archive-org.com » ORG » I » IMGS.ORG

Total: 854

Choose link from "Titles, links and description words view":

Or switch to "Titles and links view".
  • The 16th International Mouse Genome Conference (2002)
    Y RadGenomics Project Frontier Research Center National Institute of Radiological Sciences Clinical radiobiology has been stimulated by the search for predictive assays to determine the intrinsic tumor or normal cellular radiosensitivity of patients with aim to individualize clinical radiotherapy In order to achieve this purpose laboratory mice are very important to identify some genes which are related to the radiosensitivity The comet assay is an excellent procedure to study DNA damage and its repair of eucaryote cells Recently the multi lane comet assay method was originally developed with the aim of the high throughput comet assay Our new method can analyse six samples simultaneously on one slide and also minimize experimental errors between slides observed in conventional comet assay The whole blood was collected from mice of four inbred strains C3H HeMs C57BL 6J A J and STS A and of two congenic strains C3H scid and C B 17 scid These samples were irradiated with 0 2 4 and 8 Gy to analyse initial DNA damage For DNA repair studies the samples were incubated at 37 ºC after irradiation of 4 Gy and were analysed at 5 15 and 30 minutes by multi lane comet assay For initial damage

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file8.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    Hillebrandt Department of Medicine III University Aachen Lammert F University Aachen Background Host genetic factors are likely to contribute to the variable course of hepatic fibrosis in response to chronic liver injury Our aim was to identify unknown susceptibility loci for hepatic fibrosis in a cross between fibrosis susceptible and resistant inbred mice Methods Seven inbred mouse strains were treated with CCl4 and hepatic fibrosis was phenotypically characterized by histology and hepatic hydroxyproline levels Quantitative trait loci were identified in the intercross between susceptible BALB cJ and resistant A J mice by genome wide scans and by in silico haplotype mapping with 109 SNPs covering the whole genome Results In this model marked strain differences in fibrosis susceptibility exist with BALB c mice being most susceptible QTL analysis identifies susceptibility loci on chromosomes 2 and 15 that significantly affect the stage of fibrosis and hydroxyproline levels These loci are called Hfib1 and Hfib2 for hepatic fibrogenic genes 1 and 2 Hfib1 is defined by genetic markers D15Mit26 and D15Mit122 LOD 4 2 and co localizes with the gene for hyaluronan synthase Has2 Hfib2 is defined by microsatellite markers D2Mit6 and D2Mit458 LOD 4 5 and co localizes with the Hc

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file9.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    Awards POSTER 10 Tmevpg1 WHICH CODES FOR A NON CODING mRNA EXPRESSED IN THE IMMUN SYSTEM IS A CANDIDATE GENE FOR Tmevp3 locus J F Bureau Institut Pasteur 1 Vigneau S 2 Rohrlich P S 1 Brahic M 1 Unité des Virus Lents CNRS URA 1930 2 Unité Immunité Cellulaire Antivirale Tmevp3 locus controls the viral genome load during the persistent infection of mouse central nervous system CNS by Theiler s virus We have identified a candidate gene for this locus named Tmevpg1 by a positional cloning approach The murine Tmevpg1 gene and its human ortholog TMEVPG1 are expressed in the immune system and encode what appears to be a non coding RNA They are located in cluster of cytokines which includes interferon gamma Ifng and one or two homolog of interleukin 10 We now report that Tmevpg1 is expressed in uninfected splenocytes of both resistant B10 S mice and SJL J mice congenic for Tmevp3 locus of B10 S haplotype but is not detected in the case of susceptible SJL J mice A similar pattern of expression has been found in CNS infiltrating immune cells following inoculation by Theiler s virus Tmevpg1 is down regulated after in vitro stimulation

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file10.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    Genetics Furuse T Shiroishi T Koide T National Institute of Genetics NIG Tokyo University of Agriture and technology In the National Institute of genetics a variety of mouse strains has been developed from the wild mice captured in many countries of all over the world A series of wild derived strains is now called as Mishima battery In this study genetic diversity in the Mishima battery is utilized to study the genes involved in the behavioral diversity Capsaicin is the major component of red pepper which causes hot taste An experiment on knockout mice for the receptor of capsaicin VR1 showed that the sensation for both capsaicin and thermal pain mediate the same receptor In order to approach the underlying genetic mechanism for diversity of preference for red pepper we conducted a 12 hr 1 bottle fluid intake test of capsaicin solution using animals from Mishima battery Relative to baseline water intake C57BL 6J and DBA 1J consumed 10 percent while KJR and MSM ingested approximately 60 percent of the 15uM capsaicin solution The results of 1 bottle test are similar to that displayed by these strains in the hot plate test In the 1 bottle test F1 progeny of

