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  • 17th International Mouse Genome Conference (2003)
    TUMORAL EFFECT OF HYSTONE DEACETYLASE INHIBITORS HDACIS IN A GAMMA IRRADIATED MOUSE LYMPHOMA MODEL Herranz M Spanish National Cancer Centre Co Authors de la Cueva E Villar Garea A Fraga MF Martín Caballero J Esteller M Institutions Spanish National Cancer Centre Pharmacological manipulation of chromatin remodelling process by histone deacetylase HDAC inhibitors might develop into a potent and specific strategy for the treatment of cancer Alterations in histone acetylation may lead to changes in chromatin structure and transcriptional deregulation of genes A protocol to induce thymic lymphomas in mice has been developed by gamma irradiation Gamma irradiation in a whole body way induces thymic lymphomas in 90 of treated mice from C57BL 6J strain Our goal is to analyze the effect of hystone deacetylase inhibitors in the tumor initiation and progression One hundred animals were irradiated and nine hystone deacetilases inhibitors assays were evaluated Intraperitoneal inoculation of six drugs Valproate Butyrate 2 different doses TSA SAHA LAQ 2 different doses and MS275 was performed 10 animals each 5 males and 5 females and one control assay PBS Phophate Buffer Saline inoculation Each mouse was imaged with a periodicity of 2 weeks after the establishment of the treatment using conventional T2

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file243.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    van Ree J 1 Adan RAH 2 van Lith H 2 van Oost BA 1 Burbach J Institutions 1 Rudolf Magnus Institute of Neuroscience University Medical Centre Utrecht The Netherlands 2 Veterinary Medicine University of Utrecht The Netherlands 3 Department of Psychopharmacology University of Utrecht The Netherlands The genetic dissection of integrated behaviours requires refinement of behavioural phenotypes The display and analysis of refined behavioural components have to be established by specific environmental conditions that elicit ethological needs At present behavioural studies are mainly conducted in short lasting dedicated tasks thereby ignoring behaviour normally displayed in the home cage Therefore we constructed an enriched home cage that includes the possibility to provide auditory and visual stimuli at a certain time or when the animal is at a certain place Longitudinal continuous behavioural observations in this cage are based on the automated analysis of video images Noldus Information Technology The Netherlands and permit differentiation between stimulus induced specific behaviours baseline and novelty behavioural patterns and rhythmicity Moreover it prevents the confounding influence of the effect of human interference such as transport on behavioural characteristics So far differences between inbred mice strains could be distinguished by analyzing features of their locomotor patterns

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file244.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    Maximilian University of Munich Co Authors 1 Holupirek E 1 Rathkolb B 2 Gailus Durner V 2 Fuchs H 2 Hrabe de Angelis M 1 Wolf E Institutions 1 Ludwig Maximilian University of Munich 2 GSF National Research Center for Environment and Health Institut of Experimental Genetics Munich The clinical chemical screen of the German Mouse Clinic identifies and characterises mouse mutants with defects of various organ systems or changes in metabolic pathways asociated with aberrations of clinical chemical parameters or hematological parameters and blood coagulation to model human diseases like diabetes mellitus anemia coagulopathies and others The methods used are appropriate routine procedures allowing the screening of large numbers of mice for a broad spectrum of parameters In the primary clinical chemical screen 19 different parameters are measured including various enzyme activities as well as plasma concentrations of specific substrates and electrolytes The high throughput of the screen is insured by the use of a Olympus AU 400 autoanalyzer Additionally we measure 8 basic hematological parameter with a blood analyzer ABC Animal Blood Counter Scil which has been carefully validated for the analysis of mouse blood In a secondary screen specific profiles of organs are performed if certain parameters

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file245.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    of Infectious Disease Verne Chapman Memorial Lecture Table of Contents Sponsor Exhibitor List Awards Photographs POSTER 198 A NEW METHOD FOR CONDITIONAL IMMORTALIZATION OF CELLS FROM MOUSE MUTANTS May T GBF Gesellschaft fuer Biotechnologische Forschung Co Authors Hauser H Wirth D Institutions GBF Gesellschaft fuer Biotechnologische Forschung Primary cells derived from mouse mutants or knock outs represent an important tool for a detailed molecular characterization of the respective phenotype Protocols to reproducibly immortalize cells of different tissues would be of great importance In particular a conditional reversible immortalization would avoid any interference of the immortalizing gene with cellular signalling pathways We developed a vector system employing the tet on system that allows the conditional immortalization of primary cells The vector is autoregulatory and the expression of the transactivator as well as the expression of the immortalizing gene e g SV40 large T antigen depends on doxycyclin Thus the immortalization process should be reversible by removing doxycyclin from the cells As a proof of principle we have immortalized mouse embryo fibroblasts by these vectors and characterized the resulting clones These clones showed highly regulated expression of the oncogene which led to a doxycylin dependent immortal phenotype The reversibility was monitored by

