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  • Pectic polysaccharides are attacked by hydroxyl radicals in ripening fruit: evidence from a fluorescent fingerprinting method
    was produced in all species usually increasing during fruit softening The six true fruits also gave pAMAC UA GalA disaccharides where pAMAC UA is an unspecified uronate labelled at a position other than carbon 1 with yields increasing during softening Among false fruits apple and strawberry gave little pAMAC UA GalA pear produced it transiently Conclusions GalA pAMAC arises from pectic reducing termini formed by any of three proposed chain cleaving agents OH endopolygalacturonase and pectate lyase any of which could cause its ripening related increase In contrast pAMAC UA GalA conjugates are diagnostic of mid chain oxidation of pectins by OH The evidence shows that OH radicals do indeed attack fruit cell wall polysaccharides non enzymically during softening in vivo This applies much more prominently to drupes and berries true fruits than to false fruits swollen receptacles OH radical attack on polysaccharides is thus predominantly a feature of ovary wall tissue Key words Fruit ripening cell wall pectic polysaccharides hydroxyl radicals non enzymic scission fluorescent labelling fingerprint compounds The Author 2016 Published by Oxford University Press on behalf of the Annals of Botany Company All rights reserved For Permissions please email journals permissions oup com This Article Ann Bot 2016 doi 10 1093 aob mcv192 First published online February 9 2016 Abstract Free Free Figures Free Full Text HTML Full Text PDF Supplementary Data Classifications Article Services Article metrics Alert me when cited Alert me if corrected Alert me if commented Find similar articles Similar articles in PubMed Add to my archive Download citation Request Permissions Responses Submit a response No responses published Citing Articles Load citing article information Citing articles via CrossRef Citing articles via Scopus Google Scholar Articles by Airianah O B Articles by Fry S C PubMed PubMed citation Articles by Airianah O B Articles by Vreeburg R A Articles by Fry S C Agricola Articles by Airianah O B Articles by Fry S C Related Content Load related web page information Share Email this article CiteULike Delicious Facebook Google Mendeley Twitter What s this Search this journal Advanced Current Issue February 2016 117 2 Alert me to new issues The Journal About this journal Annals of Botany Collections AoB article attracts media coverage We are mobile find out more Journals Career Network Published on behalf of The Annals of Botany Company Impact factor 3 654 5 Yr impact factor 4 338 Eigenfactor 0 02603 Rank 10 200 SCImago Score 1 461 Rank 124 1873 Chief Editor Professor J S Pat Heslop Harrison View full editorial board International Review Board For Authors Submitting a manuscript online Self archiving policy Instructions for authors Low Rate Open Access Fees Open access options for authors visit Oxford Open Visit HighWire Press 3hWaciBYRk30rSOQ7UOpP6viAxsZnEle true Looking for your next opportunity Looking for jobs Alerting Services Email table of contents Email Advance Access CiteTrack XML RSS feed Rights Permissions This journal is a member of the Committee on Publication Ethics COPE Corporate Services Advertising sales Reprints Supplements Widget Get a Widget Most

    Original URL path: https://aob.oxfordjournals.org/content/early/2016/02/09/aob.mcv192.abstract (2016-02-18)
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    Original URL path: https://aob.oxfordjournals.org/content/early/2016/02/09/aob.mcv192.full (2016-02-18)
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    uses OpenAthens you can log in using your OpenAthens username and password Contact your library for more details List of OpenAthens registered sites including contact details Login via Your Institution Login via your institution You may be able to gain access using your login credentials for your institution Contact your library if you do not have a username and password Register or Subscribe Subscribe to the Journal Subscribe to the print and or online journal Register Register online for access to selected content and to use Pay per View Registration is free This Article Ann Bot 2016 doi 10 1093 aob mcv192 First published online February 9 2016 Show PDF in full window Abstract Free Free Figures Free Full Text HTML Full Text PDF Supplementary Data Classifications Article Services Article metrics Alert me when cited Alert me if corrected Alert me if commented Find similar articles Similar articles in PubMed Add to my archive Download citation Request Permissions Responses Submit a response No responses published Citing Articles Load citing article information Citing articles via CrossRef Citing articles via Scopus Google Scholar Articles by Airianah O B Articles by Fry S C PubMed PubMed citation Articles by Airianah O B Articles by Vreeburg R A Articles by Fry S C Agricola Articles by Airianah O B Articles by Fry S C Related Content Load related web page information Share Email this article Search this journal Advanced Current Issue February 2016 117 2 Alert me to new issues The Journal About this journal Annals of Botany Collections AoB article attracts media coverage We are mobile find out more Journals Career Network Published on behalf of The Annals of Botany Company Impact factor 3 654 5 Yr impact factor 4 338 Eigenfactor 0 02603 Rank 10 200 SCImago Score 1 461 Rank 124

