible for predisposition to developing a particular type of cancer after exposure to certain environmental carcinogenic agents Genetic differences in susceptibility to two stage skin carcinogenesis have been known for many years and the major contribution to susceptibility appears to be at the level of tumor promotion Studies suggest that susceptibility to 12 O tetradecanoylphorbol 13 acetate TPA skin tumor promotion is a multigenic trait Loci that modify the susceptibility to TPA skin tumor promotion have been mapped to chromosomes 1 2 9 and 19 in genetic crosses of sensitive DBA 2 with relatively resistant C57BL 6 mice One promotion susceptibility locus Psl1 was mapped to an 40 cM region of distal chromosome 9 Results from tumor studies using interval specific congenic mouse strains suggest that at least two genes that modify the response to TPA skin tumor promotion

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file206.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    R 1 Bellier S 1 Salaün P 3 Montagutelli X 1 Panthier J J Institutions 1 INRA Maisons Alfort 2 Present organisation MRC Edinburgh 3 Institut Pasteur Paris PRM Alf inbred mice exhibit a selective intestinal lengthening Indeed PRM Alf intestine is one third longer compared to other inbred strains C57BL6 J C3H He DBA 2J and 129S2 The phenotype is acquired mostly during the postnatal period before weaning Interestingly the gastrointestinal transit time as evaluated using thermophilic Bacillus subtilis spores as an inert marker is identical in PRM Alf and other inbred mice thus implying an increase in transit speed Physiologic analysis showed that mechanical contractions and slow wave activity are increased in PRM Alf proximal small intestine compared to controls Since the intestinal electrical pacemaker activity originates in the interstitial cells of Cajal ICC the distribution of ICC in small intestine and colon segments of PRM Alf mice and controls was analysed We found that the ICC number is increased in small intestine and colon of PRM Alf mice compared to controls We studied the genetic determinism of intestine lengthening in PRM Alf We found that it is polygenic It also involves a strong maternal effect Cross fostering

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file207.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    1 Puma C 2 Singer J 2 Lander E 3 Hill A 3 Nadeau J 1 Drazen J M Institutions 1 Brigham and Women s Hospital and Harvard Medical School Boston MA 2 Whitehead Insitute MIT Cambridge MA 3 Case Western Reserve University School of Medicine Cleveland OH Interstrain variability of naïve airway hyperresponsiveness AHR provides an important tool for genetic analysis of loci contributing to this trait which is a component of human asthma A phenotype driven strategy was used to select congenic mice from a serial backcross of hyperresponsive A J mice to B6 mice Genotyping of N7 hyperresponsive males revealed 3 retained A J regions on chromosomes 2 6 and 10 To test the causal association of the retained regions hyperresponsive N7 males were backcrossed to create an expanded N8 population QTL analysis of over 120 unselected N8 males did not identify a significant association of any single locus with AHR However when the data was analyzed to account for interactions a highly significant association was found for inheritance of A J alleles on chromosomes 2 and 6 together Lod 4 93 Chromosome substitution strains CSS were used to independently evaluate these results Strains heterozygous for A

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file208.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    BUILDING A COMMUNITY RESOURCE AND INTEGRAL TOOL FOR COMPLEX PHENOTYPE ANALYSIS Bogue M The Jackson Laboratory Co Authors Marcus J Grubb S Institutions The Jackson Laboratory Comprehensive characterization of inbred strains is needed to identify new mouse models and to choose optimal strains for breeding strategies and other techniques leading to the identification of genomic components contributing to complex phenotypes The Mouse Phenome Project is an international and ongoing collaborative effort to promote the quantitative phenotypic characterization of a defined set of inbred mouse strains under standardized conditions and to make the data publicly available With the guidance of an International Steering Committee and participation of the research community we have coordinated efforts to choose an optimal set of 40 inbred strains and we are interested in collecting data from these strains and their reproducible derivatives F1 hybrids RI lines consomics congenics Collectively this reference population represents virtually unlimited but reproducible combinations of a set of fixed alleles from the original 40 strains Members of the research community are contributing valuable datasets for inclusion in the Mouse Phenome Database MPD www jax org phenome There are about 500 measurements and 450K SNPs currently available with a number of new initiatives

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file209.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    OF THE MOUSE LINE DU6I Brockmann GA Research Institute for the Biology of Farm Animals Dummerstorf Germany Co Authors Bevova M R 1 Aksu S 2 Aulchenko J 1 Renne U Institutions 1 Research Institute for the Biology of Farm Animals Dummerstorf Germany 2 Erasmus University of Rotterdam Netherlands The mouse line DU6i is a model for polygenic growth and obesity which is an inbred derivate from the line DU6 selected over 100 generation for the high body weight at the age of 42 days So far many QTLs have been identified in the intercrosses DU6 x DUKs and DU6i x DBA 2 Brockmann et al 1998 2000 2001 Evidence was found that loci having small direct effect on growth or obesity contributed to the obese phenotype by gene gene interaction For more precise characterisation of effects of single QTLs and targeted analysis of gene gene interaction we generated chromosome substitution strains CSS for DU6i mice CSS were constructed by recurrent backcrossing of DU6i mice to the commercial inbred line DBA 2 as recipient line The transfer of the chromosome of interest and the genetic background of the recipient line was controlled by 148 genetic markers Here we present results

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file210.shtml (2016-02-17)
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