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  • The 16th International Mouse Genome Conference (2002)
    J 8 Zimmer A 2 Pfeffer K 3 Wolf E 1 GSF Research Center for Environment and Health Neuherberg 2 Technical University of Munich 3 Gene Center University of Munich 4 Max Delbrueck Centre Berlin 5 GSF Technical University Munich 6 Clinic of Harlaching Munich 7 Institute of Immunology GSF Research Center for Environment and Health Neuherberg 8 Polyclinic for Psychiatry University of Bonn With the completion of the human genome sequence and the prospect of a complete mouse sequence within the near future a major challange is the systematic determination of gene function in mammals The growing ENU mouse mutant resource provides a powerful entry point to gene function studies Here we give an update of one of the largest ENU mutagenesis programs in Europe the Munich ENU Mouse Mutagenesis Project Since proof of principle of a large scale ENU mutagenesis program has been demonstrated during the first phase of the project by focusing on dominant traits we put our main efforts during the last year on the recessive screen In parallel we continue to produce F1 animals to further isolate novel dominant alleles of known and new genes Currently more than 30 000 mice have been investigated for

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file68.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    69 GENE CATALOG OF MOUSE STEM CELLS AND EARLY EMBRYOS M Ko Developmental Genomics Aging Section Laboratory of Genetics National Institute on Aging NIH 1 Piao Y 1 Stagg C 1 Martin P 1 Bassey U 1 Qian Y 1 Sharov A 1 VanBuren V 1 Tanaka T 1 Jaradat S 1 Carter M 1 Kimber W 1 Aiba K 1 Hamatani T 1 Yoshikawa T 1 Matoba R 1 Sharova L 2 Kelso J 2 Hide W 1 NIA 2 South African National Bioinformatics Institute We report here our continued efforts to assemble a mouse gene catalog from early embryonic stages as well as embryonic and adult stem cells To overcome the scarcity of materials in these stages we recently developed a method to construct long insert enriched cDNA libraries from submicrogram amounts of total RNA This new method allowed us to add 80 000 new ESTs which may represent full length cDNAs to our previous collection of 80 000 ESTs These ESTs were clustered into 26 000 unique gene collections which added 11 000 new cDNA clones to the NIA Mouse 15K cDNA clone set After single colony isolation and sequence verification a new 7 4K clone set

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file69.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    M 1 Tomkies VS 1 Morris GAJ 2 Williams DJ 2 Williams G 2 Freeman T 1 University of Cambridge 2 MRC HGMP Resource Centre Congenic strain analysis has proved a powerful tool in the dissection of susceptibility to type 1 diabetes in the NOD mouse Using this approach a number of diabetes susceptibility Idd loci have been mapped to intervals small enough for systematic gene identification Combining congenic mapping and microarray based gene expression profiling will not only facilitate the identification of the genes underlying individual Idd loci but may also provide insight into the pathways they control A better understanding of the molecular events that initiate and drive disease progression is essential for the development of new drug therapies Given the autoimmune nature of type 1 diabetes we have developed a mouse immunoarray using spotted 50mer oligonucleotides as probes Genes for inclusion on the array were selected in the following manner Using the Gene Ontology hierarchies 1137 genes were identified from the Mouse Genome Database on the basis of being annotated as involved in either the immune response or transcriptional regulation In addition 1298 ESTs expressed in lymphoid derived cDNA libraries were picked from dbEST The immunoarray has

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file70.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    Larimer FW 1 Cain KT 1 Carpenter DJ 1 Easter LL 1 Foster CM 1 Gardner AW 1 Houser KJ 1 Hughes LA 1 Kerley MK 1 Lu T YS 1 Olszewski RE 1 Pinn I 1 Shaw GD 1 Shinpock SG 1 Wymore AM 1 York ML 1 2 Baker EJ 1 2 Snoddy JR 1 2 Johnson DK and 1 2 3 Rinchik EM 1 Life Sciences Division Oak Ridge National Laboratory 2 The University of Tennessee Oak Ridge National Laboratory Graduate School of Genome Science and Technology 3 Department of Biochemistry Cellular and Molecular Biology The University of Tennessee The availability of the complete DNA sequence of the mouse genome coupled with the development of high throughput methods for rapid detection of single nucleotide polymorphisms SNPs have made it practical to consider genome wide gene sequence driven approaches to mouse germline mutagenesis Such gene driven strategies allow one to perform whole genome mutagenesis and then screen for alterations in any pre selected gene s To complement embryonic stem cell based gene driven mutagenesis resources such as gene trap libraries and banks of N ethyl N nitrosourea ENU mutagenized ES cells we have been generating a cryopreserved bank of DNA tissues for RNAs and proteins and sperm from 5 000 C57BL 6JRn mice that each carry a unique load of paternally induced ENU mutations This ORNL Cryopreserved Mutant Mouse Bank CMMB is a source of induced heritable SNPs in both regulatory regions and coding sequences of virtually every gene in the genome High throughput Temperature Gradient Capillary Electrophoresis TGCE is used to identify mutations by heteroduplex analysis in pre selected genes in the CMMB DNA panel and mutant stocks are recovered by in vitro fertilization or intracytoplasmic sperm injection from the parallel bank of frozen sperm We will