    Original URL path: https://aob.oxfordjournals.org/content/early/2016/02/09/aob.mcv192.full.pdf+html (2016-02-18)
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  • Pectic polysaccharides are attacked by hydroxyl radicals in ripening fruit: evidence from a fluorescent fingerprinting method
    each fruit sample shows up as a dark spot under UV anode cathode blank loading View larger version In this window In a new window Download as PowerPoint Slide F ig 4 HPLC of total Driselase digests of pAMAC labelled cell walls from three fruit species AIRs from pear mango and banana fruit stages 3 1 and 1 respectively were treated with AMAC acetone Driselase Supelco C 18 and NaOH all as in Fig 3 Total fluorescent products which will include conjugates of both neutral and acidic carbohydrates were analysed by HPLC A before and B after addition of a marker mixture containing acidic sugar pAMAC conjugates Fluorescence detection was with excitation at 442 nm and emission at 520 nm MM marker mixture containing authentic acidic sugar pAMAC conjugates Green arrows authentic sugar pAMACs including those added as a spike blue arrows unidentified peaks from fruit cell wall digests thick purple arrows with asterisk putative GalA pAMAC from fruit cell wall digests View larger version In this window In a new window Download as PowerPoint Slide F ig 5 HPLC of the acidic monomer 1A F spots from Driselase digested pAMAC labelled cell walls of seven fruit species Each 1A F spot pooled for all three stages of development for each fruit de lactonized shown in Fig 3 was eluted from the electrophoretogram and analysed by HPLC MM marker mixture containing authentic acidic sugar pAMAC conjugates Arrows putative GalA pAMAC and its lactone which partially re formed during elution from the electrophoretogram from fruit cell wall digests Dashed lines compounds Y and Z discussed in the text View larger version In this window In a new window Download as PowerPoint Slide F ig 6 HPLC of the acidic dimer 2A F spots from Driselase digested pAMAC labelled cell walls of five fruit species 2A F spots were eluted from a paper electrophoretogram similar to that shown in Fig 3 but derived from non de lactonized samples all three ripening stages combined and analysed by HPLC MM marker mixture containing authentic acidic sugar pAMAC conjugates Arrows the proposed fingerprints for OH attack pAMAC UA GalA and its lactone rapidly re formed during elution from the electrophoretogram from fruit cell wall digests Dashed lines predicted position of authentic GalA 2 pAMAC and GalA 2 lactone pAMAC deduced from the marker run The samples in the upper and lower graphs were run on different days accounting for the slight discrepancy in marker retention times Strawberry and apple were not included because they did not show any appreciable 2A F spot in Fig 3 View larger version In this window In a new window Download as PowerPoint Slide F ig 7 HPLC of the acidic unknown X F spots from Driselase digested pAMAC labelled cell walls of banana and Arbutus The X F spot similar to that shown in Fig 3 but from a non de lactonized sample for stage 1 banana and Arbutus was eluted from an electrophoretogram and analysed by HPLC MM

