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  • Gram staining technique
    Responsibilities Center of Excellence in Nanotechnology Gram staining technique Friday 12 November 2010 02 59 Mayuree Jaisai Description Use Gram staining is an empirical method of differentiating bacterial species into two large groups Gram positive and Gram negative based on the chemical and physical properties of their cell walls The Gram stain is almost always the first step in the identification of a bacterial organism While Gram staining is a valuable diagnostic tool in both clinical and research settings not all bacteria can be definitively classified by this technique thus forming Gram variable and Gram indeterminate groups as well Safety Before start the experiments always spray 70 alcohol on the table and inside laminar hood While performing the experiments always wear lab safety glasses to prevent chemical irritants from entering the eyes and facemask for protect snuff spores in your body Use lab gown to avoid damage of your clothes Wear lab shoes or slippers to prevent any cut or burns that may occur while handling chemicals and lab equipments Use protective gloves while handling high temperature equipments such as oven hot plate etc Gloves must be removed or changed before touching any other laboratory surfaces or equipment and washing

    Original URL path: http://nanoprof.org/index.php?option=com_content&view=article&id=230:gram-staining-technique&catid=75:protocol-front-page&Itemid=72 (2016-02-13)
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  • Media preparation
    the entry on each strain when first subculturing to ensure optimal conditions for recovery Unless otherwise specified the media are sterilized by autoclaving at 121 C for 15 minutes at 15 lbs pressure Safety Before start the experiments always spray 70 alcohol on the table and inside laminar hood While performing the experiments always wear lab safety glasses to prevent chemical irritants from entering the eyes and facemask for protect

    Original URL path: http://nanoprof.org/index.php?option=com_content&view=article&id=229:media-preparation&catid=75:protocol-front-page&Itemid=72 (2016-02-13)
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  • Fabrication of Anodic Aluminum Oxide (AAO) Membrane
    5 cm width 5 cm length and 2 mm thickness are smoothen by mechanical polishing and electropolishing followed by annealing in order to increase the grain size of aluminum Changing in electrolyte concentration applied voltage and temperature results in variations in the pore regularity pore size and inter pore distance as well as pore density Safety While performing the experiments always wear lab safety glasses to prevent chemical irritants from entering the eyes Use lab gown to avoid damage of your clothes Wear lab shoes or slippers to prevent any cut or burns that may occur while handling chemicals and lab equipments Use protective gloves while handling high temperature equipments such as oven hot plate etc Gloves must be removed or changed before touching any other laboratory surfaces or equipment Take utmost care while handling various chemicals Short safety information for the chemicals used during the experiments is given Perchloric acid is a very strong oxidizer and it can promote combustion Contact with combustible or flammable materials can cause fire or explosion It can react violently or explosively with many organic and inorganic chemicals Vapors or mists are severely irritating to the respiratory tract It is corrosive to the eyes and skin and may cause blindness severe burns and permanent scarring Ethanol is flammable liquid and vapor It may cause central nervous system depression It causes severe eye and respiratory tract irritation and causes moderate skin irritation This substance has caused adverse reproductive and fetal effects in humans Oxalic acid is corrosive and causes severe irritation This chemical can burn to the skin eyes and respiratory tract It is harmful if inhaled or absorbed with skin and may cause kidney damage Oxalic acid reacts explosively with strong oxidizing materials and some silver compounds Phosphoric acid is very hazardous in case

    Original URL path: http://nanoprof.org/index.php?option=com_content&view=article&id=205:fabrication-of-anodic-aluminum-oxide-aao-membrane&catid=75:protocol-front-page&Itemid=72 (2016-02-13)
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  • Hydrothermal synthesis of zinc oxide (ZnO) nanorods
    µm and density 3 x 10 8 rods cm 2 grown on a glass substrate of size 9 cm x 2 5 cm following a hydrothermal route The reagents used are 1mM zinc acetate solution 10mM Hexamine solution and 10mM Zn nitrate solution Changing the concentrations of the reagents and other parameters such as temperature pH etc will result in variations in the size density and orientation of the nanorods Safety While performing the experiments always wear lab safety glasses to prevent chemical irritants from entering the eyes Use lab gown to avoid damage of your clothes Wear lab shoes or slippers to prevent any cut or burns that may occur while handling chemicals and lab equipments Use protective gloves while handling high temperature equipments such as oven hot plate etc Gloves must be removed or changed before touching any other laboratory surfaces or equipment Take utmost care while handling various chemicals Short safety information for the chemicals used during the experiments is given Acetone is extremely flammable liquid and can cause acute low or chronic toxicity if ingested and or inhaled Inhalation of high concentrations around 9200 ppm in the air causes irritation of the throat in humans in as little as 5 minutes Isopropanol vapor is highly flammable and it should not be kept near open flame When mixed with oxidizers such as air it can explode Long term application to the skin can cause defatting Methanol is toxic by inhalation ingestion or skin absorption maybe a reproductive hazard Ingestion may be fatal There is a risk of very serious irreversible damage if swallowed Exposure may cause eye kidney heart and liver damage Zinc acetate is relatively safer though its harmful if swallowed or inhaled It can cause irritation to skin eyes and respiratory tract Hexamine is flammable

