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  • The 16th International Mouse Genome Conference (2002)
    D Blake JA Bult CJ Eppig JT and the Mouse Genome Informatics Staff The Jackson Laboratory The Mouse Genome Database MGD is the premier mouse data resource serving the scientific community MGD allows investigators to address complex queries for information from multiple sources in a fast and effective manner Our goal at MGD is to facilitate the progress of scientific research by providing high quality easily accessible data MGD is actively developing a robust representation of quantitative trait loci QTLs QTLs are regions of the genome with statistically significant linkage to a quantifiable trait and are mapped using crosses of inbred strains differing in measurable traits such as blood pressure or life span Each QTL region contains a gene or genes underlying the trait measured which may then be identified via candidate gene analysis or positional cloning QTL analysis is invaluable for dissection of complex diseases often the culmination of multiple interacting genetic factors QTLs are currently represented in MGD as a comprehensive summary of the mapping experiment which includes information such as the LOD score QTL range linked markers cross information mode of inheritance and potential candidate genes As the text summary is not searchable it is our goal

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file16.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    Williams R 1 University of Tennessee 2 University of North Carolina Recombinant inbred intercross RIX mapping is a new gene mapping method that extends the power of existing sets of recombinant inbred strains Here we used the CXB RIX set to map quantitative trait loci QTLs that modulate hippocampus size Our mapping panel consists of all 13 CXB parental strains and the full set of 78 non reciprocal F1 RIX hybrids The hippocampus was dissected from fixed brain and weighed immediately The average bilateral hippocampal weight of CXB strains is 27 1 2 55 mg whereas the average of RIX progeny is 28 6 1 85 mg We corrected for differences in age sex body weight and brain weight by multiple linear regression The adjusted data the weight residuals range from 10 4 to 6 0 mg in CXB strains and from 9 9 to 3 8 mg in RIX F1 mice Map Manager QTX program was used for QTL analysis Mapping in CXB parental strains detects only one weak QTL on Chr 11 with LRS of 11 8 between 24 and 33 Mb Using the large RIX panel we verified this QTL but the LRS increased to 32 3

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file17.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    JD 2 Threadgill D 4 Williams RW 1 Roswell Park Cancer Institute 2 Univ Tennessee Memphis 3 University of Alabama Birmingham 4 University of North Carolina Using high density oligonucleotide arrays Affy U74Av2 we measured expression of 12422 transcripts in forebrains of 24 lines of adult BXD mice including parents and F1 Experimental noise was reduced by pooling and replication 3 cases array and 1 to 4 arrays line Log transformed expression data average difference between PM and MM probes for each array were normalized to standard mean and SD before replicate array data were averaged Variation in transcript level was mapped by least squares regression against genotypes of 508 non redundant BXD markers using custom software written in C and Python Empirical p values for each transcript locus association were calculated by permutation tests with up to 1000000 permutations Only the most significant marker association was retained for each transcript These were ordered by p value and tested according to Benjamini and Hochberg at false discovery rate 0 2 This test identified 74 QTLs distributed across almost all chromosomes Repeating this procedure with data from individual probes about 400 000 declared 576 probe locus combinations representing 339 transcripts as

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file18.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    K Lyons PA University of Cambridge Linkage analysis of the Non Obese Diabetic mouse NOD genome has revealed the location of several non MHC genes involved in type 1 diabetes The breeding of novel congenic mouse strains has confirmed that one of these Idd9 on mouse chromosome 4 is a true locus Subsequent subcongenic mapping has shown that the original effect is due to the interaction of at least three distinct loci Idd9 1 Idd9 2 and Idd9 3 An unexpected outcome of the subcongenic analysis was that removal of the proximal region of the original congenic segment led to a significant reduction in diabetes frequency suggesting the presence of a diabetes susceptibility gene conferred by the B10 allele Genotyping a panel of mice from a cross between NOD and B10 H2g7 with the microsatellite markers D4Mit82 and D4Mit28 put the interval size at 20 6cM High resolution mapping using a panel of 18 microsatellite markers refined the interval to 15 2cM between the microsatellite markers D4Mcg25 and D4Mit144 Based on the current draft of the mouse genome sequence D4Mcg25 and D4Mit144 are located at 63 9Mb and 90 2Mb respectively on mouse Chromosome 4 Additional microsatellite markers have been