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file71.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    POSTER 72 BUILDING HEARTS FROM COMPUTATIONAL ANALYSIS OF CARDIOVASCULAR TRAITS IN GENETICALLY RANDOMIZED POPULATIONS J Nadeau Genetics CWRU 1 Burrage L 2 Churchill G 1 Hill A 3 Restivo J 4 Shao H 4 Pao Y 3 Hoit B 1 Genetics CWRU 2 Jackson Laboratory 3 Medicine CWRU 4 Electrical Engineering and Computer Science CWRU A major problem in studying biological traits is understanding how genes work together to provide organismal structures and functions Conventional approaches usually involve a reductionist approach where specific functions are attributed to particular genes motifs and amino acids The equally important but harder problem involves the synthesis of information to understand functionality at higher levels We have developed a computational method called Phenotype Segregation Networks PSNs that uses assays of component traits to learn about higher level systems We used subtle naturally occurring multigenic variation of cardiovascular CV properties in the A J and C57BL 6J strains and the AXB BXA RI strains to perturb CV functions in non pathologic ways In this proof of concept study computational analysis correctly identified the known functional relations among CV properties and revealed key aspects of heart functions This PSN was then used to account for pleiotropies in

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file72.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    of Animal Health Japan 3 International Livestock Research Institute Susceptible A J mice develop higher parasitemia but less severe anemia than resistant C57BL 6 mice following Trypanosoma congolense infection Parasitemias in A J are even higher than in TNF a deficient C57BL 6 mice which are also very susceptible These observations suggest that A J mice are capable of suppressing protective responses having detrimental effects to the host such as anemia Glucocorticoids play a central role in suppression of inflammatory and immune responses Therefore glucocorticoid responses were compared between A J wild type and TNF a deficient C57BL 6 mice up to day 17 following infection Serum corticosterone was determined by radioimmunoassay The expression level of Cyp21a which is located within the major trypanosome resistance QTL Tir1 and encodes an enzyme involved in corticosterone synthesis was monitored by RT PCR in total RNA extracted from adrenal glands Corticosterone concentrations in wild type and TNF a deficient C57BL 6 remained low except for a transient slight increase at day 7 However by day 4 corticosterone increased in A J to higher levels than in C57BL 6 and thereafter gradually returned to the pre infection levels A lower expression of Cyp21a mRNA

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file73.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    LABORATORY MOUSE LN Ni Informatics The Jackson Laboratory Blake JA Bult CJ Ringwald M Kadin JA Richardson JE Eppig JT and the Mouse Genome Informatics Group The Jackson Laboratory The Mouse Genome Informatics MGI http www informatics jax org is a comprehensive integrated public information resource for mouse genetics genomics and biology It fully integrates information on over 32 000 mouse genes and experimental data from over 77 000 references MGI provides extensively curated mouse data encompassing mouse gene nomenclature mutant and strain phenotypes embryonic gene expression patterns molecular segments and sequence clusters genetic physical and comparative maps allelic variations and mammalian homology information MGI also collaboratively curates data with SWISS PROT and NCBI LocusLink and provides extensive curated links with these and other online resources such as GenBank PubMed and OMIM One of the fundamental goals of the Mouse Genome Informatics is to functionally annotate the mouse genome Towards this goal MGI is actively engaged in the development and application of controlled vocabularies developed by the Gene Ontology consortium to annotate the biology of gene products at the level of their cellular location the biological processes and their molecular function Other structured vocabularies of phenotype descriptors and anatomical terms

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file74.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    LOH in the region of human 1p32 36 has been observed in a wide variety of cancers including leukemia breast lung intestinal liver and neuroblastoma In particular a deletion of 1p32 36 is frequently observed in the blast crisis phase of chronic myelocytic leukemia This suggests that there are one or more tumor suppressor genes that suppress progression to the malignancy in the region of 1p32 36 Using the Cre loxP chromosome engineering strategy we have generated a series of ES cell lines and mice with large deletions and inversions on the distal region of mouse chromosome 4 In sum this deletion series encompass a 40cM region of mouse chromosome 4 and the size of each deletion is 1 5cM The deletion events were confirmed by fluorescence in situ hybridization Deletion strains exhibiting an increased leukemia susceptibility will be analyzed in a combination with mutated oncogenes and tumor suppressor genes such as Ras p53 and bcr abl which is accelerate tumorigenesis In addition to large deletions two mouse strains that carry large inversions on the distal region of mouse chromosome 4 were generated The inversion intervals are 16 and 22 cM in size in sum they cover approximately half of

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file75.shtml (2016-02-17)
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