    Original URL path: http://nanoprof.org/index.php?option=com_content&view=article&id=200:hydrothermal-synthesis-of-zinc-oxide-zno-nanoparticles&catid=75:protocol-front-page&Itemid=72 (2016-02-13)
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  • Synthesis of gold (Au) nanoparticles
    are stable for several months in the colloidal form Au nanoparticles prepared by this method are obtained from reduction of gold chloride salt by citrate ions and these nanoparticles are electro statically stabilized in the colloidal system The reagents used for the synthesis are gold chloride hydrate HAuCl 4 H 2 O trisodium citrate Na 3 C 6 H 5 O 7 2H 2 O and de ionized water DI water as solvent Safety While performing the experiments always wear lab safety glasses to prevent chemical irritants from entering the eyes Use lab gown to avoid damage of your clothes Wear lab shoes or slippers to prevent any cut or burns that may occur while handling chemicals and lab equipments Use protective gloves while handling high temperature equipments such as oven hot plate etc Gloves must be removed or changed before touching any other laboratory surfaces or equipment Take utmost care while handling various chemicals Short safety information for the chemicals used during the experiments is given Gold chloride is harmful if swallowed and causes severe skin burns and eye damage Wear protective gloves protective clothing eye protection face protection while handling this chemical Trisodium citrate is slightly hazardous in

    Original URL path: http://nanoprof.org/index.php?option=com_content&view=article&id=198:synthesis-of-gold-au-nanoparticles&catid=75:protocol-front-page&Itemid=72 (2016-02-13)
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  • Synthesis of zinc oxide (ZnO) nanoparticles
    nanoparticles synthesized by this method is in the range of 5 6 nm and they are stable for about 1 week in the colloidal form The reagents used for the synthesis are zinc acetate Zn CH 3 COO 2 2H 2 O sodium hydroxide NaOH and ethanol C 2 H 5 OH as solvent Safety While performing the experiments always wear lab safety glasses to prevent chemical irritants from entering the eyes Use lab gown to avoid damage of your clothes Wear lab shoes or slippers to prevent any cut or burns that may occur while handling chemicals and lab equipments Use protective gloves while handling high temperature equipments such as oven hot plate etc Gloves must be removed or changed before touching any other laboratory surfaces or equipment Take utmost care while handling various chemicals Short safety information for the chemicals used during the experiments is given Zinc acetate is hazardous in case of eye contact irritant slightly hazardous in case of skin contact irritant of ingestion of inhalation and may be combustible at high temperature Sodium hydroxide is very hazardous in case of skin contact corrosive irritant permeator of eye contact irritant corrosive of ingestion of inhalation Eye

    Original URL path: http://nanoprof.org/index.php?option=com_content&view=article&id=199:synthesis-of-zinc-oxide-zno-nanoparticles&catid=75:protocol-front-page&Itemid=72 (2016-02-13)
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  • Synthesis of silica (SiO2) nanoparticles
    nm Silica or silicon dioxide SiO 2 is an oxide of silicon Si and widely used for glass manufacturing electronic substrates thin film substrates electrical insulators thermal insulators humidity sensors etc In nature it is most commonly found as sand or quartz The reagents used for the synthesis are tetraethyl orthosilicate TEOS SiC 8 H 20 O 4 ammonium hydroxide NH 4 OH 25 and de ionized water DI water and ethanol as solvents Safety While performing the experiments always wear lab safety glasses to prevent chemical irritants from entering the eyes Use lab gown to avoid damage of your clothes Wear lab shoes or slippers to prevent any cut or burns that may occur while handling chemicals and lab equipments Use protective gloves while handling high temperature equipments such as oven hot plate etc Gloves must be removed or changed before touching any other laboratory surfaces or equipment Take utmost care while handling various chemicals Short safety information for the chemicals used during the experiments is given TEOS is a flammable chemical and harmful if inhaled It creates skin and eye irritant and long term exposure may cause kidney or liver damage Ammonium hydroxide is poisonous dangerous and corrosive

    Original URL path: http://nanoprof.org/index.php?option=com_content&view=article&id=197:synthesis-of-gold-nanoparticles&catid=75:protocol-front-page&Itemid=72 (2016-02-13)
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  • Manual Uploading Weekly Report
    3 Open your browser and go to http www nano ait ac th 4 Scroll down and Login Fill in your name and password and click Login 5 Scroll down again and click menu Upload Weekly Report 6 Scroll down Folder to CoE WeeklyReport2011 7 Click CoE WeeklyReport2011 8 Click the folder according work week 9 Click Add Files 10 Pick your file and click Open 11 Click Upload 12

    Original URL path: http://nanoprof.org/index.php?option=com_content&view=article&id=377:manual-uploading-weekly-report&catid=75:protocol-front-page&Itemid=72 (2016-02-13)
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