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  • metabarcoding.org
    bg br brezhoneg bs bosanski ca català co corsu cpf Kréol réyoné cpf hat Kréyòl Péyi Dayiti cs čeština da dansk de Deutsch en English eo Esperanto es Español eu euskara fa fon fongbè fr français fr fem français féminin gl galego he hr hrvatski hu magyar id Indonesia it italiano it fem italiana ja km lb Lëtzebuergesch my Burmese nl Nederlands oc auv òc auvernhat oc gsc òc gascon oc lms òc lemosin oc lnc òc lengadocian oc ni òc niçard oc ni la òc niçard larg oc ni mis òc nissart mistralenc oc prv òc provençau oc va òc vivaroaupenc pl polski pt Português pt br Português do Brasil ro român ru sk slovenčina sv svenska tr Türkçe vi Ti ng Vi t zh Personal identifiers Login or e mail address Password password forgotten Remember my ID for a few days Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced

    Original URL path: http://www.metabarcoding.org/spip.php?page=login&url=spip.php%3Fauteur104 (2016-04-26)
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  • metabarcoding.org
    password In order to change your password we have to check your identity first Please enter the e mail address associated with this account Your email address Please leave this field empty Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations in the field

    Original URL path: http://www.metabarcoding.org/spip.php?page=spip_pass&lang=en (2016-04-26)
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  • metabarcoding.org
    bg br brezhoneg bs bosanski ca català co corsu cpf Kréol réyoné cpf hat Kréyòl Péyi Dayiti cs čeština da dansk de Deutsch en English eo Esperanto es Español eu euskara fa fon fongbè fr français fr fem français féminin gl galego he hr hrvatski hu magyar id Indonesia it italiano it fem italiana ja km lb Lëtzebuergesch my Burmese nl Nederlands oc auv òc auvernhat oc gsc òc gascon oc lms òc lemosin oc lnc òc lengadocian oc ni òc niçard oc ni la òc niçard larg oc ni mis òc nissart mistralenc oc prv òc provençau oc va òc vivaroaupenc pl polski pt Português pt br Português do Brasil ro român ru sk slovenčina sv svenska tr Türkçe vi Ti ng Vi t zh Personal identifiers Login or e mail address Password password forgotten Remember my ID for a few days Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced

    Original URL path: http://www.metabarcoding.org/spip.php?page=login&url=spip.php%3Fpage%3Dlogin%26amp%3Burl%3D.%252F (2016-04-26)
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  • Assigning and interpreting on aDNA data from a past Greenland ecosystem - metabarcoding.org
    compensate for possible amplification sequencing and damage errors that could inflate the biodiversity present in samples used for meta barcoding studies By denoising high throughput sequence data we removed a large part of the sequences that were produced by error as well as damage Three different denoising approaches were applied and they differ in how they remove sequences Each of them was combined with one of three assignment methods that consisted of two phylogeny based ones SAP and one in prep method OGA and one similarity based EcoTag Results were only accepted if confirmed by two of three pipelines The data that was approved according to these criteria was used for down stream interpretations in an ecological context The results from the assignment studies were used to deduce ancient biodiversity from the Greenland site that is today covered by a thick ice sheet in northwestern Greenland Ancient DNA from the Camp Century ice core provided results of flora and fauna from a once diverse boreal ecosystem Camp Century was drilled on the margin of the supposedly reduced Greenland Ice Sheet during the last interglacial period and the results show that the area remained covered by ice during the previous interglacial the Eemian 130 116KY Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School

    Original URL path: http://www.metabarcoding.org/spip.php?article17 (2016-04-26)
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  • Benthic vs pelagic contribution in the diet of marine benthic primary consumers: a multi tool approach - metabarcoding.org
    carbon and nitrogen fluxes in aquatic ecosystems undergoing multiple anthropogenic influences anthropic river oceanic To try answer to these questions I intend to develop and use several indicator tools such as stable isotopes pigments transfers as well as bar coding The latter one is yet seldom used in marine organism diet determination especially in mollusks In order to determine a working protocol I am currently making test analysis on 5 oysters This involves extracting DNA of their digestive glands as well as within pelagic and benthic seawaters samples their food sources amplify DNA fragments with universal primers at the moment I have selected the Cox1 and Cox3 genes separate DNA fragments contained in oyster digestive glands and seawaters by denaturing gradient gel DGGE This gel will allow us to compare DNA profiles between seawater samples and of oyster digestive gland samples finally select and sequence matching DNA fragments between food sources and the digestive gland in order to identify the algal species really consumed by oysters Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park