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file19.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    Human Disease and Pharmacogenetics Sequence Annotation and Comparative Analysis of Genomes Attendees Sponsors Table of Contents Photographs Awards POSTER 20 THE EFFECT OF THE GTROSA26 INSERTION ON SUSCEPTIBILITY TO MAMMARY AND INTESTINAL TUMOR DEVELOPMENT IN ApcMin Mice A Moser University of Wisconsin Kohlhepp R Hegge L 1 University of Wisconsin Approximately 5 of C57BL 6J B6 female mice heterozygous for ApcMin Min a nonsense mutation at codon 850 of the murine Apc locus develop spontaneous mammary tumors Treatment with ethylnitrosourea ENU results in about 90 of B6 Min females developing multiple mammary tumors within 65 days However B6 Min mice carrying the Gtrosa26 gene trap insertion on chromosome 6 are resistant to mammary tumor development We have found that two independently derived congenic lines of B6 ROSA26 mice carry more than 25 cM of DNA from the 129 strain flanking the insertion To map the locus affecting mammary tumor development within this region we identified and characterized several lines of mice carrying smaller sections of the congenic region Only the mice carrying a congenic interval containing the Gtrosa26 insertion are resistant to mammary tumor development The Gtrosa26 insertion also affects intestinal tumor growth but not multiplicity We have further shown

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file20.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    THE 129 MOLF CHR19 CHROMOSOME SUBSTITUTION STRAIN AND DERIVED CONGENIC STRAINS K Youngren Case Western Reserve University 2 Handel MA 3 Matin A 1 Nadeau JH 1 CWRU 2 University of Tennessee 3 University of Texas Testicular germ cell tumors TGCTs are the most common solid cancers affecting young men Although there is evidence for genetic predisposition to TGCTs the genetic control of susceptibility is poorly understood The 129S1 SvImJ mouse strain is an excellent model system for studying TGCTs This strain develops TGCTs at a frequency of 1 10 depending on the subline We previously reported a new mouse strain the 129 MOLF Chr19 Chromosome Substitution Strain CSS which develops spontaneous TGCTs at a high frequency This strain was made by replacing chromosome Chr 19 of the 129 strain with the homologous chromosome from the MOLF Ei strain 82 of males of the 129 MOLF Chr19 strain develop TGCT and 57 of these TGCTs are bilateral To characterize the genetic control of TGCT susceptibility in this CSS we created a panel of congenic strains derived from the 129 MOLF Chr19 strain We found that multiple genes alone and in combination control susceptibility to TGCTs in a highly quantitative manner

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file21.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    POSTER 22 GENOME WIDE METHYLATION SCREENING IN MOUSE GENOME AN APPLICATION TO MOUSE LIVER CANCER M T K Kimura Roswell Park Cancer Institute 2 Matuyama T 1 Held WA 2 Yoshida S 1 Nagase H 1 Roswell Park Cancer Institute 2 RIKEN Epigenetic DNA modification such as DNA methylation within CpG islands relates to various biological phenotypes such as transcriptional regulation DNA replication timing chromatin condensation and genomic imprinting However the genome wide methylation pattern has not been examined efficiently Restriction Landmark Genomic Scanning RLGS is a method for the two dimensional display of end labeled DNA restriction fragments RLGS with methylation sensitive restriction enzymes such as NotI BssHII and XmaIII reveals random screenings of DNA methylation which covers around 1 of the genome in a RLGS gel It is however difficult to identify sequences from aberrant methylated RLGS spots To facilitate systematic analysis of RLGS data we have developed a computational tool Virtual RLGS Vi RLGS which is based on mouse genomic DNA sequence information from sequence databases Vi RLGS software predicts RLGS two dimensional electrophoretogram patterns and the DNA sequences of the RLGS signal spots We have examined the sequences of real RLGS signal spots and have confirmed

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file22.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    of North Carolina 1 Kim K 1 Pappalas J 1 Bell T 1 Howard I 2 Thomas S 1 University of North Carolina 2 Claflin University The Om locus is involved for two unusual mouse phenotypes the early embryonic death known as the DDK syndrome and sperm mediated meiotic drive during female meiosis Two genetic elements a DDK maternal factor and a paternal gene are responsible for the lethal phenotype while another two the Responder and Shade are responsible for the drive The components of the lethal phenotype and the Responder map to a 400kb interval while Shade is localized in a 1Mb non overlapping distal interval Identification and validation of candidate genes for each one of the four genetic elements has been challenging because these regions are gene rich and knockout based approaches are unlikely to replicate the phenotypes because none of the alleles are predicted be represent null mutations To overcome these problems we have defined the functional alleles present in over 10 phylogeneticaly distant inbred strains These include laboratory strains of common use as well as wild derived strains from castaneus and molossinus origins Our results demonstrate that each element is a separate locus and that different

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file23.shtml (2016-02-17)
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