    Original URL path: https://aob.oxfordjournals.org/content/early/2016/02/09/aob.mcv192.figures-only (2016-02-18)
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  • Does light influence the relationship between a native stem hemiparasite and a native or introduced host?
    level of parasite autotrophy on growth of hosts Despite the lower levels of photosynthesis in the parasite the relative impact of infection on host biomass was the same in both LL and HL In fact biomass of L myrsinoides was unaffected by infection in either HL or LL while biomass of U europaeus was negatively affected by infection in both treatments This suggests that although photosynthesis of the parasite was lower in LL there was no additional impact on host biomass in LL In addition light did not affect the amount of parasite biomass supported per unit host biomass in either host although this parameter was slightly lower in LL than HL for U europaeus P 0 073 We also found no significant enhancement of host photosynthesis in response to infection in either host regardless of light treatment Conclusions Despite lower photosynthetic rates in LL C pubescens did not increase its dependency on host C to the point where it affected host growth or photosynthesis The impact of C pubescens on host growth would be similar in areas of high and low light availability in the field but the introduced host is more negatively affected by infection Key words Biomass Cassytha pubescens gas exchange hemiparasite host association Leptospermum myrsinoides light nitrogen photosynthesis Ulex europaeus water potential The Author 2016 Published by Oxford University Press on behalf of the Annals of Botany Company All rights reserved For Permissions please email journals permissions oup com This Article Ann Bot 2016 doi 10 1093 aob mcv193 First published online February 1 2016 Abstract Free Free Figures Free Full Text HTML Full Text PDF Supplementary Data Classifications Article Services Article metrics Alert me when cited Alert me if corrected Alert me if commented Find similar articles Similar articles in PubMed Add to my archive Download citation Request Permissions Responses Submit a response No responses published Citing Articles Load citing article information Citing articles via CrossRef Citing articles via Scopus Google Scholar Articles by Cirocco R M Articles by Watling J R Search for related content PubMed PubMed citation Articles by Cirocco R M Articles by Facelli J M Articles by Watling J R Agricola Articles by Cirocco R M Articles by Watling J R Related Content Load related web page information Share Email this article CiteULike Delicious Facebook Google Mendeley Twitter What s this Search this journal Advanced Current Issue February 2016 117 2 Alert me to new issues The Journal About this journal Annals of Botany Collections AoB article attracts media coverage We are mobile find out more Journals Career Network Published on behalf of The Annals of Botany Company Impact factor 3 654 5 Yr impact factor 4 338 Eigenfactor 0 02603 Rank 10 200 SCImago Score 1 461 Rank 124 1873 Chief Editor Professor J S Pat Heslop Harrison View full editorial board International Review Board For Authors Submitting a manuscript online Self archiving policy Instructions for authors Low Rate Open Access Fees Open access options for authors visit Oxford Open

    Original URL path: https://aob.oxfordjournals.org/content/early/2016/01/31/aob.mcv193.abstract (2016-02-18)
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    uses OpenAthens you can log in using your OpenAthens username and password Contact your library for more details List of OpenAthens registered sites including contact details Login via Your Institution Login via your institution You may be able to gain access using your login credentials for your institution Contact your library if you do not have a username and password Register or Subscribe Subscribe to the Journal Subscribe to the print and or online journal Register Register online for access to selected content and to use Pay per View Registration is free This Article Ann Bot 2016 doi 10 1093 aob mcv193 First published online February 1 2016 Abstract Free Free Figures Free Full Text HTML Full Text PDF Supplementary Data Classifications Article Services Article metrics Alert me when cited Alert me if corrected Alert me if commented Find similar articles Similar articles in PubMed Add to my archive Download citation Request Permissions Responses Submit a response No responses published Citing Articles Load citing article information Citing articles via CrossRef Citing articles via Scopus Google Scholar Articles by Cirocco R M Articles by Watling J R Search for related content PubMed PubMed citation Articles by Cirocco R M Articles by Facelli J M Articles by Watling J R Agricola Articles by Cirocco R M Articles by Watling J R Related Content Load related web page information Share Email this article Search this journal Advanced Current Issue February 2016 117 2 Alert me to new issues The Journal About this journal Annals of Botany Collections AoB article attracts media coverage We are mobile find out more Journals Career Network Published on behalf of The Annals of Botany Company Impact factor 3 654 5 Yr impact factor 4 338 Eigenfactor 0 02603 Rank 10 200 SCImago Score 1 461 Rank 124 1873