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file11.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    1 Wakabayashi Y 2 Niwa O 1 Department of Gene Regulation Graduate School of Medical and Dental Sciences Niigata University 2 Radiation Biology Center Kyoto University Individuals inheriting the same mutation predisposing to cancer often express different phenotypes with respect to spectrum of tumors and age of onset Li Fraumeni patients with p53 gene mutations are susceptible to sarcomas and carcinoma of the breast and their outcomes range from early aggressive cancer to disease free survival This may reflect the presence of low penetrance genes in the human population as well as environmental factors that can modify the impact of p53 mutation Identification of the p53 modifiers is important for its public health implications and also gives clues to signaling pathways in cells lacking p53 the most frequently mutated gene in human cancers p53 KO mice develop tumors at a very young age and heterozygotes are extremely susceptible to radiation induced thymic lymphomas To search the modifiers we have performed genome scan for 160 lymphomas and 69 skin tumors induced by ray irradiation of p53 KO backcross mice between BALB c and MSM strains BALB c derived alleles at three loci on chromosome 19 Mp53D1 modifier of p53 deficiency at

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file12.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    Mouse Models Human Disease and Pharmacogenetics Sequence Annotation and Comparative Analysis of Genomes Attendees Sponsors Table of Contents Photographs Awards POSTER 13 The CAST Ei Strain Confers Significant Protection Against ApcMin Intestinal Polyps Independent of the Resistant Modifier of Min 1 Mom1R Locus R Koratkar Kimmel Cancer Center Jefferson Medical College 2 Pequignot E 2 Hauck WW and 1 Siracusa LD 1 Department of Microbiology and Immunology Kimmel Cancer Center 2 Biostatistics Section Jefferson Medical College Intestinal adenoma development in ApcMin mice is influenced by genetic background We generated a congenic line between the CAST and B6 inbred strains to study the effects of a resistant CAST background in the absence of a major modifier locus Modifier of Min 1 Mom1R Progeny from a CAST B6 Mom1R S x B6 ApcMin intercross were aged to 110 or 200 days and screened for intestinal polyps There was a significant decrease p 0 0001 in polyp multiplicity and size in CASTB6F1 Mom1R S ApcMin and CASTB6F1 Mom1S S ApcMin progeny compared to B6 Mom1S S ApcMin controls A complete absence of colon polyps was observed in all mice heterozygous for the CAST background These results demonstrate that the CAST strain carries dominant

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file13.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    Research Group Infection Genetics 2 Department of Microbial Pathogenesis and Vaccine ResearchDivision of Microbiology German Research Center for Biotechnology GBF Streptococcus pyogenes Group A streptococcus GAS is an important bacterial Gram positive extracellular pathogen which can cause a variety of clinical manifestations in humans By infecting different inbred strains of mice with S pyogenes we observed that resistance susceptibility to GAS infections is genetically determined BALB cJ C57BL 10J and DBA 2J mice were found to be the most resistant strains able to clear and survive the infection In contrast C3H HeN A J and CBA J mice were much more susceptible and their inability to control bacterial growth resulted in bacteremia and death In addition susceptibility to S pyogenes infection was influenced by sex with males being more susceptible than females A genetic linkage study was initiated to investigate the observed differences to S pyogenes infection We used BALB c and C3H HeN mice in a backcross mapping experiment and infected the female F2R progeny with S pyogenes These animals were monitored for survival time and bacterial blood counts post infection respectively In a genome wide screen we identified a QTL on chromosome 7 controlling resistance to S pyogenes

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file14.shtml (2016-02-17)
    Open archived version from archive

  • The 16th International Mouse Genome Conference (2002)
    M Johansson Å C M Holmdahl R 1 Lund University To dissect the interaction between susceptibility loci for Collagen Induced Arthritis CIA we have established a partial advanced intercross between congenic strains This experimental set up is accompanied by statistical analysis of gene interaction Rheumatoid arthritis RA is an autoimmune syndrome diagnosed by the American College of Rheumatology ACR criteria reflecting signs and symptoms of chronic erosive inflammation of diarthrodial joints RA is characterized by symmetrical swelling and pain of the joints stiffness and fatigue The genetic influence is of polygenic nature where the different genes most likely interact in disease susceptibility CIA in mice is a well established model for human RA and the model used in the present study We have previously identified two main susceptibility loci for CIA in a NOD Q X C57Bl 10 Q intercross Cia9 on chromosome 1 and Cia2 on chromosome 2 Since both parental strains carry the H2q haplotype the influence of the H2 complex could be disregarded The Cia9 and Cia2 loci were confirmed in a backcross experiment using the same parental strains Analysis of two locus interaction of both crosses using the QTL analysis application R qtl established a model

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file15.shtml (2016-02-17)
    Open archived version from archive



  •