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file246.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    BEHAVIOR Miller D Oak Ridge National Laboratory Oak Ridge TN Co Authors 1 3 Rinchik E M 3 Goss K 1 Johnson D 1 3 Snoddy J 3 Galloway L 4 Ferkin M 2 Hamre K 4 Matthews D 4 Mittleman G 2 Jablonski M 2 Williams R 2 Swanson D 2 Goldowitz D and the Tennessee Mouse Genome Consortium Institutions 1 Oak Ridge National Laboratory Oak Ridge TN 2 University of Tennessee Health Sciences Center Memphis TN 3 University of Tennessee Knoxville TN 4 University of Memphis Memphis TN The statewide mutagenesis and phenotyping effort by TMGC Neuromutagenesis Project has now completed year 3 This project s goal is to generate new mutations in genes that control the development and function of the nervous system and to develop and apply breeding and screening protocols that allow the ascertainment of quantitative statistically sensitive or otherwise difficult or complex neurobehavioral phenotypes and to set aside an aging colony for identifying late onset recessive effects The TMGC Neuromutagenesis Project s main web site http tnmouse org displays our currently available mutants ascertained from a variety of tests spread into various phenotyping domains Reporting and analysis of test results via MuTrack our internal web based database is also available to the outside community for individuals interested in viewing raw data To date 194 have been screened by all domains except aging across the entire TMGC and 1 192 have been evaluated for lethal and visible mutations So far 73 mutations have been identified with a number of others in the more subtle eye neurohistology ethanol and general behavior domains Some of the neurological phenotypes include abnormal brain histology deficiencies in cerebellar morphology and function abnormal retinal phenotypes and locomotor activity variants and many others New this year also was the initiation of re

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file247.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    FOR NEUROLOGICAL MUTATIONS AT THE JAX NEUROSCIENCE MUTAGENESIS FACILITY NMF Pretel S The Jackson Laboratory Co Authors Frankel WN Seburn KL and the JAX NMF Staff Institutions The Jackson Laboratory The Neuroscience Mutagenesis Facility at The Jackson Laboratory was established to produce mutant mice that may serve as experimental models for human neurological disease These mutants are produced by injecting C57BL 6J mice with N ethyl N nitrosourea and identified through genome wide phenotypic screening of third generation G3 families The screens used are focused on the examination of visual auditory vestibular gustatory olfactory and metabolic functions seizure threshold and growth and development progression of G3 families and include behavioral observations and histological procedures G3 families that show abnormal screen results are selectively mated and once heritability of the observed abnormal behavior has been confirmed the affected mice are regarded as mutants The animals are then further tested to characterize the observed abnormalities Using this process 21 mutants mouse lines have been established during the last two years The majority 80 of observed mutations is recessive and includes abnormal functions of motor and neuromuscular n 13 visual n 3 auditory and vestibular systems n 1 metabolism n 1 or abnormal

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file248.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    POSTER 201 GENETIC ANALYSIS OF HOST SUSEPTIBILITY TO CARDIOVIRULENT COXSACKIE VIRUS B3 INFECTION Aly M University of Ottawa Co Authors Vidal S Institutions University of Ottawa Coxsackie virus B3 CVB3 is responsible of 50 of viral myocarditis cases in North America Differences in disease severity caused by a given strain of CVB3 are thought to be largely related to host genetic factors for which little is known Infection of mice with CVB3 mimic human heterotypic responses providing a useful model to identify the host genetic determinants of viral myocarditis To identify those genes and further understand the molecular mechanisms of the disease progression we are taking advantage of naturally occurring variability among inbred strains for the genetic analysis of three CVB3 induced phenotypes viral load in target organs weight loss percentage and heart histopathology A new modified Triangle background algorithm was developed to quantify heart histopathology from florescent damaged cardiomyocytes images An F 2 intercross was constructed using inbred A and B10 A mice as progenitors which display resistance and sensitivity respectively to CVB3 induced myocarditis Our statistical analysis for weight loss percentage and heart histopathology indicated that the Pearson s correlation coefficient for these traits is highly significant In

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file249.shtml (2016-02-17)
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  • 17th International Mouse Genome Conference (2003)
    UK Mouse Genome Centre Mammalian Genetics Unit Harwell UK Co Authors 1 Haynes A 1 Moyes S 1 Hopes E 2 Gingles N 3 Broman K 5 McPheat W 4 Morten J 2 Alexander J 2 Andrew P 1 Brown S 1 Denny P Institutions 2 University of Leicester UK 3 John Hopkins University USA 4 AstraZeneca UK 5 AstraZeneca Sweden The pneumococcus Streptococcus pneumoniae is an important human pathogen causing pneumonia bacteremia and meningitis and associated with very high morbidity and mortality It is likely that host genetic factors play a significant role in susceptibility to pneumococcal disease as is clearly the case for other infectious diseases such as malaria and tuberculosis However genetic linkage analysis is impractical in pneumococcal disease due to the paucity of sibling cases or multiple case families As an alternative approach to the identification of candidate disease genes we have developed a murine model of genetic susceptibility to pneumococcal infection Nine inbred strains of mice were infected intranasally with Streptococcus pneumoniae D39 and BALB c and CBA Ca were found to be resistant and susceptible respectively A genome scan for loci responsible for this difference in susceptibility to invasive infection was conducted in the

    Original URL path: http://www.imgs.org/Archive/abstracts/2003abstracts/file250.shtml (2016-02-17)
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