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  • From sampling methodology to Metabarcoding, a new way to use environmental DNA (eDNA) as a tool for fish biodiversity assessment in stream waters - metabarcoding.org
    an alternative at catch release methodologies and other sampling methods Furthermore the synergy between eDNA and Metabarcoding is even more attractive for its sensitivity and its potential breakthrough in Ecology Two institutions and a start up company decided to join their knowledges Irstea LECA and Spygen to test the efficiency of this new approach fish biodiversity assessment for Irstea molecular analysis NGS knowledge and metabarcoding analysis for Spygen and the LECA The aim of this collaboration is to create a new sampling methodology of eDNA in stream waters to improve the eDNA analysis for the fish biodiversity and to interpret the recovered signal through Metabarcoding and Models Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding sparking conversations between the attendees The end of the first day saw flash talks from all participants of the event creating an icebreaker

    Original URL path: http://metabarcoding.org/spip.php?article40 (2016-04-26)
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  • DNA metabarcoding in biodiversity research - metabarcoding.org
    Is this error random or systematic If it is systematic e g species 1 systematically has a 50 lower yield than species 2 a correction factor can be applied to the final result DNA amplification bias for problematic taxa will be further estimated using known prior DNA concentrations from tissue samples and qPCR Analysis bias Here we will compare metabarcoding results from three independent laboratories to estimate the amount of variance purely caused by laboratory bound differences Two metabarcoding approaches will be used The first will be based entirely on environmental DNA which will be used for fish and amphibian communities This allows one to test for the severity of all above sources of error The second will be based on unsorted community samples of macro invertebrates and zoobenthos oligochaetes molluscs arthropods and on unsorted zooplankton samples These will be homogenized prior to subsampling and DNA extraction There we will only be able to detect amplification and analysis bias Because many zoobenthos and zooplankton species are difficult to identify and contain many cryptic species many taxa are only identified at family level or higher levels e g calanoid copepods versus cyclopoid copepods a metabarcoding approach is expected to provide greater taxonomic resolution for ecological studies and greater sensitivity to detect uncommon species Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw

    Original URL path: http://metabarcoding.org/spip.php?article39 (2016-04-26)
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  • metabarcoding.org
    and abundance of benthic communities through metabarcoding of environmental DNA eDNA To be considered for this position please send your CV and brief email text explaining your suitability to Read more of Developing metabarcoding tools for environmental DNA based biomonitoring in aquatic ecosystems Latest articles Cat hair silent witness of crime scene 1 March 2012 by BAKSAY Sandra The use of non human DNA found on crime scenes can improve the effectiveness of forensic Read more of Cat hair silent witness of crime scene Benthic vs pelagic contribution in the diet of marine benthic primary consumers a multi tool approach 1 March 2012 by MARCHAIS Violette My PhD aims at determining the precise diet of three marine benthic primary consumers abalone Read more of Benthic vs pelagic contribution in the diet of marine benthic primary consumers a multi tool approach DNA barcoding at the Museum Koenig 1 March 2012 by ASTRIN Jonas In the presentation given for the fellow Metabarcoding Spring School participants in Pinsot Read more of DNA barcoding at the Museum Koenig Phylogeny of anaerobic gut fungi 29 February 2012 by MRAZEK Jakub The talk will deal with characterization of anaerobic fungi focusing on recent attempts to Read more of Phylogeny of anaerobic gut fungi Variability of vole food preferences new insights using DNA metabarcoding 29 February 2012 by SOININEN Eeva The general framework of generalist herbivore food selection theory has been developed using Read more of Variability of vole food preferences new insights using DNA metabarcoding 0 20 25 30 35 40 45 50 55 60 65 Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble

    Original URL path: http://metabarcoding.org/?debut_articles=50 (2016-04-26)
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  • metabarcoding.org
    bg br brezhoneg bs bosanski ca català co corsu cpf Kréol réyoné cpf hat Kréyòl Péyi Dayiti cs čeština da dansk de Deutsch en English eo Esperanto es Español eu euskara fa fon fongbè fr français fr fem français féminin gl galego he hr hrvatski hu magyar id Indonesia it italiano it fem italiana ja km lb Lëtzebuergesch my Burmese nl Nederlands oc auv òc auvernhat oc gsc òc gascon oc lms òc lemosin oc lnc òc lengadocian oc ni òc niçard oc ni la òc niçard larg oc ni mis òc nissart mistralenc oc prv òc provençau oc va òc vivaroaupenc pl polski pt Português pt br Português do Brasil ro român ru sk slovenčina sv svenska tr Türkçe vi Ti ng Vi t zh Personal identifiers Login or e mail address Password password forgotten Remember my ID for a few days Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced

    Original URL path: http://metabarcoding.org/spip.php?page=login&url=.%2F%3Fdebut_articles%3D30 (2016-04-26)
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  • Studying microbial diversity - metabarcoding.org
    posed by working with microbes in nature forming large highly diverse but elusive populations However this methodological revolution must consider conceptual and methodological matters to correctly interpret microbial diversity and its patterns During this talk I will present advances gaps and caveats of these recent approaches for studying microbial diversity I ll discuss potentials and limitations of the available methodologies from sampling to sequence analysis and suggest alternative ways to incorporate results in a conceptual and methodological framework Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding

    Original URL path: http://metabarcoding.org/spip.php?article36 (2016-04-26)
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  • ZINGER Lucie - metabarcoding.org
    of your message Required Please leave this field empty Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding sparking conversations between the attendees The end of the first day saw flash talks from all participants of the event creating

    Original URL path: http://metabarcoding.org/spip.php?auteur38 (2016-04-26)
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  • Identification of mosquito blood meals using cytochrome c oxidase and cytochrome b PCR - metabarcoding.org
    from the bloodmeals of mosquitoes Diptera Culicidae The protocols used one pair of COI primers and one pair of Cyt b primers to amplify short segments of DNA Resultant sequences were then compared with sequences in GenBank using the BLAST function for putative host identification Vertebrate DNA was amplified from 121 of our sample of 300 wild caught blood fed mosquitoes from Zika forest Uganda and GenBank BLAST searches putatively identified 98 of the amplified sequences including amphibians reptiles mammalian and avian species Criteria and caveats for putative identification of blood meals are discussed Attached documents kaddumukasa pinsot 2012 pdf PDF 4 7 Mb Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding sparking conversations between the attendees The end of the first day saw flash talks from all participants of the event creating an icebreaker and showing the

    Original URL path: http://metabarcoding.org/spip.php?article35 (2016-04-26)
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  • From Swaziland to the UK - next generation sequencing on bat faeces - metabarcoding.org
    pellets from two free tailed bat species from Swaziland little free tailed bat Chaerephon pumilus and Angolan free tailed bat Mops condylurus were carried out using the Roche FLX sequencing platform to deep sequence mini barcode cytochrome coxidase I COI fragments 157 bp In order to further increase the power and economy of the method uniquely tagged primers were used to amplify DNA from each pellet thereby enabling the parallel sequencing of prey sequences from all faecal samples whilst tracking the origin of prey sequences Although the analyses were challenged by the paucity of southern African insect COI sequences in the GenBank and BOLD databases similarity to existing collections allowed the preliminary identification of 25 families from six orders of insects within the diet of C pumilus and 24 families from seven orders within the diet of M condylurus Future studies will be on greater horseshoe bat Rhinolophus ferrumequinum and lesser horseshoe bat Rhinolophus hipposideros in the UK where I will investigate the bats diet and whether next generation sequencing can be used to assess nocturnal insect diversity Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding

    Original URL path: http://metabarcoding.org/spip.php?article28 (2016-04-26)
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