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  • The 16th International Mouse Genome Conference (2002)
    CONTENT OF MOUSE CHROMOSOME 17 D Bergstrom The Jackson Laboratory 1 Munroe R 1 Bergstrom R 2 Browning V 3 You Y 1 Schimenti J 1 The Jackson Laboratory 2 Univ of Wisconsin Madison 3 Oak Ridge National Laboratory Chromosome deletion complexes in model organisms serve as valuable genetic tools for the functional and physical annotation of complex genomes Among their many roles deletions can serve as mapping tools for simple or quantitative trait loci QTLs genetic reagents for regional mutagenesis experiments or in the case of mice provide models of human contiguous gene deletion syndromes They are also uniquely suited for identifying regions of the genome containing haploinsufficient or imprinted loci Using the technique of ES cell irradiation we have created and characterized a comprehensive set of deletions at the proximal end of mouse Chromosome 17 that in total cover much of the mouse t complex The deletions are arranged in 5 overlapping complexes that collectively span 25 30 cM Furthermore we integrate molecular data from each of the deletion complexes with physical data from the public mouse genome BAC contig and Celera s mouse genome sequence using the deletions as mapping reagents to resolve some discrepancies We have

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file84.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    Jones J 1 Jones P 1 Oxspring R 1 Seymour M 2 Johnson R 1 Informatics Group MRC Harwell 2 Mary Lyon Centre MRC Harwell The Mary Lyon Centre MLC is a state of the art central facility for mouse genetic studies being constructed on the MRC Harwell campus To support the MLC a team of software developers within the MRC Harwell Informatics Group is developing a data capture system to incorporate all aspects of animal facility research and management The system AnonyMus consists of a relational database management system schema Sybase and a web enabled user front end implemented using Java technologies servlets and Java Server Pages The software is being designed to ensure stability security efficiency accuracy and ease of use to provide an online environment where animal husbandry experimental and facility management data are accessible alongside licensing data to ensure compliance with UK Home Office regulations To enable successful design development and deployment each subject area is split into modules and these are assigned to individual programmers Programmers are able to gain a complete understanding of the scientific principles of the areas assigned to them ensuring development of software to meet specific user requirements To aid development

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file85.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    T 1 Kobayashi K 1 Kaneda H 1 Ishijima J 2 Nakai Y 2 Gondo Y 1 Wakana S 1 Noda T and 1 Shiroishi T 1 Mouse Functional Genomics Research Group RIKEN GSC2 Population and Quantitative Genomics Team RIKEN GSC Mouse ENU mutagenesis program in RIKEN GSC directs a large scale production of mutant mice by comprehensive phenotype screening The program involves the screenings for early and late onset phenotypes In the former screening about 100 mice are every week subjected to behavioral test based on the modified SHIRPA protocol blood test clinical biochemical and hemogram In the latter screening about 60 mice aged up to 18 months are subjected to package of behavioral tests that include Openfield test for assaying anxiety and Passive avoidance test for learning and memory At the end of the late onset screening all aged mice are dissected for observe the tumor incidence Sperm of all G1 animals are archived by cryopreservation for the late onset screening and gene driven analyses Phenodeviants are examined inheritance of its phenotypes by the offspring Mutants are deposited to RIKEN BRC for conservation of mutant resources All the experiments in this program are supported by the central database

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file86.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    M 1 Reid T 2 To C 2 Tsao N 1 Yu M 2 Kassam N 1 Osborne L 2 Rossant J 1 Stanford WL and the CMHD 1 University of Toronto 2 Samuel Lunenfeld Research Institute We are performing gene trap based expression and genotypic screens to generate new mouse mutations that will help delineate the molecular controls of specific developmental programs Random insertion of the gene trap vectors containing a splice acceptor immediately upstream of a promoterless reporter into the mouse embryonic stem ES cell genome provides a method to generate loss of function mutations and report expression for many and given new vectors potentially all mouse genes A series of polyA trap vectors have been developed to attempt to optimize insertions into all classes of genes irrespective of their expression in undifferentiated ES cells The gene trap insertions are screened using multiplexed in vitro differentiation and induction assays An expression profile for each clone is being generated being generated and will be available via an online searchable database by the time of the International Mammalian Genome Conference http www cmhd ca sub genetrap htm RACE PCR is being performed to complement expression profiles with sequence tags which

