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  • Enhancing freshwater ecosystem biomonitoring: Defining and testing a next generation sequencing biodiversity identification framework. - metabarcoding.org
    techniques we have the ability to overcome these difficulties Using cytochrome oxidase subunit I COI barcodes we can accurately identify specimens and resolve taxonomic issues while with Next Generation Sequencing NGS we can achieve high taxonomic resolution for DNA extracted directly from bulk samples environmental DNA In the context of this work we will populate a reference library for about 200 freshwater macroinvertebrate indicator species in the UK using barcode analysis of the COI mtDNA gene along with classical taxonomic identification Using this library and the sequences already available in databases BOLD we will be able to test the application of large community analysis of benthic macroinvertebrates from bulk environmental samples through 454 Roche pyrosequencing Furthermore we will apply a barcoding analysis system for the identification of Chironomid Pupal Exuviae CPE Technique a method which is also extensively used by the EA for the monitoring of lake ecosystems This work will set a framework for the direct application of molecular methods for the assessment and monitoring of river and lake ecosystem health Attached documents bista pinsot 2012 pdf PDF 2 4 Mb Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with

    Original URL path: http://metabarcoding.org/spip.php?article29 (2016-04-26)
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  • metabarcoding.org
    practicals All the lectures and the practicals will be taught in English The number of participants in the lecture portion is not limited but Read more of The sixth DNA metabarcoding Spring School at Kunming Latest articles Assigning and interpreting on aDNA data from a past Greenland ecosystem 29 February 2012 by SCHMIDT Astrid Ancient DNA from basal ice of a Greenland ice core was amplified on a high throughput Read more of Assigning and interpreting on aDNA data from a past Greenland ecosystem Diet analysis of the Mediterranean shag Phalacrocorax aristotelis desmarestii using conventional techniques and next generation sequencing 29 February 2012 by KASAPIDIS Panagiotis The Mediterranean shag is an avian subspecies endemic of the Mediterranean Its global Read more of Diet analysis of the Mediterranean shag Phalacrocorax aristotelis desmarestii using conventional techniques and next generation sequencing Molecular and morphological approaches to biodiversity assessment of soft bottom assemblages 27 February 2012 by GUARDIOLA Magdalena We attempt to estimate benthic biodiversity in Mediterranean marine soft bottoms macro and Read more of Molecular and morphological approaches to biodiversity assessment of soft bottom assemblages Standard and molecular methods used toward a better understanding of centipedes 27 February 2012 by ION Mihaela Centipedes are an important class of the predatory arthropod fauna Although the Romanian Read more of Standard and molecular methods used toward a better understanding of centipedes Human Identification through Analysis of the Salivary Microbiome 27 February 2012 by LEAKE Sarah My PhD focuses on investigating the potential for bacteria found in the salivary microbiome to Read more of Human Identification through Analysis of the Salivary Microbiome 0 20 25 30 35 40 45 50 55 60 65 Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land

    Original URL path: http://metabarcoding.org/?debut_articles=55 (2016-04-26)
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  • metabarcoding.org
    bg br brezhoneg bs bosanski ca català co corsu cpf Kréol réyoné cpf hat Kréyòl Péyi Dayiti cs čeština da dansk de Deutsch en English eo Esperanto es Español eu euskara fa fon fongbè fr français fr fem français féminin gl galego he hr hrvatski hu magyar id Indonesia it italiano it fem italiana ja km lb Lëtzebuergesch my Burmese nl Nederlands oc auv òc auvernhat oc gsc òc gascon oc lms òc lemosin oc lnc òc lengadocian oc ni òc niçard oc ni la òc niçard larg oc ni mis òc nissart mistralenc oc prv òc provençau oc va òc vivaroaupenc pl polski pt Português pt br Português do Brasil ro român ru sk slovenčina sv svenska tr Türkçe vi Ti ng Vi t zh Personal identifiers Login or e mail address Password password forgotten Remember my ID for a few days Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced

