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  • Impact of metal and spray pollutions on the biodiversity of Astragalus tragacantha associated root-symbionts - metabarcoding.org
    and very rare plant species like Astragalus tragacantha Fabaceae that interface pollution from abandoned industrial sites and or polluted sea sprays from urban effluents Knowing that contaminated sites are generally poor in nutrients and contain a highly altered soil structure microbial root symbionts like mycorrhizal fungi and nodulating bacteria are suspected to play an important role in vegetation establishment But very little is known about the ecology of this endangered plant species In this context the aim of our work will be to decipher the symbiotic microbial communities that are associated to A tragacantha roots according to a gradient of pollution that the plant naturally encounters For this purpose NGS technologies Illumina will be used Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding sparking conversations between the attendees The end of the first day saw flash talks from

    Original URL path: http://www.metabarcoding.org/spip.php?article60 (2016-04-26)
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  • Host preference of fungal communities in a tropical forest: evidences obtained with 454 pyrosequencing of fungal ITS - metabarcoding.org
    in soils and litters are partly dependent of the specie of the tree above We selected two experimental sites to address this question a long term plantation of tropical trees and a closed plot of natural forest After extraction of total DNA from soil and litter samples the fungal ITS region of DNA has been amplified and sequenced by 454 pyrosequencing After filtering 300 000 sequences constituted the dataset and were used to calculate rarefaction curves and various diversity indices and to determine the effect of tree species on the composition of fungal communities both in plantation and in natural forest Results showed a significant spatial heterogeneity and nonrandom associations with the different tree species but with few variations of richness or evenness of fungal species in the plantation In natural forest the tree specie above modulated in a less proportion the structure of fungal communities Taken together these results suggest that host preference could partly explained the structuration of fungal communities inhabiting soils and litters in tropical forests Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in

    Original URL path: http://www.metabarcoding.org/spip.php?article59 (2016-04-26)
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  • metabarcoding.org
    bg br brezhoneg bs bosanski ca català co corsu cpf Kréol réyoné cpf hat Kréyòl Péyi Dayiti cs čeština da dansk de Deutsch en English eo Esperanto es Español eu euskara fa fon fongbè fr français fr fem français féminin gl galego he hr hrvatski hu magyar id Indonesia it italiano it fem italiana ja km lb Lëtzebuergesch my Burmese nl Nederlands oc auv òc auvernhat oc gsc òc gascon oc lms òc lemosin oc lnc òc lengadocian oc ni òc niçard oc ni la òc niçard larg oc ni mis òc nissart mistralenc oc prv òc provençau oc va òc vivaroaupenc pl polski pt Português pt br Português do Brasil ro român ru sk slovenčina sv svenska tr Türkçe vi Ti ng Vi t zh Personal identifiers Login or e mail address Password password forgotten Remember my ID for a few days Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced

    Original URL path: http://www.metabarcoding.org/spip.php?page=login&url=.%2F%3Fdebut_articles%3D10 (2016-04-26)
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  • Assessing the diversity and dynamics of microbial communities within the gut - metabarcoding.org
    early microbiological markers for the development of disease related conditions Moreover we investigate the effect of feed additives such as probiotics and prebiotics on the gut microbiota to manage the microbial communities for health purpose in humans and to improve performance in farm animals Early molecular approaches based on Sanger sequencing to characterize bacterial communities present in gut or fecal samples have long been used but they are highly labour intensive and often have poor resolution We are now interested in exploiting the potential of more accurate and powerful techniques relying on the recent development of next generation sequencing NGS such as metabarcoding to monitor intestinal population changes upon nutritional or therapeutic interventions Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding sparking conversations between the attendees The end of the first day saw flash talks from all participants

    Original URL path: http://www.metabarcoding.org/spip.php?article58 (2016-04-26)
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  • Gastrointestinal microbiota of wild and inbred individuals in two House Mouse subspecies assessed using high throughput parallel pyrosequencing - metabarcoding.org
    wild derived inbred strains using 454 pyrosequencing Sequenced individuals were selected according to balanced 2x2 experimental design their origin into wild 14 vs inbred 16 and M m musculus 15 vs M m domesticus 15 We compared alpha diversity i e number of operational taxonomic units beta diversity i e interindividual variability and microbiota composition across the four groups We found no difference between musculus and domesticus subspecies indicating that gut microbiota does not play role in the speciation process Both inbred and wild population showed the same level of microbial diversity We found strong differentiation of wild and inbred population based on the microbiota composition Laboratory mouse represents the most abundant model to study the effect of gut microbiota on host metabolism immunity and neurology We suggested that the existence of distinct microbiota in laboratory kept mice which differs in composition from the natural wild microbiota should be further considered in the biomedical research Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to

    Original URL path: http://www.metabarcoding.org/spip.php?article57 (2016-04-26)
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  • The widely used small subunit 18S rDNA molecule greatly underestimates true diversity in biodiversity surveys of the meiofauna - metabarcoding.org
    that 18S reduced diversity estimates by a factor of 0 4 relative to morphospecies whereas COI increased diversity estimates by a factor of 7 6 Moreover estimates of species richness using COI were robust among three of four commonly used delimitation metrics whereas estimates using 18S varied widely with the different metrics We show that meiofaunal diversity has been greatly underestimated by 18S eDNA surveys and that the use of COI provides a better estimate of diversity The suitability of COI is supported by cross mating experiments in the literature and evolutionary analyses of discreteness in patterns of genetic variation Furthermore its splitting of morphospecies is expected from documented levels of cryptic taxa in exemplar meiofauna We recommend against using 18S as a marker for biodiversity surveys and suggest that use of COI for eDNA surveys could provide more accurate estimates of species richness in the future Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality

    Original URL path: http://www.metabarcoding.org/spip.php?article56 (2016-04-26)
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  • “Next generation biodiversity”: Combination of molecular and morphological approaches to estimate biodiversity in lagoonal sediments - metabarcoding.org
    identification of multiple species from the sediment samples This project involves the combination of both the aforementioned techniques in the estimation of benthic biodiversity of five different lagoons The area under study is Amvrakikos Gulf Ionian Sea which is considered as one of the most ecologically important wetland systems in Greece Based on previous studies in this area there is extensive knowledge on the sediment s macrofaunal diversity Also the genetic diversity of specific species has been estimated using molecular markers such as the cytochrome oxidase subunit I COI gene This previous knowledge will be the basis of this metabarcoding project which will employ next generation sequencing for the assessment of the lagoonal biodiversity patterns Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding sparking conversations between the attendees The end of the first day saw flash talks from

    Original URL path: http://www.metabarcoding.org/spip.php?article55 (2016-04-26)
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  • Attempting to characterize marine meiofauna using metabarcoding: what we got and what we wish - metabarcoding.org
    sediment samples from two Mediterranean canyons have been processed for the DNA extraction and the high throughput sequencing of two fragments from the barcodes 18S and COI More than 2000 different sequences were obtained for the 18S marker after cleaning and filtering the sequences 3 000 000 obtained using an Illumina MiSeq platform These sequences belonged to 19 different Phyla of Metazoa with polychaetes and crustaceans being the most abundant in terms of total number of reads Analysis on the abundance and distribution of the different marine benthic groups are currently being carried out We were not successful for the amplification of the COI fragment marker so we are searching for an appropriate COI barcode Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding sparking conversations between the attendees The end of the first day saw flash talks from

    Original URL path: http://www.metabarcoding.org/spip.php?article54 (2016-04-26)
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