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file87.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    Medicine 2 Wang X 3 Bradley A 1 2 Baylor College of Medicine 3 The Wellcome Trust Sanger Institute We have devised a method to isolate gene trap mutations on a chromosome of interest in mouse embryonic stem ES cells using Cre loxP technology Specifically we used ES cells with a neo selection cassette a loxP site and the 5 part of the Hprt mini gene targeted to the Hsd17b1 locus on the distal part of mouse chromosome 11 We infected these cells with a gene trapping retrovirus that contains a 3 gene trap construct a loxP site and the 3 part of the Hprt mini gene If a proviral insertion and gene trap occurred on chromosome 11 we isolated it by selecting for a Cre mediated inversion between the targeted locus and the gene trap locus The inversion event reconstitutes a functional Hprt gene and is selected in HAT containing media By selecting for inversions we isolated gene traps proximally and distally to the targeted locus on chromosome 11 To date we have isolated 21 gene traps on chromosome 11 We were able to select gene traps within a 117 Mb region however most 60 of the gene traps

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file88.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    de Angelis M 1 CNR 2 CNRS 3 MRC 4 Karolinska Institutet 5 Fondação C Gulbenkian 6 EBI 7 GSF In order to make the transfer of biological information more effective appropriate databases are under construction and will be further developed in the framework of the European Mouse Mutant Archive EMMA and in collaboration with existing databases An essential role of the EMMA project is to foster the virtual coupling of the EMMA stock centres and related information systems through the establishment and maintenance of a dedicated resource database EMMA RDB This will be ultimately implemented as a fully integrated web based database It will connect the different partners to one virtual centre and serve as an interface to the scientific community EMMA currently develops towards the central European repository for cryopreservation and distribution of mouse mutant strains EMMA strains are available to academic institutions from all around the world Stocks may also be available to industrial commercial organisations although their use may be restricted in accordance with specific Material Transfer Agreements held by the original owners producers of the requested strain A focus is the establishment of a worldwide network of repositories for all generated mutant lines while EMMA

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file89.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    STERILITY C Poirier Baylor College of Medecine 2 Harrison W 2 Overbeek P 1 Bishop CE 1 Dpt of Obstetrics and Gynecology 2 Dpt of Molecular and Cell Biology Baylor College of Medecine In order to create a panel of insertional mutations in the mouse a transgene was inserted by microinjection into the fertilized eggs of albino FVB N mice This 5 5kb transgene was carrying a tyrosinase minigene TyBS the mouse tyrosinase cDNA under the control of its own promoter Transgenic founders were identified by their dark coat color and mated to FVB N For each independent transgenic line the carrier offspring TyBS were intercrossed and the coat color used as an indicator of segregation for the transgene to identify heterozygous TyBS dark mice and homozygous carriers TyBS TyBS darker mice For the line OVE 979 the 3 genotypes of mice non carriers TyBS and TyBS TyBS were born in normal Mendelian frequency and no obvious phenotype except coat color segregated with the transgene For each line 3 mating pairs of homozygous carriers TyBS TyBS were set up For OVE 979 no pups were born after 3 months of breeding suggesting that in this line mice homozygous for the

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file90.shtml (2016-02-17)
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  • The 16th International Mouse Genome Conference (2002)
    Sequence Annotation and Comparative Analysis of Genomes Attendees Sponsors Table of Contents Photographs Awards POSTER 91 USE OF CDNA ARRAY HYBRIDIZATION AND EXPRESSION ANALYSES IN GENE TESTING FOR ENU INDUCED MUTATIONS IN MICE P Charmley Celltech R D Inc Bennington A Snell A Appleby M Strobel S Celltech R D Inc As part of a mutant gene testing approach in our ENU mouse mutagenesis program we are using oligo based array hybridization technology to aid in finding the ENU induced phenotype causing mutation in our mutant mice Our application of this technology has involved the duplicate spotting of 60 mer oligos from every known or predicted gene in the genetically mapped interval that contains the phenotype causing mutation We have used real time PCR to assess the robustness of the array technology through side by side comparisons of the expression measurements By our first applying this array hybridization approach to an interval with a known ENU mutation we are gaining confidence in the strengths and limits of this technology We are using the expression results to prioritize which genes are sequenced for the mutation as well as the possibility for identifying mutants that alter the gene expression levels We hope

    Original URL path: http://www.imgs.org/Archive/abstracts/2002abstracts/file91.shtml (2016-02-17)
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