    Original URL path: http://metabarcoding.org/spip.php?page=login&url=.%2F%3Fdebut_articles%3D35 (2016-04-26)
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  • Trophic transfer, bioaccumulation and effects of contaminants in terrestrial food webs - metabarcoding.org
    development of molecular methods is promising to evaluate diet diversity with higher precision and more quickly On a partially remediated former gold mine in southern France Salsigne we studied arsenic transfer in a food web involving small mammals via a molecular assessment of both herbivorous and insectivorous diet We especially measured the exposition of animals to arsenic contamination of food and the distribution of arsenic in organs at 4 sessions of capture at 2 seasons spring and autumn and on 4 levels of remediation In parallel we began to analyze the diet of the 4 species wood mouse Algerian mouse common vole and greater white toothed shrew using the new metabarcoding approach on both stomach content and faeces to detect animal preys and plant food items We used the trnL method to analyse the plant diet of 10 small mammals First results are very promising Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the

    Original URL path: http://metabarcoding.org/spip.php?article33 (2016-04-26)
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  • Construction of curated taxonomic reference libraries for DNA metabarcoding - metabarcoding.org
    collections but we also carry out new collecting in the field and use other museum collections worldwide I will present our newly developed DNAbank collection management system COREMA Emphasis will be put on problems with quality assessment error detection and taxonomic completeness of the libraries Given the expected move of metabarcoding towards approaches using capture probes or sequencing of whole organellar genomes we must realize the importance of coupling our reference libraries to well curated collections of specimens and DNA extracts which can be re used to construct new libraries fitting the new approaches The best solution will be to utilize the vast specimen and DNA extract collections being assembled by standardized barcoding initiatives such as iBOL Attached documents 2012 03 05 for web site brochmann pinsot metabarc course compatibility mode PDF 6 2 Mb Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations

    Original URL path: http://metabarcoding.org/spip.php?article34 (2016-04-26)
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  • Taphonomy of Vertebrate Dirt DNA - metabarcoding.org
    DNA originating from free ranging vertebrate species The measured Vertebrate species richness inferred from dirt DNA in surface soil was improved by increasing the sample density and spatial scale rather than increasing the amount of soil processed from individual soil samples Intriguingly the relative proportions of mammal dirt DNA haplotypes identified in the soil surface more closely reflects the total biomass of the species on site rather than the individual populations census size However animal behavior was observed to bias biodiversity estimates at the sampled scale and one species which had recently been introduced was not detected Additionally the use of marker DNA molecules in high throughput sequencing experiments allows between sample comparisons of relative haplotype frequencies This approach was used to explore different sediment types for their susceptibility to DNA leaching and general guidelines are defined as to when leaching is likely to affect diversity estimates from ancient sediments A better understanding of this process leading to the incorrect temporal association of results is important for the analysis of sediment ancient DNA sedaDNA obtained from ancient environmental samples Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of

    Original URL path: http://metabarcoding.org/spip.php?article25 (2016-04-26)
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  • Habitat use and food preferences of a large herbivore: the moose, Alces alces in Poland revealed by radio-tracking and molecular diet analysis - metabarcoding.org
    number od moose in NE Poland is relatively high this raises conflicts between the species and timber production In our project we aim to assess the food plasticilty and food preferences of the species The project is divided into two parts molecular diet analysis of moose during winter throughout Poland and seasonal diet changes of the moose at the Biebrza river valley NE Poland We radio collared 20 moose and we started to collect stools every month for two years in order to asses changes in the food composition to find out which plant species is the most preferrred one and when Preliminary results showed that at least several plant species are eaten by moose as amplified fragments of trnL intron differed in size after PCR reaction using g and h primers Metabarcoding approach gives promise to accurately reveal food preferences of the moose and this will allow some recommendation for forest managment how to keep the species without challenging timber production eg Pinus trees Some ecological aspects will also be analyzed for example the seasonal moose migrations from wetelands to forests Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with

    Original URL path: http://metabarcoding.org/spip.php?article30 (2016-04-26)
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  • RATKIEWICZ Miroslaw - metabarcoding.org
    Send a message Your email address Required Subject Required Text of your message Required Please leave this field empty Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding sparking conversations between the attendees The end of the first day

    Original URL path: http://metabarcoding.org/spip.php?auteur33 (2016-04-26)
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