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  • Environmental DNA - metabarcoding.org
    water or soil without isolating any specific type of organism beforehand DNA extracted from faeces collected in the field can also be considered as environmental DNA Usually eDNA is a complex mixture of potentially degraded DNA According to the extraction protocol either intracellular extracellular DNA or both can be obtained Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills

    Original URL path: http://www.metabarcoding.org/spip.php?article7 (2016-04-26)
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  • DNA metabarcoding - metabarcoding.org
    Wednesday 2 November 2011 Download as PDF Tweet me DNA metabarcoding TABERLET Pierre High throughput DNA based identification of multiple species from environmental DNA eDNA Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding sparking conversations between the attendees

    Original URL path: http://www.metabarcoding.org/spip.php?article6 (2016-04-26)
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  • TABERLET Pierre - metabarcoding.org
    in French Alps 5 9 March 2012 Environmental DNA Molecular Ecology special issue on Environmental DNA DNA metabarcoding Send a message Your email address Required Subject Required Text of your message Required Please leave this field empty Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials

    Original URL path: http://www.metabarcoding.org/spip.php?auteur2&debut_auteurs=20 (2016-04-26)
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  • metabarcoding.org
    bg br brezhoneg bs bosanski ca català co corsu cpf Kréol réyoné cpf hat Kréyòl Péyi Dayiti cs čeština da dansk de Deutsch en English eo Esperanto es Español eu euskara fa fon fongbè fr français fr fem français féminin gl galego he hr hrvatski hu magyar id Indonesia it italiano it fem italiana ja km lb Lëtzebuergesch my Burmese nl Nederlands oc auv òc auvernhat oc gsc òc gascon oc lms òc lemosin oc lnc òc lengadocian oc ni òc niçard oc ni la òc niçard larg oc ni mis òc nissart mistralenc oc prv òc provençau oc va òc vivaroaupenc pl polski pt Português pt br Português do Brasil ro român ru sk slovenčina sv svenska tr Türkçe vi Ti ng Vi t zh Personal identifiers Login or e mail address Password password forgotten Remember my ID for a few days Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced

    Original URL path: http://www.metabarcoding.org/spip.php?page=login&url=spip.php%3Fauteur2 (2016-04-26)
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  • Day 2 : Primer design - metabarcoding.org
    Tweet me Day 2 Primer design BOYER Frédéric Eric Coissac The second practical propose to design primers amplifying a metabarcode marker specific of vascular plant Attached documents PDF design of markers PDF 4 1 Mb Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations

    Original URL path: http://www.metabarcoding.org/spip.php?article63 (2016-04-26)
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  • metabarcoding.org
    bg br brezhoneg bs bosanski ca català co corsu cpf Kréol réyoné cpf hat Kréyòl Péyi Dayiti cs čeština da dansk de Deutsch en English eo Esperanto es Español eu euskara fa fon fongbè fr français fr fem français féminin gl galego he hr hrvatski hu magyar id Indonesia it italiano it fem italiana ja km lb Lëtzebuergesch my Burmese nl Nederlands oc auv òc auvernhat oc gsc òc gascon oc lms òc lemosin oc lnc òc lengadocian oc ni òc niçard oc ni la òc niçard larg oc ni mis òc nissart mistralenc oc prv òc provençau oc va òc vivaroaupenc pl polski pt Português pt br Português do Brasil ro român ru sk slovenčina sv svenska tr Türkçe vi Ti ng Vi t zh Personal identifiers Login or e mail address Password password forgotten Remember my ID for a few days Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced

    Original URL path: http://www.metabarcoding.org/spip.php?page=login&url=.%2F%3Fdebut_articles%3D5 (2016-04-26)
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  • eDNA monitoring of aquatic animals based on water samples - metabarcoding.org
    a diversity of rare animals can be efficiently detected and possibly even quantified based on DNA We validated the findings in a controlled mesocosm experiment which also indicated that detectable DNA traces are near contemporary with species presence To test the eDNA approach at the very extreme we applied metabarcoding to pond water from well known localities and found that entire faunas of amphibians and fish can be accounted for by DNA in very small water samples We repeated this metabarcoding approach in a marine setting yielding similar promising results for fish and indicating a relatively high performance comparative to a range of conventional monitoring methods Overall our findings underpin the ubiquitous nature of DNA traces in the environment and support a bright future for eDNA applications in the monitoring of aquatic animal populations Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations in

    Original URL path: http://www.metabarcoding.org/spip.php?article61 (2016-04-26)
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  • Comparison of conventional otolith analysis and DNA metabarcoding approach to assess the diet of the Mediterranean Shag from pellets - metabarcoding.org
    the EU LIFE project ConShagAudMIBAGR a diet analysis was carried out using both conventional prey identification and next generation sequencing Regurgitated pellets containing undigested prey remains were collected from different colonies of the species in the Aegean and the Ionian Sea First conventional prey analysis was carried out on the pellets by identifying morphologically the indigestible part remains mainly fish otoliths Then the same pellets were subjected to amplicon sequencing using the 454 GS FLX platform By analyzing the same samples both by conventional and molecular techniques we were able to check whether molecular approaches perform better than conventional ones for prey identification at the species level and if they can give reliable semi quantitative estimations The molecular analysis produced results that were generally concordant with the otolith analysis However there were some discrepancies a molecular analysis gave systematically lower or higher relative frequency for certain prey species b there were prey species found in the otolith analysis but not in the DNA analysis and vice versa mainly in low abundance prey items c DNA analysis revealed few cases of species that had been misidentified in the otolith analysis and improved the recognition of prey items to the species level when this was not possible with the conventional analysis e g in some Gobidae and Sparidae taxa The discrepancies between the two methods may be attributed to the following reasons which remain to be evaluated a DNA results are proportional to the prey biomass while otoliths are proportional to the number of prey individuals b PCR biases so that some species may amplify better than others and be overrepresented in the number of sequences produced c misidentification in the otolith analysis or inability to record a species because the otoliths are worn or missing d false hit in the GenBank if the sequence of the species under consideration is missing Nevertheless this study shows that DNA analysis seems to be reliable for the diet analysis of seabirds and can be routinely used since the recent advances in sequencing technologies next generation sequencing render it a cost effective method Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and

    Original URL path: http://www.metabarcoding.org/spip.php?article48 (2016-04-26)
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