    Original URL path: https://aob.oxfordjournals.org/content/early/2016/01/31/aob.mcv193.full (2016-02-18)
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    can log in using your OpenAthens username and password Contact your library for more details List of OpenAthens registered sites including contact details Login via Your Institution Login via your institution You may be able to gain access using your login credentials for your institution Contact your library if you do not have a username and password Register or Subscribe Subscribe to the Journal Subscribe to the print and or online journal Register Register online for access to selected content and to use Pay per View Registration is free This Article Ann Bot 2016 doi 10 1093 aob mcv193 First published online February 1 2016 Show PDF in full window Abstract Free Free Figures Free Full Text HTML Full Text PDF Supplementary Data Classifications Article Services Article metrics Alert me when cited Alert me if corrected Alert me if commented Find similar articles Similar articles in PubMed Add to my archive Download citation Request Permissions Responses Submit a response No responses published Citing Articles Load citing article information Citing articles via CrossRef Citing articles via Scopus Google Scholar Articles by Cirocco R M Articles by Watling J R Search for related content PubMed PubMed citation Articles by Cirocco R M Articles by Facelli J M Articles by Watling J R Agricola Articles by Cirocco R M Articles by Watling J R Related Content Load related web page information Share Email this article Search this journal Advanced Current Issue February 2016 117 2 Alert me to new issues The Journal About this journal Annals of Botany Collections AoB article attracts media coverage We are mobile find out more Journals Career Network Published on behalf of The Annals of Botany Company Impact factor 3 654 5 Yr impact factor 4 338 Eigenfactor 0 02603 Rank 10 200 SCImago Score 1 461 Rank

    Original URL path: https://aob.oxfordjournals.org/content/early/2016/01/31/aob.mcv193.full.pdf+html (2016-02-18)
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  • Does light influence the relationship between a native stem hemiparasite and a native or introduced host?
    on in situ A B and g s D of L myrsinoides open bars average of HL and LL uninfected plants pooled grey bars average of HL and LL infected plants pooled F Independent effect of light on in situ A of U europaeus in HL dark grey bars average of uninfected and infected HL plants pooled versus LL black bars average of uninfected and infected LL plants pooled Letters indicate significant differences bars are means 1 s e and n 5 A C 10 B D 6 E G except uninfected HL plants n 5 and 11 12 F View larger version In this window In a new window Download as PowerPoint Slide F ig 4 Total shoot open bars and root grey bars biomass of L myrsinoides A and U europaeus B grown in high HL or low light LL and uninfected minus or infected plus with C pubescens C Independent effect of infection on total shoot open dotted bar and root biomass dotted grey bars of U europaeus left bar average of uninfected HL and LL plants pooled right bar average of infected HL and LL plants pooled D Independent effect of light on total shoot open dotted bar and root biomass black bars of U europaeus left bar average of uninfected and infected HL plants pooled right bar average of uninfected and infected LL plants pooled Letters indicate significant differences for total a c shoot l n and root x z biomass bars are means 1 s e and n 5 A 6 B and 12 C D View larger version In this window In a new window Download as PowerPoint Slide F ig 5 Total biomass and grams of parasite dry weight per gram of host dry weight respectively of C pubescens growing on L myrsinoides A B or U europaeus C D in high HL dark grey bars or low light LL black bars Letters indicate significant differences bars are means 1 s e and n 5 A B and 6 C D The Author 2016 Published by Oxford University Press on behalf of the Annals of Botany Company All rights reserved For Permissions please email journals permissions oup com This Article Ann Bot 2016 doi 10 1093 aob mcv193 First published online February 1 2016 Abstract Free Free Figures Free Full Text HTML Full Text PDF Supplementary Data Classifications Article Services Article metrics Alert me when cited Alert me if corrected Alert me if commented Find similar articles Similar articles in PubMed Add to my archive Download citation Request Permissions Responses Submit a response No responses published Citing Articles Load citing article information Citing articles via CrossRef Citing articles via Scopus Google Scholar Articles by Cirocco R M Articles by Watling J R Search for related content PubMed PubMed citation Articles by Cirocco R M Articles by Facelli J M Articles by Watling J R Agricola Articles by Cirocco R M Articles by Watling J R Related Content Load related web page information

    Original URL path: https://aob.oxfordjournals.org/content/early/2016/01/31/aob.mcv193.figures-only (2016-02-18)
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