    Original URL path: http://www.metabarcoding.org/spip.php?article23 (2016-04-26)
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  • Cat hair: silent witness of crime scene - metabarcoding.org
    from domestic animals Cat hair which is highly electrostatic can give important information on the presence of humans on crime scenes Such samples generally contain highly fragmented DNA in small quantity requiring the development of specific STR markers The aim of this study is to develop new highly specific primers for these microsatellites and new multiplex PCR protocols allowing the amplification of short DNA sequences Genetic identification of the housecat Felis silvestris catus is based on twelve markers Our approach essentially consists in optimising the primers of these existing markers and in elaborating species specific genetic profile which can be used by forensic scientists Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go off to them for the organizational skills warmth and hospitality During the week experienced metabarcoders gave lectures on their trials and tribulations in the field of metabarcoding sparking conversations between the attendees The end of the first day saw flash talks from all participants of the event creating an icebreaker and showing the

    Original URL path: http://www.metabarcoding.org/spip.php?article22 (2016-04-26)
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  • Cryptic lineages or cryptic species in european barcoded bats (Chiroptera)? - metabarcoding.org
    to occur in Europe multiple major cryptic lineages were discovered in several species Some of these lineages diverged by over 10 K2P genetic distance within what is currently treated as a single species However as mitochondrial lineages in general are transmitted clonally by females only and are prone to horizontal transfer in hybridizing taxa it is unclear whether these cryptic lineages represent genetic variants in an otherwise panmictic population or whether they flag the existence of cryptic species within the European bat fauna To test this important issue for biodiversity conservation we used 17 loci of biparentally inherited nuclear markers to estimate the amount of potential gene flow between individuals bearing different barcodes in a European species of Myotis We genotyped 344 individuals from 21 populations sampled across Western Europe and inferred the amount of genetic admixture with Bayesian assignment of individual nuclear genotypes Results suggest that most groups of individuals identified by different barcodes even when found in sympatry do segregate in distinct nuclear gene pools supporting the existence of several cryptic biologically isolated species within the European bat fauna Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique

    Original URL path: http://www.metabarcoding.org/spip.php?article20 (2016-04-26)
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  • Diet analysis of the Mediterranean shag (Phalacrocorax aristotelis desmarestii) using conventional techniques and next generation sequencing - metabarcoding.org
    is carried out using both conventional prey identification methods and next generation sequencing Regurgitates undigested prey remains and faeces have been collected from a large colony of the species in the N Aegean Sea A conventional prey analysis has been carried out by identifying indigestible part remains mainly fish otoliths from regurgitates The latter as well as faecal samples will be subjected to amplicon sequencing using the 454 GS FLX platform following the protocol of Deagle et al 2009 By analyzing the same samples i e regurgitates both by conventional and molecular techniques we will be able to check whether molecular approaches perform better for prey identification at the species level and if they can give reliable quantitative estimations Moreover the analysis of faecal samples will show whether there are prey items that are not detected in the regurgitates Finally we will estimate which approach is more cost effective for determining the diet of the Mediterranean shag Twitter Metabarcoding Editorial board BRANDNER Melissa University of Nordland Bodø Norway BROCHMANN Christian National Centre for Biosystematics Oslo Norway CHARITON Anthony CSIRO Land and Water Lucas Heights NA Australia DEAGLE Bruce University of Victoria Victoria Canada Eric Coissac LECA Grenoble France KASAPIDIS Panagiotis Hellenic Center for Marine Research Irakleion Crete Greece PAWLOWSKI Jan Université de Genève Genève 4 Switzerland TABERLET Pierre LECA Grenoble France WILLERSLEV Eske Centre for GeoGenetics Copenhagen Denmark Editorial Vodka Bison and Metabarcoding 31 July 2015 by BRANDNER Melissa The start of last month saw the occurrence of the Fifth Metabarcoding Spring School held in Białowieża National Park Poland A variety of scientists attended from all over the globe to learn share and inspire with unique stories of metabarcoding The scientists at the Mammal Research Institute PAS in Białowieża National Park hosted this year s workshop And our hats go

    Original URL path: http://www.metabarcoding.org/spip.php?article18 (2016-04-26)
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